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Some features of somatic embryo maturation of algerian fir

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Summary

Maturation of Abies numidica De Lann. somatic embryos was tested on media with abseisic acid and various maltose and polyethylene glycol-4000 concentrations. The effect of basal medium and subculture period on maturation was also examined. The maturation of somatic embryos was promoted by polyethylene glycol-4000, at 7.5–10%. Three to 6% maltose significantly enhanced the yield of mature embryos. The most effective somatic embryo maturation occurred when embryogenictissue was transferred to maturation medium after 14–21 d cultivation on proliferation medium. The ability for A. numidica cultures to form cotyledonary somatic embryos was assessed over a period of 3 yr. Plantlets germinated on half-strength SH (Schenk and Hildebrandt) medium with charcoal and survived transfer to the soil.

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Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, SK-950 07, Nitra, Slovak Republic

    Božena Vooková & Andrej Kormuťák

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  1. Božena Vooková
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  2. Andrej Kormuťák
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Correspondence to Božena Vooková.

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Vooková, B., Kormuťák, A. Some features of somatic embryo maturation of algerian fir. In Vitro Cell.Dev.Biol.-Plant 38, 549–551 (2002). https://doi.org/10.1079/IVP2002330

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  • DOI: https://doi.org/10.1079/IVP2002330

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