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Simple one-medium formulation regeneration of fingerroot [Boesenbergia rotunda (L.) mansf. Kulturpfl.] via somatic embryogenesis

In Vitro Cellular & Developmental Biology - Plant volume 41, pages 757–761 (2005)Cite this article

Summary

Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54μM 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 ±4.3% on this MS medium augmented by 13.54μM 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6±0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil.

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Authors and Affiliations

  1. Sunway University College, 5 Jalan Kolej, Bandar Sunway, PJ 46150, Selangor, Malaysia

    S. K. Tan

  2. Western Michigan University, 1903 West Michigan Ave, 49008-5211, Kalamazoo, MI

    R. Pippen

  3. Biochemistry Department, Medical Faculty, University Malaya, 50603, Kuala Lumpur, Malaysia

    R. Yusof

  4. Institute of Biological Sciences, Science Faculty, University Malaya, 50603, Kuala Lumpur, Malaysia

    H. Ibrahim & N. Khalid

  5. Chemistry Department, Science Faculty, University Malaya, 50603, Kuala Lumpur, Malaysia

    N. Rahman

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  1. S. K. Tan
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  2. R. Pippen
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  3. R. Yusof
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  4. H. Ibrahim
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  5. N. Rahman
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  6. N. Khalid
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Correspondence to S. K. Tan.

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Tan, S.K., Pippen, R., Yusof, R. et al. Simple one-medium formulation regeneration of fingerroot [Boesenbergia rotunda (L.) mansf. Kulturpfl.] via somatic embryogenesis. In Vitro Cell.Dev.Biol.-Plant 41, 757–761 (2005). https://doi.org/10.1079/IVP2005695

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  • DOI: https://doi.org/10.1079/IVP2005695

Key words

  • auxins
  • micropropagation
  • ginger
  • Zingiberaceae