In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 5, pp 394–398 | Cite as

High-frequency induction of multiple shoots and clonal propagation from rhizomatous nodal segments of Houttuynia cordata Thunb.—An ethnomedicinal herb of India

  • Saswati Chakraborti
  • Sangram Sinha
  • Rabindra K. SinhaEmail author


This study reports an efficient and direct shoot bud differentiation and multiple shoot induction from nodal segments of underground stoloniferous rhizomes of Houttuynia cordata Thumb. The frequency of shoot bud regeneration was influenced by the type of cytokinin and concentrations. Among the various concentrations used, benzylaminopurine (BAP, 17.74 μM) or kinetin (Kn, 18.58 μM) was found to be most effective for rapid and maximum shoot but differentiation. The number of shoots per explant was higher (20.00±2.61) on Murashige and Skoog (MS) medium supplemented with Kn (18.58 μM) compared to BAP and 6-γ-γ-(dimethyl-allylamino)-purine (2iP) during initial 40-d-old culture. Subsequent shoot differentiation and multiplication were achieved in MS medium containing 9.29 μM Kn and 15% (v/v) coconut milk. Elongation and growth of multiple shoots were also obtained on MS medium containing either 2.32 μM Kn or 2.46 μM 2iP alone. The rate of shoot multiplication during subcultures declined with an increase in the size of proliferating shoot cluster. Reducing shoot cluster size to three to four shoots and subculturing together in shoot multiplication medium resulted in a better shoot multiplication and growth, which could be maintained for 2 yr. The elongated shoots (>20 mm) were successfully rooted on MS medium supplemented with 19.60 μM indole-3-butyric acid. Regenerated plants were successfully established in soil and were found to be healthy and uniform. The protocol reported in this study can be used for conservation and utilization of elite clone of H. cordata.

Key words

ethnomedicinal herb Houttuynia cordata micropropagation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aitken-Christie, J.; Connell, M. Clonal propagation; gymosperms. In: Vasil, I. K., ed. Cell culture and somatic cell genetics of plants. New York: Academic Press; 1992:82–95.Google Scholar
  2. Borthakur, M.; Singh, R. S.; Bora, P. In vitro regeneration of Houttuynia corlats: a medicinal herb Planta Med. 65:677; 1999.PubMedCrossRefGoogle Scholar
  3. Brown, D. Encyclopedia of herbs and their uses. London: Dorling Kindersley; 1995.Google Scholar
  4. Dev, D.B. The flora of Tripura, vol. II. New Delhi. Today and Tomorrow's Publishers; 1983:139–140.Google Scholar
  5. Edson, J. L.; Wenny, D. L.; Leege-Brusven, A. D.; Everell, R. L. Using micropropagation to conserve threatened rare species in sustainable forests. J. Sust. For. 5:279–291; 1997.CrossRefGoogle Scholar
  6. Hall, R. D. Plant cell culture initiation: practical tips. Mol. Biotechnol. 16:161–173; 2000.PubMedCrossRefGoogle Scholar
  7. Handique, P. J.; Bora, P. In vitro regeneration of a medicinal plant—Houttuynia cordata Thunb. from nodal explants. Curr. Sci. 76:1245–1247; 1999.Google Scholar
  8. Kanjilal, P. C.; Dev, R. N. Flora of Assam, vol. III New Delhi: Omsons Publishers; 1937:113.Google Scholar
  9. Kyoko, H.; Kamiya, M.; Hayashi, T. Viricidal effects of the steam distillate from Houttuynia cordata Thunb. and its components on HSV-1, influenza virus and HIV. Planta Med. 61:237–241; 1994.Google Scholar
  10. Mukherjee, A.; Roy, S. D. An account of Piperaceae and Saururaceae in the hills of Darjeeling district, West Bengal (India). J. Econ. Tax. Bot. 9:367–372; 1987.Google Scholar
  11. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.CrossRefGoogle Scholar
  12. Park, J. C.; Hur, J. M.; Park, S. J.; Lee, J. H.; Sung, N. J.; Choi, M. R.; Song, S. H.; Kim, M. S.; Choi, J. W. The effects of Houttuynia cordata on the hepatic bromobenzene metabolizing enzyme system in rats and isolation of phenolic compounds. Kor. J. Pharmacol. 31:228–234; 2000.Google Scholar
  13. Preece, J. E.; Imel, M. R. Plant regeneration from leaf explants of rhododendron ‘P. J. M. hybrids. Sci. Hort. 48:159–170; 1991.CrossRefGoogle Scholar
  14. Rani, G.; Virk, G. S.; Nagpal, A. Callus induction and plantlet regeneration in Withania somnifera (L.) Dunal. In vitro Cell. Dev. Biol.—Plant 39:468–474; 2003.CrossRefGoogle Scholar
  15. Rastogi, R. P.; Mehrotra, B. N. Compendum of Indian medicinal plants, vol. 2. New Delhi: Lucknow and Information Directorate; 1993:376.Google Scholar
  16. Saha, S.; Mukhopadhyay, M.; Mukhopadhyay, S. In vitro clonal propagation through bud culture of Hemidesmus indicus (L) R Br: an important medicinal herb. J. Plant Biochem. Biotechnol. 12:61–64; 2003.Google Scholar
  17. Sinha, R. K.; Chakraborti, S.; Roy, K.; Sinha, S. Traditional knowledge on some ethnobotanical species of Tripura—its utility and future prospects. In: International Conference on ‘promotion and development of botanicals with international co-ordination: exploring quality, safety, efficacy and regulations, Kolkata, India; 2005:63.Google Scholar
  18. Sinha, R. K.; Mallick, R. Plantlets from somatic callus tissue of the woody legume Sesbania bispinosa (Jacq.) W. F. Wight. Plant Cell Rep. 10:247–250; 1991.CrossRefGoogle Scholar
  19. Sugiura, A.; Tao, R.; Murayama, H.; Tomana, T. In vitro propagation of Japanese persimmon. HortSci. 21:1205–1207; 1986.Google Scholar
  20. Tauzuki, K.; Miyasita, S.; Kono, N. Report on the plant regeneration from the callus made of the leaf segments of Houttuynia cordata Thunb. Bull. Nip. Vet. Anim. Sci. Univ. 44:65–68; 1995.Google Scholar
  21. Torrey, J. G. Root hormones and plant growth. Annu. Rev. Plant Physiol. 27:435–459; 1976.CrossRefGoogle Scholar
  22. Tutupalli, L. V.; Chaubal, M. G.; Saururaceae, V. Composition of essential oil from foliage of Houttuynia cordata and chemosystematics of Saururaceae. Lloydia 38:92–96; 1975.PubMedGoogle Scholar
  23. Wareing, R. F.; Phillips, I. D. J. Growth and differentiation in plants. Oxford: Pergamon Press; 1981.Google Scholar
  24. Winnaar, W. De. Clonal propagation of papaya in vitro. Plant Cell Tiss. Organ Cult. 12:305–310; 1988.CrossRefGoogle Scholar
  25. Wochok, Z. S. The role of tissue culture in preserving threatened and endangered plant species. Biol. Conserv. 20:83–89; 1981.CrossRefGoogle Scholar
  26. Wu, W.; Zheng, Y.; Liu, F.; Tan, G.; Ren, H.; Zhang, W. Construction of fast propagation system of Houttuynia cordata new line. Zhongguo Zhongyao Zazhi 29:27–28; 2004.Google Scholar

Copyright information

© Society for In Vitro Biology 2006

Authors and Affiliations

  • Saswati Chakraborti
    • 1
  • Sangram Sinha
    • 1
  • Rabindra K. Sinha
    • 1
    Email author
  1. 1.Cytogenetics and Plant Tissue Culture Laboratory, Department of Life ScienceTripura UniversityTripuraIndia

Personalised recommendations