Skip to main content

Advertisement

Springer Nature Link
Log in

RAPD analysis of a variant of banana (Musa sp.) cv. grande naine and its propagation via shoot tip culture

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-derived cultures of the cv. Grande Naine grown on Murashige and Skoog (MS) medium enriched with 13.3 μM N 6-benzyladenine (BA) developed numerous morphological variants after transfer to MS medium with 6.66 μM BA. The variant designated as CUDBT-B1, with distinct morphological features, was further evaluated. The morphological features of CUDBT-B1 were variegated leaf, pseudostem, bracts, ovary of the male flower and fruits, reduced height, decreased lamina length and breadth, and early flowering. These features were also manifested in the second-cycle progeny of CUDBT-B1. RAPD assay showed a marker DNA band of 1650 bp, and differential band intensity between the CUDBT-B1 and normal clone. CUDBT-B1 was multiplied using shoot tip culture, and the shoots were rooted on half-strength MS medium supplemented with 2.69 μM α-naphthaleneacetic acid. All plantlets showed variegated leaves under field conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Damasco, O. P.; Graham, G. C.; Henry, R. J.; Adkins, S. W.; Smith, M. K.; Godwin, I. D. Random amplified polymorphic DNA (RAPD) detection of dwarf off-types in micropropagated Cavendish (Musa spp. AAA) bananas. Plant Cell Rep. 16:118–123; 1996.

    CAS  Google Scholar 

  • Dellaporta, S. L.; Wood, J.; Hicks, J. B. A plant DNA minipreparation. Version II. Plant Mol. Biol. Rep. 1:19–21; 1983.

    CAS  Google Scholar 

  • Donini, P.; Sonnino, A. Induced mutation in plant breeding: current status and future outlook. In: Jain, S. M. ed. Somaclonal variation and induced mutation in crop improvement. Dordrecht: Kluwer Academic Publisher; 1998:225–291.

    Google Scholar 

  • Garcia, L. R.; Perez, P. J.; Bermudez, I. C.; Orellana, P. P.; Veitia, N. R.; Padron, Y. M.; Remero, C. Q. Comparative study of variability produced by induced mutation and tissue culture in banana (Musa sp.) cv. ‘Grande Naine’. INFOMUSA 11:4–6; 2002.

    Google Scholar 

  • Gimenez, C.; De Garcia, E.; De Enrech, N. X.; Blanca, I. Somaclonal variation in banana: cytogenetic and molecular characterization of the somaclonal variant CIEN BTA-03. In Vitro Cell. Dev. Biol. Plant 37:217–222; 2001.

    Article  CAS  Google Scholar 

  • Gubbuk, H.; Pekmezci, M.; Onus, A. N.; Erkan, M. Identification and selection of superior banana phenotypes in the cultivar Dwarf Cavendish using agronomic characteristics and RAPD markers. Pak. J. Bot. 36:331–342; 2004.

    Google Scholar 

  • Hu, J.; Quiros, C. F. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10:505–511; 1991.

    Article  Google Scholar 

  • Hwang, S. C. Somaclonal variational approach to breeding Cavendish banana for resistance to Fusarium wilt race 4. In: Global conference on banana and plantain, Bangalore, India; 2002:57.

  • Hwang, S. C.; Ko, W. H. Cavendish banana cultivars resistant to fusarium wilt acquired through somaclonal variation in Taiwan. Plant Dis. 88:580–588; 2004.

    Google Scholar 

  • Hwang, S. C.; Tang, C. Y. Improved resistance to Fusarium wilt through somaclonal variation in Cavendish (AAA dessert) bananas In: Proceedings of conference on challenges for banana production and utilization in 21st century. Trichy, India: Association for Improvement in Production and Utilization of Banana; 2000:195–208.

    Google Scholar 

  • Israeli, Y.; Reuveni, O.; Lahav, E. Qualitative aspects of somaclonal variations in banana propagated by in vitro techniques. Sci. Hort. 48:71–88; 1991.

    Article  Google Scholar 

  • Larkin, P. J.; Scowcroft, W. Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197–214; 1981.

    Article  Google Scholar 

  • Martin, M. J. G.; Grillo, G. S.; Dominguez, A. M. The use of randomly amplified polymorphic DNA (RAPD) for the study of genetic diversity and somaclonal variation in Musa. Int. Soc. Hort. Sci. 490:445–454; 1998.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays for tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Newbury, H. J.; Howell, E. C.; Crouch, J. H.; Ford-Lloyd, B. V. Natural and cultural induced variation in plantains (Musa spp. AAB group). Aust. J. Bot. 48:493–500; 2000.

    Article  CAS  Google Scholar 

  • Novak, F. J.; Afza, R.; van Duren, M.; Perea-Dallos, M.; Conger, B. V.; Xiaolang, T. Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) cooking (ABB) bananas (Musa spp.). Bio/Technology 7:147–158; 1989.

    Article  Google Scholar 

  • Pérez, J. P. Variación somaclonal. In: Pérez, P. J., ed. Propagación y mejora genética de plantas por biotechnología. Exitoria GEO; 1998:105–221.

  • Prakash, S.; Elangomathavan, R.; Seshadri, S.; Kathiravan, K.; Ignacimuthu, S. Efficient regeneration of Curcuma amada Roxb. plantlets from rhizome and leaf sheath explants. Plant Cell Tiss. Organ Cult. 78:159–165; 2004.

    Article  CAS  Google Scholar 

  • Ramage, C. M.; Borda, A. M.; Hamill, S. D.; Smith, M. K. A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA). Sci. Hort. 103:145–151; 2004.

    Article  CAS  Google Scholar 

  • Sahijram, L.; Soneji, J. R.; Bollamma, K. T. Analysing somaclonal variation in micropropagated bananas (Musa spp.). In Vitro Cell. Dev. Biol. Plant 39:551–556; 2003.

    Article  Google Scholar 

  • Shepherd, K.; Souza, F. V. D.; Da Silva, K. M. Mitotic instability in banana varieties IV. BAP concentration and effects of number of subcultures. Fruits 51:211–216; 1996.

    Google Scholar 

  • Shu, Q. Y.; Liu, G. S.; Qi, D. M.; Chu, C. C.; Liu, J.; Li, H. An effective axillary bud culture and RAPD analysis of clone plants in tetraploid black locust. Plant Cell Rep. 22:175–180; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Singh, H. P. Indian bananas—issues and strategies In: Global conference on banana and plantain. Bangalore, India; 2002:1–2.

  • Stover, R. H. Somaclonal variation in Grande Naine and Saba banana in nursery and field. ACIAR Proc. series, Australian Center for International Agricultural Research 21:136–139; 1987.

  • Tang, C. Y.; Liu, C. C.; Hwang, S. Improvement of the horticultural traits of Cavendish banana (Musa spp. AAA group I). Selection and evaluation of semi-dwarf clone resistance Fusarium wilt. J. Chin. Soc. Hort. Sci. 46:173–182; 2000.

    Google Scholar 

  • Tingey, S. V.; del Tufo, J. P. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol. 101:349–352; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Trujillo, I.; Garcia, E. Strategies for obtaining somaclonal variants resistant to Yellow Sigatoka (Mycosphaerella musicola). INFOMUSA 5:12–13; 1996.

    Google Scholar 

  • Vidal, M. D. C.; de Garcia E. Analysis of a Musa spp. somaclonal variant resistant to Yellow Sigatoka. Plant Mol. Biol. Rep. 18:23–31; 2000.

    Google Scholar 

  • Vidhya, R.; Ashalatha, S. N. In vitro culture, pseudostem pigmentation and genetic characterization of Musa acuminata cv. Red. In: Global conference on banana and plantain. Bangalore, India; 2002:65.

  • Williams, J. G. K.; Hanafey, M. K.; Rafalski, J. A.; Tingey, S. V. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol. 218:704–740; 1993.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Calicut, 673 635, Kerala, India

    K. P. Martin, Suneesh Kumar Pachathundikandi & Joseph Madassery

  2. Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, The Gateway, LE1 9BH, Leicester, UK

    C. -L. Zhang & A. Slater

Authors
  1. K. P. Martin
    View author publications

    Search author on:PubMed Google Scholar

  2. Suneesh Kumar Pachathundikandi
    View author publications

    Search author on:PubMed Google Scholar

  3. C. -L. Zhang
    View author publications

    Search author on:PubMed Google Scholar

  4. A. Slater
    View author publications

    Search author on:PubMed Google Scholar

  5. Joseph Madassery
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Joseph Madassery.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martin, K.P., Pachathundikandi, S.K., Zhang, C.L. et al. RAPD analysis of a variant of banana (Musa sp.) cv. grande naine and its propagation via shoot tip culture. In Vitro Cell.Dev.Biol.-Plant 42, 188–192 (2006). https://doi.org/10.1079/IVP2005736

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1079/IVP2005736

Key words