Human Cell

, Volume 22, Issue 3, pp 55–63

Induced in vitro differentiation of pancreatic-like cells from human amnion-derived fibroblast-like cells

  • Tomoharu Tamagawa
  • Isamu Ishiwata
  • Kahei Sato
  • Yukio Nakamura
Research Article

Abstract

There is growing evidence that the human amnion contains various types of stem cells. As amniotic tissue is readily available, it has the potential to be an important source of material for regenerative medicine. In the present study, we evaluated the potential of human amnion-derived fibroblast-like (HADFIL) cells to differentiate into pancreatic islet cells. Two HADFIL cell populations, derived from two different neonates, were analyzed. The expression of pancreatic cell-specific genes was examined before and after in vitro induction of cellular differentiation. We found that Pdx-1, Isl-1, Pax-4, and Pax-6 showed significantly increased expression following the induction of differentiation. In addition, immunostaining demonstrated that insulin, glucagon, and somatostatin were present in HADFIL cells following the induction of differentiation. These results indicate that HADFIL cell populations have the potential to differentiate into pancreatic islet cells. Although further studies are necessary to determine whether such in vitro-differentiated cells can function in vivo as pancreatic islet cells, these amniotic cell populations might be of value in therapeutic applications that require human pancreatic islet cells.

Key words

amnion diabetes mellitus insulin pancreas regenerative medicine 

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References

  1. 1.
    Shapiro AMJ, Lakey JRT, Ryan EA. Islet transplantation in seven patients with type I diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 2000; 343: 230–8.CrossRefPubMedGoogle Scholar
  2. 2.
    Robertson RP. Islet transplantation as a treatment for diabetes: a work in progress. N Engl J Med 2004; 350: 694–705.CrossRefPubMedGoogle Scholar
  3. 3.
    Tamagawa T, Oi S, Ishiwata I et al. Differentiation of mesenchymal cells derived from human amniotic membranes into hepatocyte-like cells in vitro. Hum Cell 2007; 20: 77–84.CrossRefPubMedGoogle Scholar
  4. 4.
    Sudo K, Kanno M, Miharada K et al. Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations. Stem Cells 2007; 25: 1610–17.CrossRefPubMedGoogle Scholar
  5. 5.
    Tamagawa T, Ishiwata I, Nakamura Y et al. Differentiation of human amniotic membrane cells into osteoblasts in vitro. Hum Cell 2005; 18: 191–5.CrossRefPubMedGoogle Scholar
  6. 6.
    Tamagawa T, Ishiwata I, Saito S. Establishment and characterization of a pluripotent stem cell line derived from human amniotic membranes and initiation of germ layers in vitro. Hum Cell 2004; 17: 125–30.CrossRefPubMedGoogle Scholar
  7. 7.
    Tamagawa T, Ishiwata I, Ishikawa H et al. Induced in vitro differentiation of neural-like cells from human amnion-derived fibroblast-like cells. Hum Cell 2008; 21: 38–45.CrossRefPubMedGoogle Scholar
  8. 8.
    Miki T, Lehmann T, Cai H et al. Stem cell characteristics of amniotic epithelial cells. Stem Cell 2005; 23: 1549–59.CrossRefGoogle Scholar
  9. 9.
    Wei JP, Zhang TS, Kawa S et al. Human amnion-isolated cells normalize blood glucose in streptozotocin-induced diabetic mice. Cell Transplantation 2003; 12: 545–52.PubMedGoogle Scholar
  10. 10.
    Hou Y, Haung Q, Liu T et al. Human amnion epithelial cells can be induced to differentiate into functional insulin-producing cells. Acta Biochim Biophys Sin 2008; 40: 830–9.PubMedGoogle Scholar
  11. 11.
    Chang CM, Kao CL, Chang YL et al. Placenta-derived multipotent stem cells induced to differentiate into insulin-positive cells. Biochem Biophys Res Commun 2007; 357: 414–20.CrossRefPubMedGoogle Scholar
  12. 12.
    Sun B, Roh KH, Lee SR et al. Induction of human umbilical cord blood-derived stem cells with embryonic stem cell phenotypes into insulin producing islet-like structure. Biochem Biophys Res Commun 2007; 354: 919–23.CrossRefPubMedGoogle Scholar
  13. 13.
    Timper K, Seboek D, Eberhardt M et al. Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem Biophys Res Commun 2006; 341: 1135–40.CrossRefPubMedGoogle Scholar
  14. 14.
    Yu S, Li C, Xin-guo H et al. Differentiation of bone marrow-derived mesenchymal stem cells from diabetic patients into insulin-producing cells in vitro. Chin Med J 2007; 120: 771–6.Google Scholar
  15. 15.
    Tang DQ, Cao LZ, Burkhardt BR. In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes 2004; 53: 1721–32.CrossRefPubMedGoogle Scholar
  16. 16.
    Kojima H, Fujimiya M, Matsumura K et al. Extrapancreatic insulin-producing cells in multiple organs in diabetes. Proc Natl Acad Sci USA 2004; 101: 2458–63.CrossRefPubMedGoogle Scholar
  17. 17.
    Micallef SJ, Janes ME, Knezavic K et al. Retionic acid induces pdx-1 positive endoderm in differentiating mouse embryonic stem cells. Diabetes 2005; 54: 301–5.CrossRefPubMedGoogle Scholar
  18. 18.
    Gao R, Ustinov J, Pulkkinen MA et al. Characterization of endocrine progenitor cells and critical factors for their differentiation in human adult pancreatic cell culture. Diabetes 2003; 52: 2007–15.CrossRefPubMedGoogle Scholar
  19. 19.
    Shi Y, Hou L, Tang F et al. Inducing embryonic stem cells to differentiate into pancreatic β cell by a novel three-step approach with active A and all-trans retinoic acid. Stem Cells 2005; 23: 656–62.CrossRefPubMedGoogle Scholar
  20. 20.
    Yang L, Li S, Hatch H et al. In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells. Proc Natl Acad Sci USA 2004; 101: 2458–63.CrossRefGoogle Scholar
  21. 21.
    Ramiya VK, Maraist M, Arfors KE et al. Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cell. Nat Med 2000; 6: 278–82.CrossRefPubMedGoogle Scholar
  22. 22.
    Otonkoski T, Beattie GM, Mally MI et al. Nicotinamide is potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J Clin Invest 1993; 92: 1459–66.CrossRefPubMedGoogle Scholar
  23. 23.
    Bonner-Weir S, Taneja M, Weir GC. In vitro cultivation of human islets from expanded ductal tissue. Proc Natl Acad Sci USA 2000; 97: 7999–8004.CrossRefPubMedGoogle Scholar
  24. 24.
    Cras-Meneur C, Elghazi L, Czernichow P et al. Epidermal growth factor increases undifferentiated pancreatic embryonic cells in vitro: a balance between proliferation and differentiation. Diabetes 2001; 50: 1571–9.CrossRefPubMedGoogle Scholar
  25. 25.
    Zarek KS. Molecular genetics of early liver development. Ann Rev Rhysiol 1996; 58: 151–231.Google Scholar
  26. 26.
    Wu K, Gannon M, Peshavaria M et al. Hepatocyte nuclear factor 3β is involved in pancreatic β cell-specific transcription of the pdx-1 gene. Mol Cell Biol 1997; 17: 6002–13.PubMedGoogle Scholar
  27. 27.
    Jacquemin P, Durviaux SM, Jensen J et al. Transcription factor hepatocyte nuclear factor 6 regulates pancreatic endocrine cell differentiation and controls expression of the proendocrine gene ngn 3. Mol Cell Biol 2000; 20: 4445–54.CrossRefPubMedGoogle Scholar
  28. 28.
    Hunzinger E, Stein M. Nestin-expressing cells in the pancreatic islets of Langerhans. Biochem Biophys Res Commun 2000; 271: 116–19.CrossRefGoogle Scholar
  29. 29.
    Zulewski H, Abraham EJ, Gerlach MJ et al. Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine and hepatic phenotypes. Diabetes 2001; 50: 521–33.CrossRefPubMedGoogle Scholar
  30. 30.
    Lumelsky N, Blondel O, Laeng P et al. Differentiation of embryonic stem cell to insulin-secreting structures similar to pancreatic islets. Science 2001; 292: 1389–94.CrossRefPubMedGoogle Scholar
  31. 31.
    Ahlgren U, Pfaff SL, Jessell TM et al. Independent requirement for ISL1 in formation of pancreatic mesenchyme and islet cells. Nature 1997; 385: 257–60.CrossRefPubMedGoogle Scholar
  32. 32.
    Waeber G, Thompson N, Nicod P et al. Transcriptional activation of the GLUT2 gene by the IPF-1/STF-1/ IDX-1 homeobox factor. Mol Endocrinol 1996; 10: 1327–33.CrossRefPubMedGoogle Scholar
  33. 33.
    Li Y, Zhang R, Qiao H et al. Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells. Cell Physiol 2007; 211: 36–44.CrossRefGoogle Scholar
  34. 34.
    Ahlgren U, Pfaff SL, Jessell TM et al. Independent requirement for ISL1 in formation of pancreatic mesenchyme and islet cells. Nature 1997; 385: 257–60.CrossRefPubMedGoogle Scholar
  35. 35.
    Sosa-Pineda B, Chowdhury K, Torres M et al. The Pax4 gene is essential for differentiation of insulin-producing β cell in the mammalian pancreas. Nature 1997; 387: 399–402.CrossRefGoogle Scholar
  36. 36.
    St-Onge L, Sosa-Pineda B, Chowdhury K et al. Pax6 is required for differentiation of glucagons-producing alpha-cells in mouse pancreas. Nature 1997; 387: 406–9.CrossRefPubMedGoogle Scholar

Copyright information

© Society and Springer Japan 2009

Authors and Affiliations

  • Tomoharu Tamagawa
    • 1
    • 2
    • 3
  • Isamu Ishiwata
    • 1
  • Kahei Sato
    • 2
  • Yukio Nakamura
    • 3
  1. 1.Institute of Cell BiologyIshiwata HospitalIbaraki
  2. 2.Department of Applied Life Science, Graduate School of Bioresource SciencesNihon UniversityKanagawa
  3. 3.Cell Engineering DivisionRIKEN BioResource CenterIbarakiJapan

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