Fisheries Science

, Volume 71, Issue 5, pp 1036–1041 | Cite as

The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds



The effectiveness on water quality, population density of bacteria, and shrimp production in ponds treated with commercial probiotics was tested in Penaeus vannamei ponds in Hai-yan, China. Six ponds with replicates for treatment and control were used. Results showed that the probiotics could improve the population density of beneficial bacterial flora, reduce concentrations of nitrogen and phosphorus, and increase yields of shrimp. The average counts of Bacillus sp., ammonifying bacteria, and protein mineralizing bacteria were found to be significantly higher in treated ponds compared to control ponds (P < 0.05). In control ponds, an increase in presumptive vibrios was observed and the average density was up to 2.09 × 103 cfu/mL, whereas that was only 4.37 × 102 cfu/mL in treated ponds (P < 0.05). The use of probiotics also significantly increased dissolved oxygen (P < 0.05) and reduced dissolved reactive-phosphorus, total inorganic nitrogen and chemical oxygen demand (P < 0.05). An average of 8215±265 kg shrimp/ha was obtained in treated ponds with a feed conversion ratio (FCR) of 1.13±0.05 and survival rate of 81.00±6.25% compared with 4985±503 kg shrimp/ha, 1.35±0.12 and 48.67±3.51%, respectively, in control ponds. This indicates that the addition of the commercial probiotics had a noticeable influence on water quality of shrimp ponds and shrimp production.

Key words

bacterial population Penaeus vannamei probiotics water quality 


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  1. 1.
    Lightner DV, Redman RM. Shrimp diseases and current diagnostic methods. Aquaculture 1998; 164: 201–230.CrossRefGoogle Scholar
  2. 2.
    Boyd CE. Chemistry and efficacy of amendments used to treat water and soil quality imbalances in shrimp ponds. In: Browdy CL, Hopkins JS (eds). Proceedings of the Special Session on Shrimp Farming. The World Aquaculture Society, Baton Rouge, LA. 1995: 183–189.Google Scholar
  3. 3.
    Boyd CE, Tucker CS. Pond Aquaculture Water Quality Management. Kluwer, Norwell, MA. 1998.Google Scholar
  4. 4.
    Kozasa M. Toyocerin. (Bacillus toyoi) as growth promotor for animal feeding. Microbiol. Aliment. Nutrition 1986; 4: 121–135.Google Scholar
  5. 5.
    Porubcan RS. Reduction in chemical oxygen demand and improvement in Penaeus monodon yield in ponds inoculated with aerobic Bacillus bacteria. Program and Abstract of the 22nd Annual Conference and Exposition of the World Aquaculture Socity. World Aquaculture Society, San Juan. 1991; 16–20.Google Scholar
  6. 6.
    Gatesoupe FJ. Further advance in the nutritional and antibacterial treatments of rotifers as food for turbot larvae, Scophthalmus maximus L. In: De Pauw N, Jaspers E, Ackefors H, Wilkins N (eds). Aquaculture-a Biotechnology in Progress. European Aquaculture Society, Bredene. 1989; 721–730.Google Scholar
  7. 7.
    Gatesoupe FJ. The effect of three strains of lactic bacteria on the production rate of rotifers, Brachion us plicatilis, and their dietary value for larval turbot, Scophthalmus maximus. Aquaculture 1991; 96: 335–342.CrossRefGoogle Scholar
  8. 8.
    Noh SH, Han K, Won TH, Choi YJ. Effect of antibiotics, enzyme, yeast culture and probiotics on the growth performence of Israeli carp. Korean J. Anim. Sci. 1994; 36: 480–486.Google Scholar
  9. 9.
    Bogut I, Milakovic Z, Bukvic Z, Brkic S, Zimmer R. Influence of probiotic (Streptococcus faecium M74) on growth and content of intestinal microflora in carp (Cyprinus carpio). Czech J. Anim. Sci. 1998; 43: 231–235.Google Scholar
  10. 10.
    Gournier-Chateau N, Larpent JP, Castellanos I, Larpent JL. Les Probiotiques en Alimentation Animale et Humain. Technique et Documentation Lavoisier, Paris. 1994.Google Scholar
  11. 11.
    Chuntapa B, Powtongsook S, Menasveta P. Water quality control using Spirulina platensis in shrimp culture tanks. Aquaculture 2003; 220: 355–366.CrossRefGoogle Scholar
  12. 12.
    Shariff M, Yusoff FM, Devaraja TN, Srinivasa Rao PS. The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius), ponds. Aquacult Res. 2001; 32: 181–187.CrossRefGoogle Scholar
  13. 13.
    Dong XZ, Cai MZ. Common Manual of Determinative Bacteriology. Science Press, Beijing. 2001.Google Scholar
  14. 14.
    Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology, 9th edn. Williams & Wilkins, Baltimore. 1994.Google Scholar
  15. 15.
    Wei FS. Method on Water and Waste Water Analysis. Environmental. Science of China Press, Beijing. 2002.Google Scholar
  16. 16.
    Parsons TR, Maita Y, Lalli CM. A Manual of Chemical and Physical Methods for Sea Water Analysis. Pergamon Press Ltd, Oxford. 1984.Google Scholar
  17. 17.
    Gilliland SE, Busta FF, Brinda JJ, Campbell JE. Aerobic plate count. In: Speck ML (ed.). Compendium of Methods for the Microbiological Examination of Foods. American Public Health Association, Washington. 1976; 107–130.Google Scholar
  18. 18.
    Austin B. Methods in Aquatic Bacteriology. Wiley Press, New York. 1988.Google Scholar
  19. 19.
    Sepers ABJ. Diversity of ammonifying bacteria. Hydrobiologia 1981; 83: 343–350.CrossRefGoogle Scholar
  20. 20.
    Rodina AG. Methods in Aquatic Microbiology. Colwell RR, Zambruski MS (Trans). University Park Press, Baltimore. 1972; 461.Google Scholar
  21. 21.
    Ming DX. Biometry and Experimental Design. Agriculture of China Press, Version 3, Beijing. 2002.Google Scholar
  22. 22.
    Prayitno SB, Latchford JW. Experimental infections of crustaceans with luminous bacteria related to Photobacterium and Vibrio. Effect of salinity and pH on infectiosity. Aquaculture 1995; 132: 105–112.CrossRefGoogle Scholar
  23. 23.
    Lavilla-Pitogo CR, Baticados MCL, Cruz-Lacierda ER, de la Pena LD. Occurrence of luminous bacterial diseases of Penaeus monodon larvae in the Philippines. Aquaculture 1990; 91: 1–13.CrossRefGoogle Scholar
  24. 24.
    Chanratchakool P, Pearson M, Limsuwan C, Roberts RJ. Oxytetracycline sensitivity of Vibrio species isolated from diseased black tiger shrimp. Penaeus monodon Fabricius. J. Fish Dis. 1995; 18: 79–82.CrossRefGoogle Scholar
  25. 25.
    Xu B, Xu HS Ji WS, Shi J. Pathogens and pathogenicity to Penaeus orientalis Kishinouye. Acta Oceanologica Sinica 1994; 13 (2): 297–304.Google Scholar
  26. 26.
    Nash G, Nithimathachoke C, Tungmandi C, Arkarjamorn A, Prathanpipat P, Ruamthaveesub P. Vibriosis and its control in pond-reared Penaeus monodon in Thailand. In: Shariff M, Subasinghe RP, Arthur JR (eds). Diseases in Asian Aquaculture I. Fish Health Section. Asian Fisheries Society, Manila. 1992; 143–155.Google Scholar
  27. 27.
    Thomas GM, Ward CH, Raymond RL, Wilson JT, Loehr RC. Bioremediation. In: Leperberg J (ed.). Encyclopedia of Microbiology, Vol 1. Academic Press, New York. 1992; 369–385.Google Scholar
  28. 28.
    Yang CH, Huang J. The Harmless and Healthy Technology of Shrimp Culture. Agriculture of China Press, Beijing. 2003.Google Scholar
  29. 29.
    Boyd CE, Fast AW. Fond monitoring and management. In: Fast AW, Lester JL (eds). Marine Shrimp Culture-Principles and Practices. Elsevier, Amsterdam. 1992; 497–513.Google Scholar
  30. 30.
    Hepher B. Primary production in fishponds and its application to fertilization experiments. Limno. Oceanogr. 1962; 7: 131–135.CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Fisheries Science 2005

Authors and Affiliations

  1. 1.The Key Laboratory of Molecular Animal Nutrition, Ministry of EducationFeed Science Institute of Zhejiang UniversityHangzhouChina

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