Fisheries Science

, Volume 73, Issue 5, pp 1087–1093

Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers

  • Sang -Gyu Kim
  • Kagayaki Morishima
  • Nobukazu Satoh
  • Takashi Fujioka
  • Setsuo Saito
  • Katsutoshi Arai
Article

DOI: 10.1111/j.1444-2906.2007.01440.x

Cite this article as:
Kim, S.G., Morishima, K., Satoh, N. et al. Fish Sci (2007) 73: 1087. doi:10.1111/j.1444-2906.2007.01440.x

Abstract

Five loci (Phz2, Phz6, Phz7, Phz12, and Phz14) of microsatellite DNA markers developed in a previous study for parentage assignment in the hatchery population generated by mating among 61 broodstock fish (35 females and 26 males) in a spawning tank, were selected. After natural spawning in the same tank, larvae collected at three different times were categorized into early phase (EP), middle phase (MP), and late phase (LP) groups. In the parental broodstock, the mean number of alleles per locus was 21.8 and expected heterozygosity (HE) was 0.813. In the progeny, the mean number of alleles per locus decreased to 11.6 (EP), 14.4 (MP), and 6.4 (LP) and HE to 0.796 (EP), 0.833 (MP), and 0.681 (LP). Parental assignment determined eight dams and six sires as major parents for the EP group. In the MP group, 13 dams and ten sires genetically contributed to spawning, but only three dams and two sires were involved in LP group progeny. In the hatchery population produced from a limited number of parental fish such as the LP group, genetic variability was apparently decreased.

Key Words

effective population size genetic diversity hatchery population heterozygosity microsatellite DNA parentage assignment Pleuronectes herzensteini 

Copyright information

© The Japanese Society of Fisheries Science 2007

Authors and Affiliations

  • Sang -Gyu Kim
    • 1
  • Kagayaki Morishima
    • 1
  • Nobukazu Satoh
    • 2
  • Takashi Fujioka
    • 2
  • Setsuo Saito
    • 2
  • Katsutoshi Arai
    • 1
  1. 1.Graduate School of Fisheries SciencesHokkaido UniversityHakodate, HokkaidoJapan
  2. 2.Hokkaido Institute of MaricultureShikabe, HokkaidoJapan
  3. 3.Korea Inter-University Institute of Ocean SciencePukyong National UniversityBusanKorea
  4. 4.Fisheries LaboratoryKinki UniversityWakayamaJapan
  5. 5.Hokkaido Mariculture Fisheries Experiment StationMuroran, HokkaidoJapan
  6. 6.Hokkaido Hakodate Fisheries Experiment StationHakodate, HokkaidoJapan

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