Journal of NeuroVirology

, Volume 12, Issue 2, pp 146–152

Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

  • Melissa J. Churchill
  • Paul R. Gorry
  • Daniel Cowley
  • Luxshimi Lal
  • Secondo Sonza
  • Damian F. J. Purcell
  • Katherine A. Thompson
  • Dana Gabuzda
  • Justin C. McArthur
  • Carlos A. Pardo
  • Steven L. Wesselingh
Short Communication

Abstract

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

Keywords

astrocyte HIV-1 HIVD HIVE macrophage provirus 

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Copyright information

© Journal of NeuroVirology, Inc. 2006

Authors and Affiliations

  • Melissa J. Churchill
    • 1
  • Paul R. Gorry
    • 1
    • 2
    • 4
  • Daniel Cowley
    • 1
  • Luxshimi Lal
    • 1
  • Secondo Sonza
    • 1
    • 3
  • Damian F. J. Purcell
    • 4
  • Katherine A. Thompson
    • 1
  • Dana Gabuzda
    • 5
    • 6
  • Justin C. McArthur
    • 7
  • Carlos A. Pardo
    • 7
  • Steven L. Wesselingh
    • 1
    • 2
  1. 1.The Macfarlane Burnet Institute for Medical Research and Public HealthMelbourneAustralia
  2. 2.Department of MedicineMonash UniversityMelbourneAustralia
  3. 3.Department of MicrobiologyMonash UniversityMelbourneAustralia
  4. 4.Department of Microbiology and ImmunologyUniversity of MelbourneMelbourneAustralia
  5. 5.Department of Cancer Immunology and AIDSDana-Farber Cancer InstituteBostonUSA
  6. 6.Department of NeurologyHarvard Medical SchoolBostonUSA
  7. 7.Department of NeurologyJohns Hopkins University School of MedicineBaltimoreUSA

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