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Plant regeneration from protocorm-derived callus of Cypripedium formosanum


Totipotent callus of Cypripedium formosanum, an endangered slipper orchid species, was induced from seed-derived protocorm segments on a quarter-strength Murashige and Skoog medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron). This callus proliferated well and was maintained by subculturing on the same medium. On average, 13 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the medium with 4.44 μM N6-benzyladenine after 8 wk of culture. The regenerated protocorm-like bodies formed shoots and roots on medium containing 1 g l−1 activated charcoal and 20 g l−1 potato homogenate. After 24 wk of culture on this medium, well-developed plantlets ready for potting were established.

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Correspondence to Yung-I Lee.

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Lee, YI., Lee, N. Plant regeneration from protocorm-derived callus of Cypripedium formosanum . In Vitro Cell.Dev.Biol.-Plant 39, 475 (2003).

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Key words

  • Cypripedium formosanum
  • callus induction
  • plant regeneration
  • protocorm-like body