Summary
This study reports a protocol for high-efficiency plant regeneration from leaf explants of male Himalayan poplar (Populus ciliata Wall.). Shoots were regenerated at high frequencies from explants grown on Murashige and Skoog (MS) medium supplemented with 0.5 mg l−1 kinetin and 0.2 mg l−1 indole-3-acetic acid (IAA). Regenerated shoots developed roots in MS medium supplemented with 0.1 mg l−1 IAA. Himalayan poplar plantlets could be produced within 2 mo. after acclimatization in a sterile mixture of sand and soil.
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References
Belaizi, M.; Paul, H.; Sangwan, R. S.; Sangwan-Norreel, B. S. Direct organogenesis from internodal segments of in vitro grown shoots of apple cv. Golden Delicious Plant Cell Rep. 9:471–474; 1991.
Chaturvedi, H. C.; Sharma, A. K.; Agha, B. Q.; Jain, M.; Sharma, M. Production of cloned trees of Populus deltoides through in vitro regeneration of shoots from leaf, stem and root explants and their field cultivation. Ind. J. Biotechnol. 2:203–208; 2004.
Cheema, G. S. Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature Himalayan poplar (Populus ciliata). Plant Cell Rep. 8:124–127; 1989.
Chen, D.; Huang, M. Culture of the apical tissue of Populus nigra cv. Blanc de Garonne and the variation of their isozymes. J. Nanjing Tech. Coll. For. Prod. 3:104–107; 1995.
Cochran, W. G.; Cox, G. M. Experimental designs, 3rd edn. Bombay: Asia Publishing House; 1963.
Dai, W. H.; Cheng, Z. M.; Sargent, W. Plant regeneration and Agrobacterium-mediated transformation of two elite aspen hybrid clones from in vitro leaf tissues. In vitro Cell. Dev. Biol. Plant. 39:6–11; 2003.
Han, K. H.; Gordon, M. P.; Strauss, S. H.; Han, K. H. High frequency transformation of cotton woods (genus Populus) by Agrobacterium tumefaciens. Can. J. For. Res. 24:464–470; 1997.
Howe, G. T.; Goldfarb, B.; Strauss, S. H. Agrobacterium-mediated transformation of hybrid poplar suspension cultures and regeneration of transformed plants. Plant Cell Tiss. Organ Cult. 36:59–71; 1994.
Ide, Y.; Kurita, N.; Kang, J. M. In vitro plant regeneration from petiole culture of poplar hybrids and their competence for adventitious bud formation. Bull. Tokyo Univ. For. 91:125–135; 1994.
Jafari, M. A.; Kiss, J.; Gergacz, J.; Heszky, L. E. High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes. Acta Biol. Hung. 46:51–59; 1995.
Mehra, P. N.; Cheema, G. S. Clonal multiplication in vitro of Himalayan poplar (Populus ciliata). Phytomorphology 30:336–343; 1980.
Modgil, M.; Handa, R.; Sharma, D. R. Direct shoot regeneration from excised leaves of in vitro raised shoots of clonal apple rootstock, MM106. Curr. Sci. 76:277–278; 1999.
Mohri, T.; Igasaki, T.; Futamura, N.; Shinohara, K. Morphological changes in transgenic poplar induced by expression of the rice homebox gene OSHI. Platn Cell Rep. 18:816–819; 1999.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Planta 15:211–218; 1962.
Shen, H. L.; Wantanabe, S.; Ide, Y. Establishment of a callus culture system of Populus euphratica, Populus alba cv. Pyramidalis, and Populus maximowiczii × Populus plantierensis. Bull. Tokyo Univ. For. 99:19–26; 1998.
Son, S. H.; Hall, R. B. Multiple shoot regeneration from root organ cultures of Populus alba × P. grandidentata. Plant Cell Tiss. Organ Cult. 20:53–57; 1990.
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Thakur, A.K., Srivastava, D.K. High-efficiency plant regeneration from leaf explants of male himalayan poplar (Populus ciliata wall.). In Vitro Cell.Dev.Biol.-Plant 42, 144–147 (2006). https://doi.org/10.1079/IVP2005743
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DOI: https://doi.org/10.1079/IVP2005743
Key words
- acclimatization
- direct organogenesis
- leaf explant
- Populus ciliata
- shoot regeneration