Abstract
In this study, the authors examined the role of bovine herpesvirus type 1 (BHV-1) Us9 in the anterograde transport of the virus from trigeminal ganglia (TG) to nose and eye upon reactivation from latency. During primary infection, both BHV-1 Us9-deleted and BHV-1 Us9-rescued viruses replicated efficiently in the nasal and ocular epithelium. However, upon reactivation from latency, only the BHV-1 Us9-rescued virus could be isolated in the nasal and ocular shedding. By real-time polymerase chain reaction, comparable DNA copy numbers were detected in the TGs during latency and reactivation for both the viruses. Therefore, Us9 is essential for reactivation of the virus in the TG and anterograde axonal transport from TG to nose and eye.
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References
Antinone SE, Smith GA (2006). Two modes of herpesvirus trafficking in neurons: membrane acquisition directs motion. J Virol 80: 11235–11240.
Belknap EB, Collins JK, Ayers VK, Schulthesis PC (1994). Experimental infection of neonatal calves with neurovirulent bovine herpes virus type 1.3. Vet Pathol 31: 358–365.
Brideau AD, Card JP, Enquist LW (2000). Role of pseudorabies virus Us9, a type II membrane protein, in infection of tissue culture cells and the rat neuron system. J Virol 74: 834–845.
Ch’ng TH, Enquist LW (2005). Efficient axonal localization of alpha herpes virus structural proteins in cultures sympathetic neurons requires viral glycoprotein E. J Virol 79: 8835–8846.
Chowdhury SI, Lee BJ, Mosier D, Sur J-H, Osario FA, Kennedy G, Weiss ML (1997). Neuropathology of bovine herpesvirus type 5 (BHV-5) meningo-encephalitis in a rabbit seizure model. J Comp. Pathol 117: 295–310.
Chowdhury SI, Lee BJ, Ozkul A, Weiss ML (2000). Bovine herpesvirus 5 glycoprotein E is important for neuroinvasiveness and neurovirulence in the olfactory pathway of the rabbit. J Virol 74: 2094–2106.
Chowdhury SI, Ludwig H, Buhk HJ (1988). Molecular biological characterization of equine herpesvirus 1 (EHV-1) isolated from ruminant hosts. Virus Res 11: 127–139.
Chowdhury SI, Mahmood S, Simon J, Al-Mubarak A, Zhou Y (2006). The Us9 gene carried by Bovine Herpesvirus 1 (BHV-1) effectively complements a Us9-null strain of BHV-5 for anterograde transport, neurovirulence and neuro-invasiveness in a rabbit model. J Virol 80: 4396–405.
Chowdhury SI, Onderci M, Bhattacharjee PS, Al-Mubarak A, Weiss ML, Zhou Y (2002). Bovine herpesvirus 5 (BHV-5) Us9 is essential for BHV-5 neuropathogenesis. J Virol 76: 3839–3851.
Enquist LW (2002). Exploiting circuit specific spread of pseudorabies virus in the central nervous system: insights to pathogenesis and circuit tracers. J Infect. Dis 186: 209–214.
Inman M, Lovato L, Doster A, Jones C (2002). A mutation in the latency-related gene of bovine herpesvirus 1disrupts the latency reactivation cycle in calves. 76: 6771–6779.
Jiang Y, Hossain A, Winkler MT, Holt T, Doster A, Jones C (1998). A protein encoded by the latency related gene of bovine herpes virus 1 is expressed in trigeminal ganglionic neurons of latently infected cattle and interacts with cyclin-dependent kinase 2 during productive infection. J Virol 72: 8133–8142.
Jones C (1998). Alpha herpes virus latency: its role in disease and survival of the virus in nature. Adv. Virus Res 51: 81–133.
Jones C, Newby TJ, Holt T, Doster A, Stone M, Ciacci-Zanella J, Webster CJ, Jackwood MW (2000). Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpesvirus 1 (RLB106). Vaccine 18: 3185–3195. J Virol 74: 4776–4786.
Kutish G, Mainprize T, Rock D (1990). Characterization of the latency-related transcriptionally active region of the bovine herpesvirus 1 genome. J Virol 64: 5730–5737.
Lee BJ, Weiss ML, Mosier D, Chowdhury SI (1999). Spread of bovine herpesvirus type5 (BHV-5) in the rabit brain after intranasal inoculation. J NeuroVirol 5: 473–483.
Rock D, Lokensgard J, Lewis T, Kutish G (1992). Characterization of dexamethasone-induced reactivation of latent bovine herpes virus-1. J Virol 66: 2484–2490.
Tikko SK, Campos M, Babiuk LA (1995). Bovine herpes virus 1 (BHV-1): biology, pathogenesis, and control. Adv. Virus Res 45: 191–223.
Tomishima MJ, Enquist LW (2001). A conserved α-herpes virus protein necessary for axonal localization of viral membrane proteins. J Cell Biol 154: 741–752.
Tomishima MJ, Smith G, Enquist LW (2001). Sorting and transport of alpha herpes viruses in axons. Traffic 2: 429–436.
Wang F, Tang W, McGraw HM, Bennet J, Enquist LW, Friedman HM (2005). Herpes simplex virus type 1 glycoprotein E is required for axonal localization of capsid, tegument, and membrane glycoproteins. J Virol 79: 13362–13372.
Winkler MT, Doster A, Sur JH, Jones C (2002). Analysis of bovine trigeminal ganglia following infection with bovine herpes virus-1. Vet Microbiol 86: 139–155.
Wyler R, Engeis M, Schwyzer M (1989). Infectious bovine rhino-tracheitis/vulvovaginitis (BHV-1). In Herpes virus diseases of cattle, horses and pigs. G. Wittman (ed). Hinhman, MA: Kluwer Academic Publishers, pp 1–72.
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This work is published as contribution # 07-91-J of the Kansas Agricultural Experiment Station.
This work was supported by USDA grants to S. I. Chowdhury (00-02103 and 2004-35204-14657) and C. Jones (2005-01554, 2006-01627).
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Butchi, N.B., Jones, C., Perez, S. et al. Envelope protein Us9 is required for the anterograde transport of bovine herpesvirus type 1 from trigeminal ganglia to nose and eye upon reactivation. Journal of NeuroVirology 13, 384–388 (2007). https://doi.org/10.1080/13550280701375433
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DOI: https://doi.org/10.1080/13550280701375433