Fisheries Science

, Volume 72, Issue 3, pp 485–493 | Cite as

Control of dissolved oxygen levels of water in net pens for fish farming by a microscopic bubble generating system

  • Sarawut Srithongouthai
  • Akira Endo
  • Akihiro Inoue
  • Kyoko Kinoshita
  • Miho Yoshioka
  • Ayako Sato
  • Takaaki Iwasaki
  • Ichiro Teshiba
  • Hisatsune Nashiki
  • Daigo Hama
  • Hiroaki TsutsumiEmail author


A microscopic bubble generating system (MBGS) has been developed to control dissolved oxygen (DO) levels suitable for fish farming. The MBGS has been tested to confirm its capability in net pens. Water conditions in a fish farm were monitored every two hours from June to October 2004 by setting an online vertical profiling system (OVPS) close to the net pen. DO in the net pen water decreased to physiologically stressful levels for the fish during the night (4.84–5.51 mg/L), while the DO was kept in saturated conditions during the day, due to oxygen supply from phytoplankton. The MBGS was operated from the evening to the morning of the next day for 16 h, to successfully create DO-saturated conditions in the net pen water at night. By using microscopic bubbles during the warm seasons, DO levels in the net pen water could be improved to a level suitable for fish farming. However, the low DO levels (<5.0 mg/L) of the bottom water occasionally extended to the net pen layers, despite the supply of microscopic bubbles to the water. To maintain the DO of the net pen water at levels suitable for fish farming, DO supply to the net pen water and the bottom water needs to be increased, and the organically enriched sediment just below the net pens needs to be treated.

Key Words

dissolved oxygen fish farming microscopic bubble online vertical profiling system red sea bream 


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  1. 1.
    Wu RSS. The environmental impact marine fish culture: towards a sustainable future. Mar. Poll. Bull. 1995; 31: 159–166.CrossRefGoogle Scholar
  2. 2.
    Ministry of Agriculture Forestry and Fisheries. Statistics of agriculture, forestry and fisheries. 2004; [cited 5 December 2004.] Available from URL: sokuhou/data/gyogyou-yousyoku2003/gyogyou-yousyoku2003.html.Google Scholar
  3. 3.
    Brown JR, Gowen RJ, McLusky DS. The effect of salmon farming on the benthos of a Scottish sea loch. J. Exp. Mar. Biol. Ecol. 1987; 109: 39–51.CrossRefGoogle Scholar
  4. 4.
    Weston DP. Quantitative examination of macrobenthic community changes along an organic enrichment gradient. Mar. Ecol. Prog. Ser. 1990; 61: 233–244.CrossRefGoogle Scholar
  5. 5.
    Ye LX, Ritz DA, Fenton GE, Lewis ME. Tracing the influence on sediments of organic waste from a salmonid farm using stable isotope analysis. J. Exp. Mar. Biol. Ecol. 1991; 145: 161–174.CrossRefGoogle Scholar
  6. 6.
    Cornel GE, Whoriskey FG. The effects of rainbow trout (Oncorhynchus mykiss) cage culture on the water quality, zooplankton, benthos and sediments of Lac du Passage, Quebec. Aquaculture 1993; 109: 101–117.CrossRefGoogle Scholar
  7. 7.
    Hargrave BT, Duplisea DE, Pfeiffer E, Wildish DJ. Seasonal changes in benthic fluxes of dissolved oxygen and ammonium associated with marine cultured Atlantic salmon. Mar. Ecol. Prog. Ser. 1993; 96: 249–257.CrossRefGoogle Scholar
  8. 8.
    Hirata H, Kadowaki S, Ishida S. Evaluation of water quality by observation of dissolved oxygen content in mariculture farms. Bull. Natl. Res. Inst. Aquacult. 1994; (Suppl. 1): 61–65.Google Scholar
  9. 9.
    Findlay RH, Watling L. Environmental impact of salmon net-pen culture on marine benthic communities in marine; A case study. Estuaries 1995; 18: 145–179.CrossRefGoogle Scholar
  10. 10.
    Tsutsumi H, Kikuchi T. Benthic ecology of a small cove with seasonal oxygen depletion caused by organic pollution. Publ. Amakusa Mar. Biol. Lab. 1983; 7: 17–40.Google Scholar
  11. 11.
    Tsutsumi H, Kikichi T, Tanaka M, Hgashi T, Imasaka K, Miyazaki M. Benthic faunal succession in a cove organically polluted by fish farming. Mar. Poll. Bull. 1991; 23: 233–238.CrossRefGoogle Scholar
  12. 12.
    Diaz RJ, Rosenberg R. Marine benthic hypoxia: a review of its ecological effects and the behavioral responses of benthic macrofauna. Oceanogr. Mar. Biol. 1985; 33: 245–303. (An annual review).Google Scholar
  13. 13.
    Murata O. The present conditions of mariculture, red sea bream. In: Kumai O (ed.). Saishin Kaisangyo no Yoshoku (The Latest Marine Fish Culture). Bunshodo, Tokyo. 2000; 89–108 (in Japanese).Google Scholar
  14. 14.
    Kubota T. Fish net pen culture. In: the Japanese Society of Scientific Fisheries (ed.). Senkai Yoshoku to Jikaosen (Fish Culture in Coastal Areas and Self-Pollution). Kouseisha-Kouseikaku, Tokyo, 1977; 9–18 (in Japanese).Google Scholar
  15. 15.
    Kawai A, Kitada H, Maeda H. Restoration techniques of a small area in polluted fish farms. In: Watanabe T (ed.). Coastal Fish Farms and the Environment. Kouseisha-Kouseikaku. Tokyo. 1990; 110–121 (in Japanese).Google Scholar
  16. 16.
    Kimura H. Restoration techniques of polluted fish farms. In: Watanabe T (ed.). Coastal Fish Farms and the Environment. Kouseisha-Kouseikaku. Tokyo. 1990; 99–109 (in Japanese).Google Scholar
  17. 17.
    Ruangdej U, Fukami K. Stimulation of photosynthesis and consequent oxygen production in anoxic bottom water by supply of low-intensity light through an optical fiber. Fish. Sci. 2004; 70: 421–429.CrossRefGoogle Scholar
  18. 18.
    Onari H. Fisheries experiment of cultivated shells using micro-bubble techniques. J. Heat Transfer Soc. Jpn. 2001; 40: 2–7.Google Scholar
  19. 19.
    Takahira H, Okura E, Nagata T. Influence of gas diffusion on the stability and merger of microbubbles. Fifth Int. Symp. on Cavitation (CAV2003), Osaka. 2003; November 1–4: 1–7.Google Scholar
  20. 20.
    Sadatomi M, Kawahara A, Kano K, Ohtomo A. Performance of a new micro-bubble generator with a spherical body in a flowing water tube. Exp. Therm. Fluid Sci. 2005; 29: 615–623.CrossRefGoogle Scholar
  21. 21.
    Harada T. Yellowtail and amberjack. In: Kawamoto N (ed.). Details of Fish Culture. Kouseisha-Kouseikaku. Tokyo, 1978; 463–503.Google Scholar
  22. 22.
    Hirata H, Kadowaki S. DO management in coastal fish farms. In: Watanabe T (ed.). Coastal Fish Farms and the Environment. Kouseisha-Kouseikaku, Tokyo. 1990; 28–38 (in Japanese).Google Scholar
  23. 23.
    Gillibrand PA, Turrell WR, Moore DC, Adams RD. Bottom water stagnation and oxygen depletion in a Scottish Sea Loch. Estuar. Coast. Shelf Sci. 1996; 43: 217–235.CrossRefGoogle Scholar
  24. 24.
    La Rosa T, Mirto S, Favaloro E, Savona B, Sara G, Danovaro R, Mazzol A. Impact on the water column biogeochemistry of a Mediterranean mussel and fish farm. Water Res. 2002; 36: 713–721.CrossRefGoogle Scholar
  25. 25.
    Sakami T, Abo K, Takayanagi K, Toda S. Effects of water mass exchange on bacterial communities an aquaculture area during summer. Estuar. Coast Sci. 2003; 56: 111–118.CrossRefGoogle Scholar
  26. 26.
    Yokoyama H. Environmental quality criteria for fish Farms in Japan. Aquaculture 2003; 226: 45–56.CrossRefGoogle Scholar
  27. 27.
    Hall POJ, Anderson LG, Holby O, Kollberg S, Samuelsson M Chemical fluxes and mass balances in a marine fish cage farm. I. Carbon. Mar. Ecol. Prog. Ser. 1990; 61: 61–73.CrossRefGoogle Scholar
  28. 28.
    Tsutsumi H. Impact of fish net pen culture on the benthic environment of a cove in south Japan. Estuaries 1995; 18: 108–115.CrossRefGoogle Scholar
  29. 29.
    Karakassis I, Hatziyanni E, Tsapakis M, Plaiti W. Impact of cage farming of fish on the seabed in three Mediterranean coastal areas. ICES J. Mar. Sci. 2000; 57: 1462–1471.CrossRefGoogle Scholar
  30. 30.
    Holmer M, Marbá N, Terrados J, Duarte CM, Fortes MD. Impact of milkfish (Chanos chanos) aquaculture on carbon and nutrient fluxes in the Bolinao area, Philippines. Mar. Poll. Bull. 2002; 44: 685–696.CrossRefGoogle Scholar
  31. 31.
    Tsutsumi H, Kinoshita K, Srithongouthai S, Sato A, Nagata S, Inoue A, Yoshioka M, Ohwada K, Hama D. Treatment of the organically enriched sediment below the fish farm with the biological activities of artificially mass-cultured colonies of a small deposit feeding polychaete, Capitella sp. I.. Benthos Res. 2005; 60: 25–38.Google Scholar

Copyright information

© The Japanese Society of Fisheries Science 2006

Authors and Affiliations

  • Sarawut Srithongouthai
    • 1
  • Akira Endo
    • 2
  • Akihiro Inoue
    • 3
  • Kyoko Kinoshita
    • 3
  • Miho Yoshioka
    • 3
  • Ayako Sato
    • 3
  • Takaaki Iwasaki
    • 2
  • Ichiro Teshiba
    • 2
  • Hisatsune Nashiki
    • 2
  • Daigo Hama
    • 1
  • Hiroaki Tsutsumi
    • 3
    Email author
  1. 1.Keiten Co., Ltd.Hondo, KumamotoJapan
  2. 2.Tashizen Techno Work Co., Ltd.Ishihara, KumamotoJapan
  3. 3.Faculty of Environmental and Symbiotic SciencesPrefectural University of KumamotoTsukide, KumamotoJapan

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