Summary
Tylophora indica (Burm. f.) Merrill is a threatened medicinal climber distributed in the forests of northern and peninsular India. An efficient and reproducible protocol for high-frequency callus regeneration from immature leaf explants of T. indica was developed. Organogenic callus formation from immature leaf pieces was obtained by using Murashige and Skoog (MS) medium supplemented with 7 μM 2,4-dichlorophenoxyacetic acid and 1.5 μM 6-benzyladenine. On this medium 92% explants produced callus. The optimal hormone combination for plantlet regeneration was 8 μM thidiazuron, at which shoot regeneration was obtained from 100% of the cultures, with an average of 66.7 shoots per culture. Histological studies of the regenerative callus revealed that shoot buds were originated from the outermost regions. For root formation, half-strength MS medium supplemented with 3 μM indole-3-butyric acid was used. Plants were transferred to soil, where 92% survived after 3 mo. of acclimatization.
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Thomas, T.D., Philip, B. Thidiazuron-induced high-frequency shoot organogenesis from leaf-derived callus of ia medicinal climber, Tylophora Indica (Burm. F.) merrill. In Vitro Cell.Dev.Biol.-Plant 41, 124–128 (2005). https://doi.org/10.1079/IVP2004575
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DOI: https://doi.org/10.1079/IVP2004575