Skip to main content
SpringerLink
Log in

In vitro propagation of Pluchea lanceolata, a medicinal plant, and effect of heavy metals and different aminopurines on quercetin content

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

An effcient regeneration protocol has been developed from leaf explants of Pluchea lanceolata, a medicinally important plant of the family Asteracea. Nodular callus was initiated from young leaf segments cultured on Wood and Braun medium containing 2.0% sucrose (WB) supplemented with kinetin. On WB+5.0 mg l−1 kinetin, 100% plant regeneration with 14±0.5 plantlets was obtained. Regenerated plantlets with well-developed root systems were transferred to pots and subsequently to the field. With respect to the effect of metals on morphogenic events and quercetin content of the cultures, lower concentrations of CuSO4 proved to be beneficial but higher concentrations were detrimental. The presence of 200 μM ZnSO4 or 150 μM CuSO4 also resulted in the highest level of quercetin in regenerants. The yield of quercetin in culture varied with stages of differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ali, G.; Purohit, M.; Saba; Iqbal, M.; Srivastava, P. S. Morphogenic response and isozymes of Bacopa monniera L. Wettst cultures grown under salt stress. Phytomorphology 47:97–106; 1997.

    Google Scholar 

  • Ali, G.; Srivastava, P. S.; Iqhal, M. Morphogenic response and proline content in Bacopa monniera cultures grown under copper stress. Plant Sci. 138:191–195; 1998.

    Article  CAS  Google Scholar 

  • Anonymous. The wealth of India. Raw materials, vol. VIII. New Delhi: Council of Scientific and Industrial Research; 1998.

  • Asami, T.; Kuboto, M.; Orikasa, K. Distribution of different fractions of cadmium, zinc, lead and copper in unpolluted and polluted soil. Soil Water Air Pollut. 83:187–194; 1995.

    Article  CAS  Google Scholar 

  • Bates, L. K.; Waldren, R. P.; Teare, J. D. Rapid determination of free proline for water stress studies. Plant Soil 39:205–207; 1973.

    Article  CAS  Google Scholar 

  • Bhattacharya, R.; Bhattacharya, S. High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture. Ind. J. Exp. Biol. 39:1184–1187; 2001.

    Google Scholar 

  • Borthakur, M.; Dutta, K.; Nath, S. C.; Singh, R. S. Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique. Plant Cell Tiss. Organ Cult. 62:239–242; 2000.

    Article  Google Scholar 

  • Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254; 1976.

    Article  PubMed  CAS  Google Scholar 

  • Chen, C. T.; Kao, C. H. Osmotic stress and water stress, have opposite effects on putrescine and proline production in excised rice leaves. Plant Growth Regul 13:197–202; 1993.

    Article  Google Scholar 

  • Datta, A.; Srivastava, P. S. Variation in vinblastin production by Catharanthus roscus during in vivo and in vitro differentiation. Phytochemistry 46:135–137; 1997.

    Article  CAS  Google Scholar 

  • Davies, K. L.; Davies, M. S.; Francis, D. Zinc induced vacuolation in root meristematic cells of Festuca rubra L. Plant Cell Environ. 14:399–406; 1991.

    Article  CAS  Google Scholar 

  • Delauney, A. J.; Verma, D. P. S. Proline biosynthesis and osmoregulation in plants. Plant J. 4:215–223; 1993.

    Article  CAS  Google Scholar 

  • Fernandes, J. C.; Henriques, F. S. Biochemical, physiological and structural effects of excess copper in plants. Bot. Rev. 57:246–276; 1991.

    Google Scholar 

  • Garcia-Sago, B.; Roig, L. A.; Moreno, V. Enhancement of morphogenetic response in cotyledon-derived explants of Cucumis melo induced by copper ions. Acta Hort. 289:229–230; 1991.

    Google Scholar 

  • Godbold, D. L.; Horst, W. J.; Marschner, H.; Collins, J. C.; Thurman, D. A. Root growth and Zn uptake by two ecotypes of Deschampsia caespitosa as affected by high Zn concentrations. Z. Pflanzenphysiol. 112:315–324; 1983.

    CAS  Google Scholar 

  • Grieve, T. M.; Gartland, K. M. A.; Elliott, M. C. Micropropagation of commercially important sugarbeet cultivars. Plant Growth Regul. 21:15–18; 1997.

    Article  CAS  Google Scholar 

  • Macnair, M. R. The genetics of metal tolerance in vascular plants. New Phytol. 124:641–559; 1992.

    Google Scholar 

  • Marschner, H. Nutrition of higher plants. New York: Academic Press; 1986.

    Google Scholar 

  • Mengel, K.; Kirby, E. A. Principles of plant nutrition, 4th edn. Bern: International Potash Institute; 1987:385–402.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Ouzounidou, G. Copper induced changes on growth, metal content and photosynthetic function of Alyssum montanum L. plants. Environ. Exp. Bot. 34:165–172; 1994.

    Article  CAS  Google Scholar 

  • Purohit, M.; Pande, D.; Datta, A.; Srivastava, P. S. Enhanced xanthotoxin content in regenerating cultures of Ammi majus and micropropagation. Planta Med. 61:481–482; 1995a.

    Article  PubMed  CAS  Google Scholar 

  • Purohit, M.; Pande, D.; Datta, A.; Srivastava, P. S. In vitro flowering and high xanthotoxin in Ammi majus L. J. Plant Biochem. Biotechnol. 4:73–76; 1995b.

    Google Scholar 

  • Saba; Pande, D.; Iqbal, M.; Srivastava, P. S. Effect of zinc sulphate and copper sulphate on regeneration and lepidine content in Lepidium sativum, L Biol. Plant. 43:1–4; 1999.

    Google Scholar 

  • Saunders, J. W.; Doley, W. P. One step shoot regeneration form callus of whole plant leaf explants of sugarbeet lines and somaclonal variation of in vitro culture behaviour. J. Plant Physiol. 124:473–479; 1986.

    CAS  Google Scholar 

  • Srivastava, P. S.; Pande, D. In vitro propagation and conservation of medicinal plants. In: Srivastava, P. S., ed. Plant tissue culture and molecular biology. New Delhi: Narosa Publishing House; 1998:254–281.

    Google Scholar 

  • Srivastava, P. S.; Purohit, M.; Pande., D.; Dutta, A. Phenotypic variation and alkaloid content in the androgenic plantlets of Datura innoxia. Phytomorphology 13:209–216; 1993.

    Google Scholar 

  • Srivastava, V.; Verma, N.; Tandon, J. S.; Srimal, R. C. Anti-inflammatory activity of Pluchea lanccolata: isolation of an active principle. Int. J. Crude Drug Res. 28:135–137; 1990.

    Google Scholar 

  • Tao, R.; Murayama, H.; Moriguchi, K.; Sugiura, A. Plant regeneration from callus cultures derived from primordial leaves of adult Japanese persimmon. HortScience 23:1055–1056; 1998.

    Google Scholar 

  • Tao, R.; Sugiura, A. Adventitious bud formation from callus cultures of Japanese persimmon. HortScience 27:259–261; 1992.

    Google Scholar 

  • Tetu, T.; Sangwan, R. S.; Sangwan-Norreel, B. S. Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J. Exp. Bot. 38:506–517; 1987.

    Article  CAS  Google Scholar 

  • Tiwati, V.; Tiwari, K. N.; Singh, B. D. Comparative studies of cytokinins on in vitro propagation of Bacopa monnierd. Plant Cell Tiss. Organ Cult. 66:9–16; 2001.

    Article  Google Scholar 

  • Watad, A. A.; Kochba, M.; Nissima., A.; Gaba, V. Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane raft. Plant Cell Rep. 40:345; 1995.

    Google Scholar 

  • Wood, H. N.; Braun, A. C. Studies on the regulation of certain essential biosynthetic systems in normal and crown gall tumour Proc. Natl Acad. Sci. USA 147:1507; 1961.

    Google Scholar 

  • Zhong, Z.; Smith, H. G.; Thomas, T. H. In vitro culture of petioles and intact leaves of sugar beet (Beta vulgaris) Plant Growth Regul. 12:59–66; 1993.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Amity Institute of Biotechnology, Amity IT University Campus, Sector 125, 201 301, Noida, Uttar Pradesh, India

    Sanjeev Kumar

  2. Centre for Biotechnology, Hamdard University, 110 062, New Delhi, India

    Alka Narula & P. S. Srivastava

  3. Department of Botany, Faculty of Science, Hamdard University, 110 062, New Delhi, India

    M. P. Sharma

Authors
  1. Sanjeev Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alka Narula
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. P. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. S. Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. S. Srivastava.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumar, S., Narula, A., Sharma, M.P. et al. In vitro propagation of Pluchea lanceolata, a medicinal plant, and effect of heavy metals and different aminopurines on quercetin content. In Vitro Cell.Dev.Biol.-Plant 40, 171–176 (2004). https://doi.org/10.1079/IVP2003490

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1079/IVP2003490

Key words

Search

Navigation