In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 6, pp 534–537

Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis

  • Silvia P. Trajtemberg
  • Nancy M. Apóstolo
  • Graciela Fernández


Cynara cardunculus var. cardunculus L., also known as cardoon, is a perennial weed naturalized in the Pampas region of Argentina. A quantification of cynarine and chlorogenic acid of callus and leaves from cardoon was performed by means of micellar electrokinetic capillary chromatography, showing that the content of cynarine is higher in calluses than in vivo leaves. The scavenging effect of the callus extract, determined by the thiobarbituric acid method, demonstrated its significant antioxidant capacity. The obtained results revealed that in vitro tissue culture is an excellent tool for producing cynarine for therapeutical purposes.

Key words

antixidant activity dicaffeoylquinic acids micellar electrokinetic capillary chromatography (MEKC) monodicaffeoylquinic acids thistle 


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  1. Adzet, T.; Camarasa, J.; Laguna, J. C. Hepatoprotective activity of polyphenolic compounds from Cynara scolymus against CC14 toxicity in isolated rat hepatocytes. J. Nat. Prod. 50(4):612–617; 1987.PubMedCrossRefGoogle Scholar
  2. Ariza Espinar, L.; Delucchi, G. Flora fanerogamica argentina—Asteraceae, Parte II. Tribu Cardueae. Conseio Nacional de Investigaciones Científicas y Tecnológicas (CONICET). Argentina Pro Flora 60:280; 1998.Google Scholar
  3. Ben-hod, G.; Basnizki, Y.; Zohary, D.; Mayer, A. M. Cynarine and chlorogenic acid content in germinating seeds of globe artichoke Cynara scolymus L. J. Genet. Breed. 46:63–68; 1992.Google Scholar
  4. Berregui, I.; Santos, J. I.; Del Campo, G.; Miranda, J. I.; Aizpurua, J. M. Quantitation determination of chlorogenic acid in cider apple juices by 1H NMR spectrometry. Anal. Chim. Acta 486:269–274; 2003.CrossRefGoogle Scholar
  5. Bombardelli, E.; Gabetta, B.; Martinelli, E. M., Gas-liquid chromatography and mass spectrometric investigations on Cynara scolymus L. extracts. Fitoterapia 4:143–151; 1977.Google Scholar
  6. Cordeiro, M.; Xue, Z. T.; Pais, M. S.; Brodelius, P. E. Proteases from cell suspension cultures of Cynara cardunculus. Phytochemistry 33(6):1323–1326; 1993.CrossRefGoogle Scholar
  7. De Fossard, R. A. Tissue culture for plant propagators. Armidale, Australia: Department of Botany, University of New England; 1976.Google Scholar
  8. Debenedetti, S. L.; Palacios, P. S.; Wilson, E. G.; Coussio, J. D. HPLC analysis of caffeoylquinic acids content in Argentine medicinal plants. Acta Hort. (ISHS) 333: 191–200; 1993.Google Scholar
  9. Faro, C. J.; Moir, A. J. G.; Pires, E. V. Specificity and kinetics of the milk clotting enzyme from cardoon (Cynara cardunculus L.) toward bovine K-casein. J. Agric. Food Chem. 41 (10):1537–1540; 1993.CrossRefGoogle Scholar
  10. Figueiredo, A. C.; Fevereiro, P.; Cabral, N. S.; Novais, J. N.; Pais, S. S. Callus and suspension cultures for biomass production of Cynara cardunculus L. (Compositae). Biotechnol. Lett. 9(3):213–218; 1987.CrossRefGoogle Scholar
  11. Gebhardt, R. Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicol. Appl. Pharmacol. 144(2):274–286; 1997.CrossRefGoogle Scholar
  12. Gebhardt, R. Inhibition of cholesterol biosynthesis in primary cultured rat hepatocytes by artichoke (Cynara scolymus L.) extracts. Pharm. Exp. Therap. 286(3):1122–1128; 1998.Google Scholar
  13. Grançal, D.; Nagy, M.; Suchy, V.; Novomesky, P. Cynarine from the fresh flower buds of Cynara cardunculus L. Fitoterapia 65(3):282; 1994.Google Scholar
  14. Inatani, R.; Nagy, M.; Suchy, V.; Novomesky, P. Cynarine from the fresh flower buds of Cynara cardunculus L. Fitoterapia 65(3):282; 1994.Google Scholar
  15. Inatani, R.; Nakatani, N.; Fuwa, H. Antioxidative effect of the constituents of Rosemary Rosmarinus officinalis L. and their derivation. Agric. Biol. Chem. 47(3):521–528; 1983.Google Scholar
  16. Kahl, R.; Kappus, H. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Z. Lebensm. Unters. For. 196:329–338; 1993.CrossRefGoogle Scholar
  17. King, P. J.; Ma, G.; Miaow, W.; Jia, Q.; McDougall, B. R.; Reinecke, M. G.; Cornell, C.; Kuan, J.; Kim, T. R.; Robinson, W. E. Jr. Structure-activity relationships: analogues of the dicaffeoylquinic and dicaffeoyltartaric acids as potent inhibitors of human immunodeficiency virus type 1 integrase replication. J. Med. Chem. 42(3):497–509; 1999.PubMedCrossRefGoogle Scholar
  18. Larson, R. A. The antioxidants of higher plants. Phytochemistry 27(4):969–978; 1988.CrossRefGoogle Scholar
  19. Llorach, R.; Espín, J. C.; Tomás-Barberán, F. A.; Ferreres, F. Artichoke (Cynara scolymus L.) bioproducts as a potential source of health-promoting antioxidant phenolics. J. Agr. Food Chem. 50:3458–3464; 2002.CrossRefGoogle Scholar
  20. Lorenzo, E.; Apóstolo, N. M.; Fernández, G. Cynara cardunculus L. cardo de Castilla: su utilización en la industria quesera y farmacéutica. Anales SAIPA 15:135–142; 1997.Google Scholar
  21. Martino, V. S.; Ferraro, C. E.; Debenedetti, S. L.; Coussio, J. D. Determinación espectrofotométrica del contenido de ácidos cafeoilquinicos en especies argentinas de Compuestas usadas en la medicina popular. Acta Farm. Boanerense 8(1):3–9; 1989.Google Scholar
  22. Mauri, P.; Pietta, P. Electrospray characterization of selected medicinal plants. Pharm. Biom. Anal. 23(1):61–68; 2000.CrossRefGoogle Scholar
  23. Morishita, H.; Iwahashi, H.; Osaka, N.; Kido, R. Chromatographic separation and identification of naturally occuring chlorogenic acids by 1H nuclear magnetic resonance spectroscopy and mass spectrometry. J. Chromatogr. 315:253–260; 1984.PubMedCrossRefGoogle Scholar
  24. Mulinacci, N.; Prucher, D.; Peruzzi, M.; Romani, A.; Pinelli, P.; Giaccherini, C.; Vincieri, F. F. Commercial and laboratory extracts from artichoke leaves: estimation of caffeoyl esters and flavonoidic compounds content. J. Pharm. Biom. Anal. 34:349–357; 2004.CrossRefGoogle Scholar
  25. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–492; 1962.CrossRefGoogle Scholar
  26. Panizzi, L.; Scarpatti, M. L. Isolamento e costituzione del principio attivo del carciofo. Gazzeta Chímica Italiana 84:792–805; 1954.Google Scholar
  27. Pauli, G. F.; Poetsch, F.; Nahrstedt, A. Structure assignment of natural quinic acid derivatives using proton nuclear magnetic resonance techniques. Phytochem. Anal. 9:177–185; 1998.CrossRefGoogle Scholar
  28. Pomilio, A. B.; Trajtemberg, S.; Vitale, A. A. High-Performance Capillary Electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochem. Anal. 13:235–241; 2002.PubMedCrossRefGoogle Scholar
  29. Sevciková, P.; Glatz, Z.; Slanina, J. Analysis of artichoke (Cynana cardunculus L.) extract by means of micellar electrokinetic capillary chromatography. Electrophoresis 23:249–252; 2002.PubMedCrossRefGoogle Scholar
  30. Valentao, P.; Fernandes, E.; Carvalho, F.; Andrade, P. B.; Seabra, R. M.; Bastos, L. Antioxidative properties of cardoon (C. cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. J. Agric. Food Chem. 50:4989–4993; 2002.PubMedCrossRefGoogle Scholar
  31. Wang, M.; Simon, J. E.; Aviles, I. F.; He, K.; Zheng, Q.; Tadmor, Y. Analysis of antioxidative phenolic compounds in artichoke (C. scolymus L.). J. Agric. Food Chem. 51:601–608; 2003.PubMedCrossRefGoogle Scholar
  32. Wiklund, A. The genus Cynara L. (Asteraceae—Cardueae). Bot. J. Lin. Soc. 109:75–123; 1992.Google Scholar
  33. Zhu, X.; Zhang, H.; Lo, R. Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J. Agric. Food Chem. 52:7272–7278; 2004.PubMedCrossRefGoogle Scholar

Copyright information

© Society for In Vitro Biology 2006

Authors and Affiliations

  • Silvia P. Trajtemberg
    • 1
  • Nancy M. Apóstolo
    • 2
  • Graciela Fernández
    • 2
  1. 1.Departmento de Química Orgánica, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos Aires, Ciudad UniversitariaBuenos AiresArgentina
  2. 2.Departmento de Ciencias Básicas, Universidad Nacional de LujánBuenos AiresArgentina

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