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In vitro regeneration, flower and plant formation from petiolar and nodal explants of culantro (Eryngium foetidum L.)

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Summary

A procedure for plant regeneration, flower and plant formation from petiolar and inflorescence nodal explants of culantro is discribed. Leaf petioles were excised from young leaves of non-flowering plants while nodal explants were excised from the inflorescence. Explants were cultured in Murashige and Skoog (MS) medium alone or supplemented with 0.5μM naphthaleneacetic acid (NAA) and 0.9, 1.8, 4.5, or 9 μM thidiazuron (TDZ). All explants produced multiple shoots. In addition, nodal explants formed flowers. Shoot number, flower number and shoot length were influenced by TDZ and NAA. Rooted shoots from both types of explants were transferred to soil where plants were successfully established.

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  1. Genetic Engineering and Biotechnology Research Institute, Menoufiya University, P.O. Box, 79/22857, Sadat City, Egypt

    Yasseen Mohamed-Yasseen

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  1. Yasseen Mohamed-Yasseen
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Correspondence to Yasseen Mohamed-Yasseen.

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Mohamed-Yasseen, Y. In vitro regeneration, flower and plant formation from petiolar and nodal explants of culantro (Eryngium foetidum L.). In Vitro Cell Dev Biol -Plant 38, 423–426 (2002). https://doi.org/10.1079/IVP2002311

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  • DOI: https://doi.org/10.1079/IVP2002311

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