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Fisheries Science

, Volume 74, Issue 2, pp 341–346 | Cite as

Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii

  • Seiko Nonaka
  • Tomonori SomamotoEmail author
  • Yoko Kato-Unoki
  • Mitsuru Ototake
  • Teruyuki Nakanishi
  • Miki Nakao
Article

Abstract

The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii, a naturally occurring gynogenetic fish, is a useful model for studying T-cell-mediated immunity. CD4, a T-cell receptor (TCR) coreceptor, is a membrane-bound glycoprotein found on helper T-cells, and assists in the binding of major histocompatibility complexes. In the present study, full-length cDNAs encoding the CD4 molecule from the S3n strain of ginbuna crucian carp were cloned and characterized. 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE yielded two distinct cDNA clones of CD4 homolog from the ginbuna, and these sequences share 95% identity at the amino acid level. These ginbuna CD4 molecules consisted of a signal peptide, immunoglobulin superfamily (lgSf) like domains, a transmembrane domain, and a cytoplasmic domain similar to other known CD4. A tyrosine protein kinase p56Ick binding motif is conserved in the cytoplasmic tail of ginbuna CD4. Phylogenetic tree analysis indicated that ginbuna CD4 sequences are closely related to CD4L-1 from other fish species. Expression of ginbuna CD4 mRNA was detected in the gill, thymus, head kidney, trunk kidney and peripheral blood leukocytes, indicating that its expression pattern is similar to that of ginbuna TCRβ mRNA. The results suggest that ginbuna CD4 sequences are useful as molecular probes for helper T-cells.

Key Words

CD4 cell-mediated immunity ginbuna crucian carp T-cell 

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References

  1. 1.
    Suetake H, Saha NR, Araki K, Akatsu K, Kikuchi K, Suzuki Y. Lymphocyte surface marker genes in fugu. Comp. Biochem. Physiol. Part D 2006; 1: 102–108.Google Scholar
  2. 2.
    Nakanishi T, Fischer U, Dijkstra JM, Hasegawa S, Somamoto T, Okamoto N, Ototake M. Cytotoxic T cell function in fish. Dev. Comp. Immunol. 2002; 26: 131–139.PubMedCrossRefGoogle Scholar
  3. 3.
    Nakanishi T, Okamoto N. Cell-mediated cytotoxicity in isogeneic ginbuna crucian carp. Fish Shellfish Immunol. 1999; 9: 259–267.CrossRefGoogle Scholar
  4. 4.
    Fischer U, Utke K, Somamoto T, Köllner B, Ototake M, Nakanishi T. Cytotoxic activities of fish leucocytes. Fish Shellfish Immunol. 2006; 20: 209–226.PubMedCrossRefGoogle Scholar
  5. 5.
    Somamoto T, Yoshiura Y, Nakanishi T, Ototake M. Molecular cloning and characterization of two types of CD8alpha from ginbuna crucian carp, Carassius auratus langsdorfii. Dev. Comp. Immunol. 2005; 29: 693–702.PubMedCrossRefGoogle Scholar
  6. 6.
    Somamoto T, Yoshiura Y, Sato A, Nakao M, Nakanishi T, Okamoto N, Ototake M. Expression profiles of TCRbeta and CD8alpha mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. Virology 2006; 348: 370–377.PubMedCrossRefGoogle Scholar
  7. 7.
    Weiss A, Samelson LE. T-lymphocyte activation. In: Paul WE (ed.) Fundamental Immunology, 5th edn. Lippincott Williams & Williams, Philadelphia, PA. 2003; 321–363.Google Scholar
  8. 8.
    Brenner S, Venkatesh B, Yap WH, Chou CF, Tay A, Ponniah S, Wang Y, Tan YH. Conserved regulation of the lymphocyte-specific expression of lck in Fugu and mammals. Proc. Natl. Acad. Sci. U. S. A. 2002; 99: 2936–2941.PubMedCrossRefGoogle Scholar
  9. 9.
    Langenau DM, Ferrando AA, Traver D, Kutok JL, Hezel JPD, Kanki JP, Zon LI, Look AT, Trede NS. In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish. Proc. Natl. Acad. Sci. U. S. A. 2004; 101: 7369–7374.PubMedCrossRefGoogle Scholar
  10. 10.
    Araki K, Hirano K, Takizawa F, Moritomo T, Ototake M, Nakanishi T. Identification and characterization of multiple Lck genes in ginbuna crucian carp Carassius auratus langsdorfii. Fish. Sci. 2007; 73: 1017–1024.CrossRefGoogle Scholar
  11. 11.
    Suetake H, Araki K, Suzuki Y. Cloning, expression, and characterization of fugu CD4, the first ectothermic animal CD4. Immunogenetics 2004; 56: 368–374.PubMedCrossRefGoogle Scholar
  12. 12.
    Dijkstra JM, Somamoto T, Moore L, Hordvik I, Ototake M, Fischer U. Identification and characterization of a second CD4-like gene in teleost fish. Mol. Immunol. 2006; 43: 410–419.PubMedCrossRefGoogle Scholar
  13. 13.
    Edholm ES, Stafford JL, Quiniou SM, Waldbieser G, Miller NW, Bengten E, Wilson M. Channel catfish, Ictalurus punctatus, CD4-like molecules. Dev. Comp. Immunol. 2007; 31: 172–187.PubMedCrossRefGoogle Scholar
  14. 14.
    Laing KJ, Zou JJ, Purcell MK, Phillips R, Secombes CJ, Hansen JD. Evolution of the CD4 family: teleost fish possess two divergent forms of CD4 in addition to lymphocyte activation gene-3. J. Immunol. 2006; 177: 3939–3951.PubMedGoogle Scholar
  15. 15.
    Pancer Z, Mayer WE, Klein J, Cooper MD. Prototypic T cell receptor and CD4-like coreceptor are expressed by lymphocytes in the agnathan sea lamprey. Proc. Natl. Acad. Sci. U.S.A. 2004; 101: 13273–13278.PubMedCrossRefGoogle Scholar
  16. 16.
    Stuge TB, Wilson MR, Zhou H, Barker KS, Bengten E, Chinchar G, Miller NW, Clem LW. Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish. J. Immunol. 2000; 164: 2971–2977.PubMedGoogle Scholar

Copyright information

© The Japanese Society of Fisheries Science 2008

Authors and Affiliations

  • Seiko Nonaka
    • 1
  • Tomonori Somamoto
    • 1
    Email author
  • Yoko Kato-Unoki
    • 1
  • Mitsuru Ototake
    • 2
  • Teruyuki Nakanishi
    • 3
  • Miki Nakao
    • 1
  1. 1.Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  2. 2.Inland StationNational Research Institute of Aquaculture, FRAMieJapan
  3. 3.Department of Veterinary Medicine, College of Bioresource SciencesNihon UniversityFujisawa, KanagawaJapan

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