Journal of NeuroVirology

, Volume 7, Issue 5, pp 432–436 | Cite as

Sequence analysis of the S gene of recombinant MHV-2/A59 coronaviruses reveals three candidate mutations associated with demyelination and hepatitis

  • Jayasri Das Sarma
  • Li Fu
  • Susan T. Hingley
  • Michael M. C. Lai
  • Ehud Lavi
Article

Abstract

Coronaviruses, mouse hepatitis virus (MHV) strains, exhibit various degrees of neurotropism and hepatotropism following intracerebral (IC) infection of 4-week-old C57Bl/6 mice. Whereas MHV-A59 produces acute meningitis, encephalitis, hepatitis, and chronic demyelination, a closely related strain, MHV-2, produces only acute meningitis and hepatitis. We previously reported that the spike glycoprotein gene of MHV contains determinants of demyelination and hepatitis. To further investigate the site of demyelination and hepatitis determinants within the S gene, we sequenced the S gene of several nonde-myelinating recombinant viruses. We found that three encephalitis-positive, demyelination-negative, hepatitis-negative recombinant viruses have an MHV-A59-derived S gene, which contains three identical point mutations (I375M, L652I, and T1087N). One or more of the sites of these mutations in the MHV-A59 genome are likely to contribute to demyelination and hepatitis.

Keywords

coronavirus nidoviruses mouse hepatitis virus (MHN) demyelination hepatitis multiple sclerosis (MS) 

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Copyright information

© Journal of NeuroVirology, Inc. 2001

Authors and Affiliations

  • Jayasri Das Sarma
    • 1
  • Li Fu
    • 1
  • Susan T. Hingley
    • 2
  • Michael M. C. Lai
    • 3
  • Ehud Lavi
    • 1
  1. 1.Department of Pathology and Laboratory Medicine, 613 Stellar-Chance LaboratoriesUniversity of Pennsylvania, School of Medicine, Division of NeuropathologyPhiladelphiaUSA
  2. 2.Department of Microbiology and ImmunologyPhiladelphia College of Osteopathic MedicinePhiladelphia
  3. 3.Department of Molecular Microbiology and Immunology, Howard Hughes Medical InstituteUniversity of Southern California, School of MedicineLos AngelesUSA

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