Fisheries Science

, Volume 71, Issue 2, pp 327–332 | Cite as

Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel

  • Haruhiko Toyohara
  • Masatomi Hosoi
  • Isao Hayashi
  • Satoshi Kubota
  • Hisashi Hashimoto
  • Yoshihiro Yokoyama


Expression of HSP70 in response to heat-shock was investigated at the protein and mRNA levels in Mediterranean blue mussel. Western and Northern blot analyses revealed that HSP70 was expressed following heat-shock in the mantle at both protein and mRNA levels, suggesting that gene expression of HSP70 is implicated in the cellular response to heat-shock stress in mussel. It was then attempted to clone HSP70 cDNA in order to determine the primary structure of mussel HSP70. As a result, two full-length cDNA encoding HSP70 were isolated from a cDNA library prepared from the heat-shocked mantle. The isolated cDNA consist of single open reading frames of 2067 bp and 1911 bp which encode proteins of 689 amino acids and 637 amino acids, respectively. Both HSP70 cDNA encode an ATPase do main, and a substrate-binding do main in addition to a Glu-Glu-Val-Asp (EEVD) peptide motif that is specific for cytosolic HSP70. These findings suggest that the cDNA clones obtained in the present study encode cytosolic HSP70.

Key Words

heat-shock HSP70 mantle Mediterranean blue mussel Mytilus galloprovin-cialis stress 


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Copyright information

© The Japanese Society of Fisheries Science 2005

Authors and Affiliations

  • Haruhiko Toyohara
    • 1
  • Masatomi Hosoi
    • 1
  • Isao Hayashi
    • 1
  • Satoshi Kubota
    • 2
  • Hisashi Hashimoto
    • 3
  • Yoshihiro Yokoyama
    • 4
  1. 1.Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Division of Human Health and Medical Science, Graduate School of Kuroshio ScienceKochi UniversityNankokuJapan
  3. 3.Bioscience and Biotechnology CenterNagoya UniversityNagoyaJapan
  4. 4.Department of Marine BioscienceFukui Prefectural UniversityFukuiJapan

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