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Propagation of three orchid genera using encapsulated protocorm-like bodies

Summary

Protocorm-like bodies (plbs) of the orchid Dendrobium ‘Sonia’ were obtained from fractionated plb explants on Murashiga and Skoog (1962) medium (MS) supplemented with 4.44 μM 6-benzylaminopurine (BA). The developmental stage of plbs to be encapsulated, concentrations of sodium alginate (2–5%) and calcium chloride (25–100 mM), and concentration of MS salts in the matrix were assessed. Fractionated plbs 13–15 d after culture were at the leaf primordia stage and found suitable for encapsulation studies. The best encapsulation response was observed with 3% sodium alginate upon complexation with 75 mM CaCl2.2H2O. An encapsulation matrix prepared with MS medium (three-quarter strength) supplemented with 0.44 μM BA and 0.54 μM α-naphthaleneacetic acid gave 100% conversion of encapsulated plbs to plants after storage. A viability of >85% of encapsulated plbs of Dendrobium ‘Sonia’ was observed for 75 d at 4°C. Encapsulated plbs of orchids Dendrobium, Oncidium, and Cattleya were stored at 4°C for 75, 60 and 30 d, respectively, with >88% germination. All plants survived potting in media having either wood charcoal pieces alone or in combination with brick pieces.

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Correspondence to G. V. S. Saiprasad.

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Saiprasad, G.V.S., Polisetty, R. Propagation of three orchid genera using encapsulated protocorm-like bodies. In Vitro Cell.Dev.Biol.-Plant 39, 42–48 (2003). https://doi.org/10.1079/IVP2002360

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  • DOI: https://doi.org/10.1079/IVP2002360

Key words

  • artificial seeds
  • synthetic seeds
  • plbs
  • storage temperature
  • duration of storage
  • germination