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Effects of genotype, explant orientation, and wounding on shoot regeneration in tomato

In Vitro Cellular & Developmental Biology - Plant volume 41, pages 457–464 (2005)Cite this article

Summary

Effects of genotype and explant orientation on shoot regeneration from cotyledonary explants of tomato were studied using 10 commercially important cultivars. The explant orientation affected shoot regeneration in all the tested genotypes. Cotyledons placed in abaxial (lower surface facing down) orientation consistently produced better shoot regenerative response and produced greater numbers and taller shoots compared to those inoculated in adaxial (upper surface facing down) orientation. Genotypic variation in terms of shoot regeneration response, number of shoots, and shoot height was apparent. Wounding of cotyledonary explants increased shoot regeneration response. However, shoot height was much lower in shoots regenerated from wounded explants compared to those that originated from intact cotyledons. Shoots produced from wounded cotyledons were abnormal in their form and structure.

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Author notes

  1. Poonam Bhatia

    Present address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-0032, Tokyo, Japan

Authors and Affiliations

  1. Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, 4702, Rockhampton, QLD, Australia

    Poonam Bhatia, Nanjappa Ashwath & David J. Midmore

Authors
  1. Poonam Bhatia
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  2. Nanjappa Ashwath
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  3. David J. Midmore
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Correspondence to Poonam Bhatia.

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Bhatia, P., Ashwath, N. & Midmore, D.J. Effects of genotype, explant orientation, and wounding on shoot regeneration in tomato. In Vitro Cell.Dev.Biol.-Plant 41, 457–464 (2005). https://doi.org/10.1079/IVP2005649

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  • DOI: https://doi.org/10.1079/IVP2005649

Key words

  • Lycopersicon esculentum
  • micropropagation
  • morphogenesis