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In vitro culture response of common bean explants to filtrate from Pseudomonas syringae pv. Phaseolicola and correlation with disease resistance

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Summary

The in vitro culture responses from different explants of a race-specific resistant cultivar (Red Mexican) and a racesusceptible cultivar (Palmeña) to halo-blight pathogen (Pseudomonas syringae pv. phaseolicola were studied. Two kinds of filtrate obtained from a phaseolotoxin producer wild type and a non-producer mutant of P. syringae pv. phaseolicola race-7 were used. Callus formation of Red Mexican was significantly reduced in the presence of phaseolotoxin. Bud-shoot growth was more sensitive than callus formation to other metabolites present in the pathogen filtrate, but the presence of phaseolotoxin in the media showed a positive correlation between resistance to halo blight race-7 pathogen and bud-shoot growth. Our results indicate that differential in vitro responses are influenced by the plant genotype and by the metabolite composition and concentration of the filtrate.

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References

  • Arai, M.; Takeuchi, M. Influence of Fusarium wilt toxin(s) on Carnation cells. Plant Cell Tiss. Organ Cult. 34:287–293; 1993.

    Article  CAS  Google Scholar 

  • Bachmann, A. S.; Matile, P.; Slusarenko, A. J Inhibition of ornithine decarboxylase activity by phaseolotoxin: implications for symptom production in halo blight of French bean. Physiol. Mol. Plant Pathol. 53:287–299; 1998.

    Article  CAS  Google Scholar 

  • Bajaj, Y. P. S.; Saettler, A. W. Effect of halo toxin-containing filtrates of Pseudomonas phaseolicola on the growth of bean callus tissue. Phytopathology 60:1065–1067; 1970.

    Article  CAS  Google Scholar 

  • Bender, C. L. Chlorosis-inducing phytotoxins produced by Pseudomonas syringae. Eur. J. Plant Pathol. 105:1–12; 1999.

    Article  CAS  Google Scholar 

  • Bender, C. L.; Alarcón-Chaidez, F.; Gross, D. C. Pseudomonas syringae phytotoxins: mode of action, regulation, and biosynthesis by peptide and polyketide synthetases. Microbiol. Mol. Biol. Rev. 63:266–292; 1999.

    PubMed  CAS  Google Scholar 

  • Fernández, M. T.; Fernández, M. L.; Centeno, M. L.; Cañal, M. J.; Rodriguez, R. Reaction of common bean callus to culture filtrate of Colletotrichum lindemuthianum: differences in the composition and toxic activity of fungal culture filtrates. Plant Cell Tiss. Organ Cult. 61:41–49; 2000.

    Article  Google Scholar 

  • Franklin C. I.; Trieu, T. N.; Gonzales, R. A.; Dixon, R. A Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L.) via organogenesis. Plant Cell Tiss. Organ Cult. 24:199–206; 1991.

    Article  Google Scholar 

  • González, A. I.; Pérez de la Vega, M.; Ruiz, M. L.; Polanco, C. Análisis of the argK-tox gene cluster in nontoxigenic strains of Pseudomonas syringae pv. Phaseolicola. Appl. Environ. Microbiol. 69:4979–4982; 2003.

    Article  PubMed  CAS  Google Scholar 

  • González, A. I.; Polanco, C.; Ruiz, M. L. Protein pattern changes induced by culture filtrate of Pseudomonas syringae pv. phaseolicola in bean (Phaseolus vulgaris L.) explants cultured in vitro. Agronomie 20:673–682; 2000.

    Article  Google Scholar 

  • Goss, R. W The relation of temperature to common and halo blight of beans. Phytopathology 30:258–264; 1940.

    Google Scholar 

  • Hartman, C. L.; McCoy, T. J.; Knous, T. R. Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporum f. sp. medicaginis Plant Sci. Lett. 34:183–194; 1984.

    Article  Google Scholar 

  • Hartman, C. L.; Secor, G. A.; Venette, J. R.; Albaugh, D. A Response of bean calli to filtrate from Pseudomonas syringae pv. phaseolicola and correlation with whole plant disease reaction. Physiol. Mol. Plant Pathol. 28:353–358; 1986.

    Google Scholar 

  • Jayasankar, S.; Li, Z.; Gray, D. J. In-vitro selection of Vitis vinifera ‘Chardonnay’ with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase. Planta 211:200–208; 2000.

    Article  PubMed  CAS  Google Scholar 

  • Jenner, C.; Hitchin, E.; Mansfield, J.; Walters, K.; Betteridge, P.; Teverson, D.; Taylor, J. Gene-for-gene interactions between Pseudomonas syringae pv. phaseolicola and Phaseolus. Mol. Plant-Microbe Interact. 4:553–562; 1991.

    PubMed  CAS  Google Scholar 

  • Jensen, J. H.; Livingstone, J. E. Variation in symptoms produced by isolates of Phytomonas medicaginis var. phaseolicola. Phytopathology 34:471–480; 1944.

    Google Scholar 

  • Jin, H.; Hartman C. L.; Huang, Y. H.; Nickell, C. D.; Widholm, J. M. Regeneration of soybean plants from embryogenic suspension cultures treated with toxic culture filtrate of Fusarium solani and screening of regenerants for resistance. Phytopathology 86:714–718; 1996a.

    Article  Google Scholar 

  • Jin, H.; Hartman, C. L.; Nickell, C. D.; Widholm, J. M Phytotoxicity of culture filtrate from Fusarium solani, the causal agent of sudden death syndrome of soybean. Plant Dis. 80:922–927; 1996b.

    Article  Google Scholar 

  • King, E. O.; Ward, M. K.; Raney, D. E Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301–307; 1954.

    PubMed  CAS  Google Scholar 

  • Maniatis, T.; Fritsh, E. F.; Sambrook, J. Molecular cloning: a laboratory manual. Cold Sping Harbor, NY: Cold Spring Harbor Laboratory Press; 1982.

    Google Scholar 

  • Mansfield, J.; Jenner, C.; Hockenhull, R.; Bennett, M. A.; Stewart, R. Characterization of avrPphE, a gene for cultivar-specific avirulence from Pseudomonas syringae pv. phaseolicola which is physically linked to hrpY, a new hrp gene identified in the halo-blight bacterium. Mol. Plant-Microbe Interact. 7:725–739; 1994.

    Google Scholar 

  • McClean, P.; Grafton, K. F Regeneration of dry bean (Phaseolus vulgaris L.) via organogenesis. Plant Sci. 60:117–122; 1989.

    Article  Google Scholar 

  • Mitchell, R. E. Bean halo-blight toxin. Nature 260:75–76; 1976.

    Article  CAS  Google Scholar 

  • Moore, R. E.; Niemczura, W. P.; Kwork, O. C. H.; Patil, S. S. Inhibitors of ornithine carbamoyltransferase from Pseudomonas syringae pv. phaseolicola. Revised structure of phaseolotoxin Tetrahedron Lett. 25:3931–3934; 1984.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Nüske, J.; Fritsche, W. Phaseolotoxin production by Pseudomonas syringae pv. phaseolicola: the influence of temperature. J. Basic Microbiol. 29:441–447; 1989.

    Article  PubMed  Google Scholar 

  • Patel, P. N.; Walker, J. C. Relation of air temperature and age and nutrition of the host to the development of halo and common bacterial blights of bean. Phytopathology 53:407–411; 1962.

    Google Scholar 

  • Patil, S. S.; Hayward, A. C.; Emmons, R. An ultraviolet-induced nontoxigenic mutant of Pseudomonas phaseolicola of altered pathogenicity. Phytopathology 64:590–595; 1974.

    Article  Google Scholar 

  • Peet, R. C.; Lindgren, P. B.; Willis, D. K.; Panopoulos, N. J Identification and cloning of genes involved in phaseolotoxin production by Pseudomonas syringae pv. phaseolicola. J. Bacteriol. 166:1096–1105; 1986.

    PubMed  CAS  Google Scholar 

  • Ruiz, M. L.; Pelaez, M. I.; Rueda, J.; Espino, F. J.; Vazquez, A. M. A comparative study of callus formation and plant regeneration from different explants of Phaseolus vulgaris and P. coccineus. In: Horn, J.; Odenbach, S., eds. Genetic manipulation in plant breeding. New York: Walter de Gruyter Co.; 1986:495–497.

    Google Scholar 

  • Russell, P. E. Variation in the virulence of some streptomycin resistant mutants of Pseudomonas phaseolicola. J. Appl. Bacteriol. 39:175–180; 1975.

    PubMed  CAS  Google Scholar 

  • Staskawicz, B. J.; Panopoulos, N. J. A rapid and sensitive microbiological assay for phaseolotoxin. Phytopathology 69:663–666; 1979.

    Article  CAS  Google Scholar 

  • Thanutong, P.; Furusawa, I.; Yamamoto, M. Resistant tobacco plants from protoplast-derived calluses selected for their resistance to Pseudomonas and Alternaria toxins. Theor. Appl. Genet. 66:209–215; 1983.

    Google Scholar 

  • Tsiamis, G.; Mansfield, J.W.; Hockenhull, R.; Jackson, R. W.; Sesma, A.; Athanassopoulos, E.; Bennett, M. A.; Stevens, C.; Vivian, A.; Taylor J. D.; Murillo, J. Cultivar-specific avirulence and virulence functions assigned to avrPphF in Pseudomonas syringae pv. phaseolicola, the cause of bean halo-blight disease. EMBO J. 19:3204–3214; 2000.

    Article  PubMed  CAS  Google Scholar 

  • Vaquero, F.; Robles, C.; Ruiz, M. L. A method for long-term micropropagation of Phaseolus coccineus L. Plant Cell Rep. 12:395–398; 1993.

    Article  CAS  Google Scholar 

  • Venkatachalam, P.; Rao, K. S.; Kishor, P. B.; Jayabalan, N. Regeneration of late leaf spot-resistant groundnut plants from Cercosporidium personatum culture filtrate-treated callus. Curr. Sci. 74:61–65; 1998.

    Google Scholar 

  • Vivian, A.; Gibbon, M. J.; Murillo, J. The molecular genetics of specificity determinants in plant pathogenic bacteria. In Crute, I. R.; Holub, E. B.; Burdon, J. J., eds. The gene for gene relationship in plant-parasite interactions. New York: CAB International: 1997:293–328.

    Google Scholar 

  • Yoder, O. C. Toxins in pathogenesis. Ann. Rev. Phytopathol. 18:103–129; 1980.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Área de Genética, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, E-24071, León, Spain

    A. I. González, C. Polanco & M. L. Ruiz

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  1. A. I. González
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  2. C. Polanco
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  3. M. L. Ruiz
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Correspondence to M. L. Ruiz.

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González, A.I., Polanco, C. & Ruiz, M.L. In vitro culture response of common bean explants to filtrate from Pseudomonas syringae pv. Phaseolicola and correlation with disease resistance. In Vitro Cell.Dev.Biol.-Plant 42, 160–164 (2006). https://doi.org/10.1079/IVP2005729

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  • DOI: https://doi.org/10.1079/IVP2005729

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