Direct shoot and cormlet regeneration from leaf explants of ‘Silk’ banana (AAB)

  • L. Venkatachalam
  • R. Thimmaraju
  • R. V. Sreedhar
  • N. BhagyalakshmiEmail author


Direct shoot and cormlet regeneration from leaf explants were obtained in triploid dessert banana cultivar Nanjanagud Rasabale (NR) that is classified under the group ‘Silk’ and has the genotype AAB. The response for both cormlet and direct shool formation was observed only in leaf explants obtained from shoots cultured in liquid medium but not in similar explants obtained from shoots grown on gelled medium. Shoot initiation occurred after a sequential culture of leaf (sheath) explants on modified Murashige and Skoog (MS) medium supplemented with different growth regulators. In the sequence, the leaf explants were cultured first on medium with a high level (22.4 μM) of benzyladenine (BA), second on indolc-3-butyric acid (IBA) supplemented medium, and third on reduced BA medium under incubation in the dark. The highest adventitious shoot regeneration in 24% of the explants, with the number of shoots ranging from 2 to 3 per explant, occurred in the explants incubated at the first step in medium with 22.4 and 0.198 μM IBA. Further growth and complete shoot formation occurred under incubation in a 16-h photoperiod. While keeping the culture conditions constant and replacing BA with picloram (0.83–20.71 μM) in the initial step, adventious origin of cormlets occurred in 12% of the explants. However, when rhizome explants (also obtained from shoots grown in liquid medium) were cultured with various growth regulators in the first step, medium containing 2,4,5-trichlorophenoxyacctic acid (7.82 μM) produced friable callus that re-differentiated into roots only. Physical forms of the medium, ie.e. agar-gelled or liquid, imparted specific effects on the extent of multiplication of leaf-regenerated shoots with no differences in morphology and growth patterns when compared to those of meristem-derived plants.

Key words

adventitious direct regenerations Musa protocorm triploid 


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  1. Bakry, F.; Rossignol, L. Analyse des capacities de callogenese et d'organogenese obtenues a partir de differents tissue de bananiers (Musa spp. Musacees). Fruits 40:697–708; 1985.Google Scholar
  2. Becker, D. K.; Dugdale, B.; Smith, M. K.; Harding, R. M.; Dale, J. L. Genetic transformation of Cavendish banana (Musa spp AAA group) cultivar ‘Grand Nain’ via microprojectile bombardment. Plant Cell Rep. 19:229–234; 2000.CrossRefGoogle Scholar
  3. Bhagyalakshmi, N.; Singh, N. S. Role of liquid versus agar-gelled media in mass propagation and ex vitro survival in bananas. Plant Cell Tiss. Organ Cult. 41:71–73; 1995.CrossRefGoogle Scholar
  4. Blakesley, D. Uptake and metabolism of 6-benzyladenine in shoot cultures of Musa and Rhododendron. Plant Cell Tiss. Organ Cult. 25:69–74; 1991.CrossRefGoogle Scholar
  5. Cronauer, S.; Krikorian, A. D. Somatic embryos from cultured tissues of triploid plantains (Musa ‘ABB’). Plant Cell Rep. 2:289–291; 1983.CrossRefGoogle Scholar
  6. Cronauer, S.; Krikorian, A. D. Plant regeneration via somatic embyrogenesis in the seeded diploid banana Musa ornata Roxb. Plant Cell Rep. 7:23–25; 1988.CrossRefGoogle Scholar
  7. Dhed'a, D.; Dumortier, F.; Panis, B.; Vuylsteke, D.; De Langhe, E. Plant regeneration in cell suspension cultures of cooking banana ‘Bluggoe’ cultivar (Musa spp ABB group). Fruits 46:125–135; 1991.Google Scholar
  8. Escalant, J. V.; Teisson, C. Compotenments in vitro de embryon isole du bananier (Musa species). Fruits 43:333–342; 1987.Google Scholar
  9. Escalant, J. V.; Teisson, C. Embryogenese somatique chez Musa spp. C.R. Acad. Sci. Paris 306, Ser. III:277–281; 1988.Google Scholar
  10. Escalant, J. V.; Teisson, C.; Cole, F. Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Masa spp.). In Vitro Cell Dev. Biol. Plant 30:181–186; 1994.Google Scholar
  11. Food and Agriculture Organization. FAO Year Book, vol. 57. Geneva: FAO; 2003:185–186.Google Scholar
  12. Ganapathi, T. R.; Srimivas, L.; Suprasama, P.; Bapat, V. A. Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali (Musa spp. AAB group). In Vitro Cell. Dev. Biol. Plant 37:178–181; 2001.CrossRefGoogle Scholar
  13. Harter, L. N. Critical values for Duncan's new multiple range test. Biometrics 16:671–685; 1960.CrossRefGoogle Scholar
  14. Jarret, R. L.; Fisher, J. B.; Litz, R. E. Organ formation in Musa cultures. J. Plant Physiol. 121:123–130; 1985.Google Scholar
  15. Johanson, D. A. Plant Microtechnique. New York: McGraw Hill Book; 1940.Google Scholar
  16. May, C. D.; Afza, R.; Mason, H. S.; Wiecko, A.; Novak, F. J.; Arntzen, C. J. Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation. Bio-Technology 13:486–492; 1995.Google Scholar
  17. Montgomery, C. D. Design and analysis of experiments. New York: John Wiley & Sons; 1976.Google Scholar
  18. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473–497; 1962.CrossRefGoogle Scholar
  19. Navarro, C.; Escobedo, R. M.; Mayo, A. In vitro plant regeneration from embryonic cultures of a diploid and a triploid. Cavendish banana. Plant Cell Tiss. Organ Cult. 51:17–25; 1997.CrossRefGoogle Scholar
  20. Novak, E. J.; Afza, R.; Van Duren, M.; Perea-Dallos, M.; Conger, B. V.; Xiaolang, T. Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking bananas (Musa spp.). Bio-Technology 7:154–159; 1989.Google Scholar
  21. Okole, B. N.; Schulz, F. A. Micro-cross sections of banana and plantains (Musa spp.): morphogenesis and regeneration of callus and shoot buds. Plant Sci. 116:185–195; 1996.CrossRefGoogle Scholar
  22. Panis, B.; Swennen, R. Cultures cellaires embryogenes de Musa: applications actuelles et futures. Informusa 2:3–6; 1993.Google Scholar
  23. Shi, C. T.; Ma, S. S.; Huang, I. C.; Ching, W. H. Somatic embryogenesis and plantlet regeneration in suspension cell cultures of triploid bananas (Musa AAA) subgroup Cavendish. In International symposium on Recent Development in Banana Cultivation Technology, 14–18 December, Taiwan: Bananas Research Institute; 1992:12–21.Google Scholar
  24. Wealth of India. Raw material, vol. 6 (L-M). New Delhi: CSIR; 1962.Google Scholar

Copyright information

© Society for In Vitro Biology 2006

Authors and Affiliations

  • L. Venkatachalam
    • 1
  • R. Thimmaraju
    • 1
  • R. V. Sreedhar
    • 1
  • N. Bhagyalakshmi
    • 1
    Email author
  1. 1.Plant Cell Biotechnology DepartmentCentral Food Technological Research InstituteIndia

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