In Vitro Cellular & Developmental Biology - Plant

, Volume 39, Issue 5, pp 520–523 | Cite as

In vitro culture studies on Stevia rebaudiana

  • Latha Sivaram
  • Usha MukundanEmail author


Shoot apex, nodal, and leaf explants of Stevia rebaudiana Bertoni can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 8.87 μM) and indole-3-acetic acid (5.71 μM). Rooting of the in vitro-derived shoots could be achieved following subculture onto auxin-containing medium. A survival rate of 70% was recorded at the hardening phase on the substrate cocopeat. The presence of the sweet diterpene glycosides, viz. stevioside and rebaudioside, was confirmed in the in vitro-derived tissues of Stevia using HPTLC techniques. Callus cultured on agar-solidified MS medium supplemented with BA (8.87 μM) and indole-3-butyric acid (9.80 μM) showed the highest sweetener content.

Key words

Stevia rebaudiana stevioside micropropagation tissue culture 


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Copyright information

© Society for In Vitro Biology 2003

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of Biological SciencesRamniranjan Jhunjhunwala CollegeMumbaiIndia

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