Summary
The qualitative and quantitative composition of secondary metabolites was studied in the shoots and roots of Centaurium pulchellum cultured in vitro. Secoiridoids (gentiopierin, swertiamarin, and sweroside) and xanthones (methylbellidifolin, demethyleustomin, and deccussatin) were isolated. Sweroside was found to be the major secoiridoid compound in the aerial parts of plants growing in nature. while swertiamarin dominated in plants cultured in vitro. In roots of all plants, genciopicrin was the major compound. Xanthone demethyleustomin was the major compound both in the shoots and roots of plants growing in nature and cultured in vitro. Different sugars (glucose, fructose, and sucrose) added in different concentrations in the medium affected the production of secondary compounds.
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Ghosal, S.; Sharma, P. V.; Chaudhuri, R. K.; Bhattacharya, S. K. Chemical constituents of Gentianaceae XIV: tetraoxygenated and oxygenated xanthones of Swertia purpurascens. J. Pharm. Sci. 64:80–86;1975.
Hostettmann, K.; Hostettmann, M. Xanthones. In:Dey, P. M.; Harborn, J. B., eds. Methods in plant biochemistry. New York; Academic Press; 1989:493–535.
Ishimary, K.; Sudo, H.; Satake, M.; Matsunaga, Y.; Hasegawa, Y.; Takemoto, S.; Shimomura, K. Amarogentin, amaroswerin and four xanthones from hairy roots cultures of Swertia japonica. Phytochemistry 29:1563–1565; 1990.
Jankovié, T.; Krstié, D.; Ŝavikin-Fodulovic, K.; Menkovié, N.; Grubiŝié, D. Xanthone compounds of Centaurium erythraea grown in nature and cultured in vitro. Pharm. Pharmacol. Lett. 10:23–25; 2000.
Khafagy, S. M.; Mnajed, H. K. Phytochemical investigation of Centaurium pulchellum (Sw) Druce. Acta Pharm. Suecia 7:667–671; 1970.
Kondo, Y.; Takano, F.; Hojo, H. Suppression of chemically and immunologically induced hepatic injuries by gentiopieroside in mice. Planta Med. 60:414–416; 1994.
Krstić, D. Ontogeneza vrste Centaurium pulchellum (Sw) Druce u kulturi in vitro i analiza sadržaja sekundamih metabolita. Mr thesis. Faculty of Biology, Belgrade; 1998.
Menković, N.; Ŝavikin-Fodulović, K.; Momćilović, I.; Grubiŝié, D. Quantitative determination of secoiridoid and γ-pyrone compounds in Gentiana lutea cultured in vitro. Planta Med. 66:96–98; 2000.
Menkovié, N.; Ŝavikin-Fodulovié, K.; Vinterhalter, B.; Vinterhalter, D.; Grubiŝié, D. Secoiridoid content of naturally grown and in vitro cultured Gentiana punctata. Pharm. Pharmacol. Lett. 8:110–111; 1998.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Schimmer, O.; Mauthner, H. Polymetoxylated xanthones from the herb of Centaurium erythraea with strong antimutagenic properties in Salmonella typhimurium. Planta Med. 62:561–564; 1996.
Ubsdell, R. A. E. Studies on variation and evolution in Centaurium erythraea Rafn., and C. littorale. In: Turner, D., ed. Gilmour in the British Isles. Watsonia 11: 7–31; 1976.
Van der Sluis, W. G. Chemotaxonomical investigation of the genera Blackstonia and Centaurium (Gentianaceae). Plant Syst. Evol. 149:253–278; 1985.
Zeltner, L. Resherches de biosystematique sur les generes Blackstonia et Centaurium. Soc. Neuchatel. Sci. Nat. 93:57–85; 1970.
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Krstić, D., Janković, T., Šavikin-Fodulović, K. et al. Secoiridoids and xanthones in the shoots and roots of Centaurium pulchellum cultured in vitro . In Vitro Cell.Dev.Biol.-Plant 39, 203–207 (2003). https://doi.org/10.1079/IVP2002366
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DOI: https://doi.org/10.1079/IVP2002366