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Direct plant regeneration of curry leaf tree (Murraya koenigii koenig.), an aromatic plant

  • Gyana Ranjan RoutEmail author
Article

Summary

An efficient protocol for plant regeneration from stem segments of Murraya koenigii was developed by culturing on Murashige and Skoog (MS) medium supplemented with 2.5 mg l−1 benzyladenine (BA), 25 mgl−1 adenine sulfate, 0.25 mgl−1 indole-3-acetic acid (IAA), and 3% sucrose. The frequency of shoot bud regeneration was higher on similar medium in subsequent subcultures. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.25–0.5 mgl−1 IAA or 1-naphthaleneacetic acid (NAA) within 8–12 d of culture. The maximum percentage of rooting was obtained on MS medium supplemented with IAA and NAA, each at 0.25 mgl−1. During acclimatization, 95% of rooted plantlets survived were grown normally under greenhouse conditions.

Key words

genetic fidelity in vitro medicinal plant plant regeneration RAPD marker stem segments 

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Copyright information

© Society for In Vitro Biology 2005

Authors and Affiliations

  1. 1.Plant Tissue Culture Laboratory, Plant Biotechnology DivisionRegional Plant Resource CentreBhubaneswarIndia

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