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Stage- and sex-dependent changes in the lipid profile of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) during development

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Abstract

Lipids were extracted from the plasma and gonads of fourth and fifth instars and adults of Dysdercus koenigii (Heteroptera: Pyrrhocoridae). Thin layer chromatography of the extracts revealed fractions of free fatty acids (FFA), monoglycerides (MG), diglycerides (DG), triglycerides (TG), phospholipids (PL), free cholesterol (FC) and esterified cholesterol (EC). There were distinct differences in the lipid profiles within sexes for both tissues during development. Phospholipid from female plasma showed significant fluctuation during development, while that from males remained constant. In female gonads, PL levels increased significantly from the fouth instar to the fifth instar. Monoglycerides were detected only in adult male plasma and adult female gonads, at very low levels. Plasma DG in males was found to increase significantly during development. Maximum gonadal DG was recorded in male fourth instars and female fifth instars. Triglycerides in female plasma declined from the fourth instar to the adult stage. In contrast, TG in gonads increased significantly in the fifth instar of both sexes. Plasma FFA of both sexes fluctuated during development. Free cholesterol was detected only in adult male plasma. Plasma EC increased from the fourth instar to the adult stage in males, and it was highest in gonads of fifth instars of both sexes.

Résumé

Des lipides ont été extraits du plasma et des gonades des quatrième et cinquième stades juvéniles et des adultes de Dysdercus koenigii (Heteroptera: Pyrrhocoridae). La Chromatographie sur couche mince des extraits révèle des fractions d’acides gras libres (FFA), des monoglycérides (MG) des diglycérides (DG), des triglycérides (TG), des phospholipides (PL), du cholestérol libre (FC) et du cholestérol esthérifié (EC). Il y a des différences de profils lipidiques selon le sexe pour les deux tissus au cours du développement. Les taux de PL du plasma des femelles présentent des variations significatives pendant le développement alors que ceux des mâles restent constants. Dans les gonades femelles, les taux de PL augmentent significativement entre le quatrième et le cinquième stade juvénile. Les MG ont été détectés uniquement dans le plasma des mâles adultes et dans les gonades femelles à de très faibles taux. Les DG du plasma des mâles augmentent significativement pendant le développement. Les taux maxima de DG dans les gonades ont été trouvés chez les mâles juvéniles de quatrième et cinquième stade. Les taux de TG du plasma des femelles diminuent entre le quatrième stade juvénile et l’adulte. A l’inverse, le taux de TG des gonades augmentent significativement chez le cinquième stade juvénile dans les deux sexes. Les taux de FFA du plasma des deux sexes varient au cours du développement. Les FC sont détectés uniquement dans le plasma des mâles adultes. Le taux de EC du plasma augmente entre le quatrième stade juvénile et les adultes chez les mâles et il est plus élevé dans les gonades du cinquième stade juvénile chez les deux sexes.

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  1. Department of Zoology, Banaras Hindu University, Varanansi, 221005, India

    Archana Mishra, A. K. Singh & Dinesh Kumar

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  1. Archana Mishra
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  2. A. K. Singh
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  3. Dinesh Kumar
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Correspondence to Dinesh Kumar.

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Mishra, A., Singh, A.K. & Kumar, D. Stage- and sex-dependent changes in the lipid profile of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) during development. Int J Trop Insect Sci 24, 236–241 (2004). https://doi.org/10.1079/IJT200428

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