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High-frequency multiple shoot regeneration from cotyledonary nodes of guar (Cyamopsis tetragonoloba L. Taub)

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Summary

Guar (Cyamopsis tetragonoloba L. Taub) is a drought-tolerant multipurpose cash crop. A rapid regeneration system has been developed for four Indian guar genotypes. Investigations were carried out to assess the effect of different growth regulators and their combinations on a variety of explants such as the embryo, cotyledons, and cotyledonary nodes for shoot morphogenesis. It was established that Murashige and Skoog's culture medium containing 6-benzylaminopurine (13.3 μM or 3 mgl−1) in combination with indole-3-acetic acid (11.4 μM or 2mgl−1) with cotyledonary node explants gave the highest frequency of multiple shoot induction. In vitro rooting from cultured shoots was maximal on a half-salt concentration of Murashige and Skoog's culture medium fortified with indole-3-butyric acid (4.9 μM or 1 mgl−1). In vitro-regenerated plants were grown to pod setting and subsequent maturity in greenhouse conditions.

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Authors and Affiliations

  1. Bioresources and Biotechnology Division, Tata Energy Research Institute School of Advance Studies, Darbari Seth Block, India Habitat Center, Lodhi Road, 110003, New Delhi, India

    Deepak Prem

  2. Department of Genetics, College of Basic Sciences and Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 123005, Haryana, India

    Subhadra Singh

  3. Department of Plant Breeding, Forage Section, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 123005, Haryana, India

    Padam Prakash Gupta & Jaivir Singh

  4. Department of Plant Breeding, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 123005, Haryana, India

    Gaurav Yadav

Authors
  1. Deepak Prem
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  2. Subhadra Singh
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  3. Padam Prakash Gupta
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  4. Jaivir Singh
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  5. Gaurav Yadav
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Correspondence to Deepak Prem.

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Prem, D., Singh, S., Gupta, P.P. et al. High-frequency multiple shoot regeneration from cotyledonary nodes of guar (Cyamopsis tetragonoloba L. Taub). In Vitro Cell.Dev.Biol.-Plant 39, 384–387 (2003). https://doi.org/10.1079/IVP2003425

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  • DOI: https://doi.org/10.1079/IVP2003425

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