In Vitro Cellular & Developmental Biology - Plant

, Volume 40, Issue 5, pp 464–466 | Cite as

In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree Pterocarpus marsupium Roxb.

  • Suresh ChandEmail author
  • Ajay Kumar Singh


A protocol has been developed for in vitro plant regeneration from cotyledonary nodes of Pterocarpus marsupium Roxb. Multiple shoots were induced from cotyledonary nodes derived from 20-d-old axenic seedlings grown on Murashige and Skoog (MS) medium containing 2.22–13.32 μM benzyladenine (BA) or 2.32–13.93 μM kinetin alone or in combination with 0.26 μM α-naphthaleneacetic acid (NAA). The highest frequency of responding explants (85%) and maximum number of shoots per explant (9.5) were obtained on MS medium supplemented with 4.44 μM BA and 0.26 μM NAA after 15 wk of culture. A proliferating shoot culture was established by repeatedly subculturing the orginal cotyledonary nodal explant on fresh medium after each harvest of the newly formed shoots. Nearly 30% of the shoots formed roots after being transferred to half-strength MS medium containing 9.84 μM indole-3-butyric acid after 25 d of culture. Fifty percent of shoots were also directly rooted as microcuttings on peat moss, soil, and compost mixture (1∶1∶1). About 52% plantlets rooted under ex vitro conditions were successfully acclimatized and established in pots.

Key words

ex vitro rooting Malabar Kino Pterocarpus marsupium plant regeneration shoot multiplication 


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Copyright information

© Society for In Vitro Biology 2004

Authors and Affiliations

  1. 1.Plant Tissue Culture and Genetics Research Group, School of Life SciencesDevi Ahilya UniversityIndoreIndia

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