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Shoot regeneration from petioles of coral bells (Heuchera sanguinea Engelm.) cultured in vitro, and subsequent planting and flowering ex vitro


Successful shoot regeneration from petioles, leaves, and petioles with leaves cultured in vitro is reported in Heuchera sanguinea. Petioles or petioles with leaves regenerated more shoots than leaves alone. For culture, the optimum hormonal concentrations were 0.19 μM α-naphthaleneacetic acid combined with 0.44 or 4.4 μM benzyladenine in Murashige and Skoog-based (MS) medium: the regenerating rate and the number of shoots per explant were 60% and 8.6–9.7, respectively. Histological study on petiole culture showed dividing cell clusters including vascular tissues after 1 wk, callus including several dividing cell clusters at the periphery after 3 wk and then apical meristems with immature leaves after 5 wk. Rooting from the regenerated shoots was highest (95%) on MS medium containing 4.9 μM indole-3-butyric acid. Seventy-three percent of rooted plants were successfully acclimatized in pots. When they were cultured in the field, the plants grew and most flowered the following year over winter.

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  • Bailey, L. H.; Bailey, E. Z. Heuchera sanguinea Engelm., In: Bailey, L. H.; Bailey, E. Z., eds. Hortus, 3rd edn. New York: Macmillan Publishing Co; 1976:559–560.

    Google Scholar 

  • Gertsson, U. E. Influence of explant orientation and media composition on organ formation, growth and nitrogen content in petiole explants of Senecio × hybridus Hyl. J. Hort. Sci. 61:121–128; 1986.

    CAS  Google Scholar 

  • Harney, P. M.; Knap, A. A technique for the in vitro propagation of African violets using petioles. Can. J. Plant. Sci. 59:263–266; 1979.

    Article  CAS  Google Scholar 

  • Jensen, W. A. Staining. Delafield's haematoxylin. In: Jensen, W. A., ed. Botanical histochemistry—principles and practice. San Francisco and London: Freeman and Company Press; 1962:90–94.

    Google Scholar 

  • Kitto, S. L.; Frett, J. J.; Geiselhart, P. Micropropagation and field evaluation of × Heucherella ‘Bridget Bloom’. J. Environ. Hort. 8: 156–159; 1990.

    Google Scholar 

  • Lloyd, G.; McCown, B. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip cultures. Comb. Proc. Int. Plant Propagators Soc. 30:421–426; 1981.

    Google Scholar 

  • Miura, Y.; Kitaura, T.; Takayanagi, R.; Mitsuhori, T. Propagation of coral bells (Heuchera sanguinea Engelmann) by tissue culture, and forcing of flowering on the regenerated plants by a combination of low and high temperature treatment. Rep. Kanagawa Pref. Agric. Res. Station 136:17–24; 1995.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Ringe, F.; Nitsch, J. P. Condition leading to flower formation on excised Begonia fragments cultured in vitro. Plant Cell Physiol. 9:639–652; 1968.

    CAS  Google Scholar 

  • Stapfer, R. E.; Heuser, C. W. Rapid multiplication of Heuchera sanguinea Engelm. ‘Rosamundi’ propagated in vitro. HortSci. 21:1043–1044; 1986.

    Google Scholar 

  • Tsukamoto, Y. Heuchera L., In: Tsukamoto, Y., ed. The grand dictionary of horticulture. Tokyo: Shougakkan Press; 1989:401–402.

    Google Scholar 

  • Welander, T. Influence of medium composition on organ formation in vitro explants of Begonia × hiemalis. Swedish J. Agric. Res. 9:163–168; 1979.

    CAS  Google Scholar 

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Correspondence to Takashi Hosoki.

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Hosoki, T., Kajino, E. Shoot regeneration from petioles of coral bells (Heuchera sanguinea Engelm.) cultured in vitro, and subsequent planting and flowering ex vitro . In Vitro Cell.Dev.Biol.-Plant 39, 135–138 (2003).

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Key words

  • Heuchera sanguinea
  • regeneration
  • histology
  • tissue culture