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Fisheries Science

, Volume 73, Issue 4, pp 871–880 | Cite as

Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species

  • Ryoma Kamikawa
  • Isao Masuda
  • Kenichi Oyama
  • Sadaaki Yoshimatsu
  • Yoshihiko Sako
Article

Abstract

In this study, nuclear ribosomal RNA gene internal transcribed spacer regions and the cox2-cox1 fragment of the mitochondrial (mt) genome were sequenced in 24 strains of Chattonella spp. Variability in both regions showed that the mt genome sequences of Chattonella spp. have a higher evolutionary rate than the nuclear rRNA gene sequences. A maximum likelihood tree based on the mt sequence grouped the Japanese Chattonella strains into two groups (Groups A and B), although no correlation was observed amongst the phylogenetic groups, their morphologies, or the isolated areas. Groups A and B were clearly identified by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay using Fokl, without the need for a sequencing experiment. The PCR-RFLP assay revealed that Chattonella cells obtained from sea water in Oita, Japan, in 2004 and 2005 belonged to Group B. This is the first report showing the genetic variation in Chattonella spp. using a PCR-RFLP identification protocol.

Key words

Chattonella spp. cytochrome c oxidase subunit 1 (cox1cytochrome c oxidase subunit 2 (cox2genetic diversity harmful algae mitochondria 

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Copyright information

© The Japanese Society of Fisheries Science 2007

Authors and Affiliations

  • Ryoma Kamikawa
    • 1
  • Isao Masuda
    • 1
  • Kenichi Oyama
    • 2
  • Sadaaki Yoshimatsu
    • 2
  • Yoshihiko Sako
    • 1
  1. 1.Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Akashiwo Research Institute of Kagawa PrefectureTakamatsu, KagawaJapan

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