Summary
Rooting in vitro was examined for lentil nodal segments to test a recently published conclusion that shoot orientation has an effect on rooting. As is the case for many species, roots initiated and grew only at the proximal end of the microcutting regardless of its orientation. When the proximal end was in agar (a hypoxic environment) the rooting percentage was low (9–25%) even when the orientation of the microcutting was altered by inventing the culture tube. In contrast, when the proximal end of the microcutting was in an aerobic environment (from the shoot being placed upside down in agar medium or placed normally or upside down in an aerated medium), rooting percentages were higher (62–100%). Medium aeration at the proximal end of the microcutting is more important than shoot orientation for in vitro rooting of lentil microcuttings.
Similar content being viewed by others
References
Ahmad, M.; Fautier, A. G.; McNeil, D. L.; Hill, G. D.; Burritt, D. J. In vitro propagation of Lens species and their Fl interspecific hybrids. Plant Cell Tiss. Organ Cult. 47:169–176; 1997.
Bloch, R. Polarity in plants. Bot. Rev. 9:261–310; 1943.
De Klerk, G. J. Rooting of microcuttings: theory and practice. In Vitro Cell. Dev. Biol. Plant 38:415–422; 2002.
Fratini, R.; Ruiz, M. L. Comparative study of different cytokinins in the induction of morphogenesis in lentil (Lens culinaris Medik.). In Vitro Cell. Dev. Biol. Plant 38:46–51; 2002.
Fratini, R.; Ruiz, M. L. A rooting procedure for lentil (Lens culinaris Medik.) and other hypogeous legumes (pea, chickpea and Lathurus) based on explant polarity. Plant Cell Rep. 21:726–732; 2003.
Guan, H.; De Klerk, G. Regeneration of roots from apple stem segements in vitro: effect of orientation on the medium. Acta Hort. 520:171–175; 2000.
Gulati, A.; Schryer, P.; McHughen, A. Regeneration and micrografting of lentil shoots. In Vitro Cell. Dev. Biol. Plant 37:798–802; 2001.
Haissig, B. E. Influences of auxins and auxin synergists on adventitious root primordium initiation and development. NZ J. Sci. 4:311–323; 1974.
Moncousin, C. Rooting of microcuttings: fundamental aspects. Acta Hort. 289:311–317; 1991.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Newell, C.; Growns, D.; McComb, J. A. A novel in vitro rooting method employing aerobic medium with wide application to the Australian flora. Aust. J. Bot. 53:81–89; 2005.
Newell, C.; McComb, J. A.; Growns, D. The influence of medium aeration on in vitro rooting of Australian plant microcuttings. Plant Cell Tiss. Organ Cult. 75:131–142; 2003.
Pierik, R. L. M.; Steegmans, H. H. M. Analysis of adventitious root formation in isolated stem explants of Rhododendron. Sci. Hort. 3:1–20; 1975.
Polanco, M. C.; Pelaez, M. I.; Ruiz, M. L. Factors affecting callus and shoot formation from in vitro cultures of Lens culinaris Medik. Plant Cell Tiss. Organ Cult. 15:175–182; 1988.
Polanco, M. C.; Ruiz, M. L. Effect of benzylaminopurine on in vitro and in vitro root development in lentil Lens culinaris Medik. Plant Cell Rep. 17:22–26; 1997.
Polanco, M. C.; Ruiz, M. L. Factors that affect plant regeneration from in vitro culture of immature seeds in four lentil cultivars. Plant Cell Tiss. Organ Cult. 66:133–139; 2001.
Skoog, F.; Tsui, C. Chemical control of growth and bud formation in tobacco stem segments and callus cultured in vitro. Am. J. Bot. 35:782–787; 1948.
Warmke, H. E.; Warmke, G. L. The role of auxin in the differentiation of root and shoot primordia form root cuttings of Taraxacum and Cichorium. Am. J. Bot. 37:272–280; 1950.
Williams, D. J.; McHughen, A. Plant regenration of the legume Lens culinaris Medik (lentil) in vitro. Plant Cell Tiss. Organ Cult. 7:149–153; 1986.
Zimmerman, R. H.; Fordham, I. Simplified methods for rooting apple cultivars in vitro. J. Am. Soc. Hort. Sci. 110:34–38; 1985.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Newell, C., Growns, D. & McComb, J. Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) cv. ‘Digger” microcuttings in vitro . In Vitro Cell.Dev.Biol.-Plant 42, 197–200 (2006). https://doi.org/10.1079/IVP2006747
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2006747