Human Cell

, Volume 19, Issue 1, pp 24–29 | Cite as

Cancer stem cells in human gastrointestinal cancers

  • Naotsugu Haraguchi
  • Hiroshi Inoue
  • Fumiaki Tanaka
  • Koshi Mimori
  • Tohru Utsunomiya
  • Atsushi Sasaki
  • Masaki Mori
Feature: Gene Diagnosis — Treatment of Carcinoma


The concept of cancer stem cell has developed in leukemia. Recently, it has expanded to include solid tumors such as brain or breast tumors. However, the descriptions are not recognized in human gastrointestinal cancers. We used flow cytometry and the DNA-binding dye (Hoechst 33342) to isolate side population (SP) cells from various human gastrointestinal system cancer cell lines. The SP cell fraction is considered to contain abundant stem cells. Fifteen of 16 cancer cell lines from the gastrointestinal system contained 0.3–2.2% SP cells. We studied the characteristics of the SP cells in hepatic or colon cancer cell lines. The results demonstrated that cancers of the gastrointestinal system do contain SP cells that show some characters of so-called stem cells. In this paper, we report our study results with a review of the literature.

Key words

chemoresistance flow cytometry microarray side population stem cell 


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Copyright information

© Society and Springer Japan 2006

Authors and Affiliations

  • Naotsugu Haraguchi
    • 1
  • Hiroshi Inoue
    • 1
  • Fumiaki Tanaka
    • 1
  • Koshi Mimori
    • 1
  • Tohru Utsunomiya
    • 1
  • Atsushi Sasaki
    • 1
  • Masaki Mori
    • 1
  1. 1.Department of Surgery, Medical Institute of BioregulationKyushu UniversityBeppuJapan

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