Direct plant regeneration of curry leaf tree (Murraya koenigii koenig.), an aromatic plant


An efficient protocol for plant regeneration from stem segments of Murraya koenigii was developed by culturing on Murashige and Skoog (MS) medium supplemented with 2.5 mg l−1 benzyladenine (BA), 25 mgl−1 adenine sulfate, 0.25 mgl−1 indole-3-acetic acid (IAA), and 3% sucrose. The frequency of shoot bud regeneration was higher on similar medium in subsequent subcultures. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.25–0.5 mgl−1 IAA or 1-naphthaleneacetic acid (NAA) within 8–12 d of culture. The maximum percentage of rooting was obtained on MS medium supplemented with IAA and NAA, each at 0.25 mgl−1. During acclimatization, 95% of rooted plantlets survived were grown normally under greenhouse conditions.

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Correspondence to Gyana Ranjan Rout.

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Rout, G.R. Direct plant regeneration of curry leaf tree (Murraya koenigii koenig.), an aromatic plant. In Vitro Cell.Dev.Biol.-Plant 41, 133–136 (2005).

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Key words

  • genetic fidelity
  • in vitro
  • medicinal plant
  • plant regeneration
  • RAPD marker
  • stem segments