Antioxidative activities of a mycosporine-like amino acid, porphyra-334
- 158 Downloads
Antioxidative activities of porphyra-334, a mycosporine-like amino acid extracted from laver were evaluated. Oxidation of linoleic acid induced by an alkyl-radical 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) was successfully suppressed by porphyra-334 (0–200 μM). The simultaneous application of 0.02 μM α-tocopherol and 50 μM porphyra-334 effectively suppressed the AAPH induced oxidation level to approximately 40% of a single application of porphyra-334 after 10 min reaction. Porphyra-334 (0–200 μM) efficiently suppressed the lipid peroxidation induced by singlet oxygen although the antioxidative effect observed was relatively moderate at the initial stage of oxidation. These results suggested that porphyra-334 may function as an antioxidant which influences the storage stability of laver.
Key wordsantioxidant lipid oxidation mycosporine-like amino acid porphyra-334 singlet oxygen tocopherol
Unable to display preview. Download preview PDF.
- 7.Tsujino I, Yabe K, Sekikawa I. Isolation and structure of a new amino acid, shinorine, from the red alga Chondrus yendoi Yamada Mikami. Bot. Mar. 1980; 23: 65–68.Google Scholar
- 8.Bandaranayake WM, Bourne DJ, Sim RG. Chemical composition during maturing and spawning of the sponge dysidea herbacea (Porifera: Demospongiae). Comp. Biochem. Physiol. 1997; 118B: 851–859.Google Scholar
- 12.Ministry of Education, Culture, Sports, Science and Technology. Standard tables of food composition in Japan. Ministry of education, culture, sports, science and technology, Fifth Revised and Enlarged Edition. National Printing Bureau, Tokyo. 2005.Google Scholar
- 13.Fujimoto K, Kaneda T. Test for antioxigenic compounds from marine algae and fractionation from Eisena bicyclis and Undaria pinnatifida. Nippon Suisan Gakkaishi 1980; 46: 1125–1130.Google Scholar
- 15.Hirata T, Iida H, Tanaka M, Ooike M, Tsunomura T, Sakaguchi M. Bio-regulatory functions of biliproteins and phycobilins from algae. Proceedings of International Commemorative Symposium. 70th Anniversary of the Japanese Society of Fisheries Science. Japanese Society of Fisheries Science, Yokohama. 2002; 1449–1452.Google Scholar
- 16.Figueroa FL, Escassi L, Perez-Rodriguez E, Korbee N, Giles AD, Johnsen G. Effects of short-term irradiation on photo-inhibition and accumulation of mycosporine-like amino acids in sun and shade species of the red algal genus Porphyra. J. Photochem. Photobiol. B Biol. 2003; 69: 21–30.CrossRefGoogle Scholar
- 19.Niki E, Saito T, Kawakami A, Kamiya Y. Inhibition of oxidation of methyl linoleate in solution by Vitamin E and Vitamin C. J. Biol. Chem. 1984; 295: 4177–4182.Google Scholar
- 22.Hirata T, Tanaka M, Ooike M, Tsunomura T, Niizeki N, Sakaguchi M. Radical scavenging activity of phycocyanobilin prepared from the cyanobacterium. Spirulina platensis. Fish. Sci. 1999; 65: 971–972.Google Scholar