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In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 5, pp 422–426 | Cite as

Manipulation by culture mixing and elicitation of paclitaxel and baccatin III production in Taxus baccata suspension cultures

  • M. Bonfill
  • O. Expósito
  • E. Moyano
  • R. M. Cusidó
  • J. Palazón
  • M. T. Piñol
Article

Summary

Experiments were carried out with Taxus baccata cell lines showing different paclitaxel-producing capacities (between 1.74 and 19.91 mgl−1) when growing in a selected product-formation medium that specifically stimulated the production of taxane to the detriment of cell growth. Through mixing low-, medial- and high-producing lines, it could be observed that paclitaxel productivity in the resulting mixed lines was clearly higher than the mean productivity of the individual lines before mixing. This suggests that culture components generated by high-producing individual lines within the population might induce paclitaxel production. Although the accumulation of paclitaxel and baccatin III was higher when 100 μM methyl jasmonate was added to the subcultures of the mixed lines, the results indicate that exogenously applied methyl jasmonate was not the first factor to stimulate taxane production. The possible effects of methyl jasmonate elicitation and paclitaxel accumulation on cell viability are also considered.

Key words

cell line mixing cell viability methyl jasmonate taxanes Taxus species 

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References

  1. Cusidó, R. M.; Palazón, J.; Bonfill, M.; Navia-Osorio, A.; Morales, C.; Piñol, M. T. Improved paclitaxel and baccatin III production in suspension cultures of Taxus media. Biotechnol. Prog. 18:418–423; 2002.CrossRefGoogle Scholar
  2. Cusidó, R. M.; Palazón, J.; Navia-Osorio, A.; Bonfill, M.; Morales, C.; Piñol, M. T. Production of taxol and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture. Plant Sci. 146:101–107; 1999.CrossRefGoogle Scholar
  3. Farmer, E. E.; Ryan, C. A. Interplant communication: airborne methyl jasmonates induces synthesis of proteinase inhibitors in plant leaves. Proc. Natl. Acad. Sci. USA 87:7713–7716; 1990.PubMedCrossRefGoogle Scholar
  4. Fett-Neto, A. G.; DiCosmo, F. Taxol and taxane production by cell culture In: Encyclopedia of molecular biology and molecular medicine, vol. 6. Weinheim, Germany: VCH, Verlags-gesllschaft; 1997:10–17.Google Scholar
  5. Fleming, P. E.; Knaggs, A. R.; He, X. G.; Mocek, U.; Floss, H. G. Biosynthesis of taxoids. Mode of attachment of taxol side chain. J. Am. Chem. Soc. 116:4137–4138; 1994.CrossRefGoogle Scholar
  6. Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50:151–158; 1968.PubMedCrossRefGoogle Scholar
  7. Grothaus, P. G.; Bignami, G. S.; O'Malley, S.; Harada, K. E.; Byrnes, J. B.; Walle, D. F.; Raybould, T. J. G.; MacGuire, M. T.; Alvarado, B. Taxane-specific monoclonal antibodies: measurement of taxol, baccatin III, and total taxanes in Taxus brevifolia extracts by enzyme immunoassay. J. Nat. Prod. 58:1003–1014; 1995.PubMedCrossRefGoogle Scholar
  8. Guénard, D.; Guéritte-Voegelein, F.; Potier, P. Taxol and taxotere: discovery, chemistry and structure-activity relationships. Acc. Chem. Res. 26:160–167; 1993.CrossRefGoogle Scholar
  9. Hall, R. D.; Holden, M. A.; Yeoman, M. M. Immobilization of higher plant cell. In: bajaj, Y. P. S. ed. Biotechnology in agriculture and forestry, vol. 4. Berlin, Heidelberg: Springer Verlag: 1988:136–156.Google Scholar
  10. Hirasuna, T. J.; Pestchanker, L. J.; Srinivasan, V.; Shuler, M. L. Taxol production in suspension cultures of Taxus baccata. Plant Cell Tiss. Organ Cult. 44:95–102; 1996.CrossRefGoogle Scholar
  11. Holden, P. R.; Holden, M. A.; Yeoman, M. M. Variation in the secondary metabolism of cultured plant cells. In: Robins, R. J.; Rhodes, M. J. C., eds. Manipulating secondary metabolism in culture. Cambridge: Cambridge University Press; 1988:15–29.Google Scholar
  12. Jordon, M. A.; Wilson, L. Microtuble polymerisation dynamics, mitotic block, and cell death by paclitaxel at low concentrations. In: Georg, G. I.; Chen, T. T.; Ojima, I.; Vyas, D. M., eds. Taxane anticancer agents. Washington, DC: American Chemical Society; 1995:138–153.Google Scholar
  13. Kawamura, M.; Shigeoka, T.; Tahara, M.; Tamaki, M.; Ohashi, H.; Akita, M.; Kobayashi, Y.; Sakamoto, T. Efficient selection of cells with high taxol content from heterogeneous Taxus cell suspensions by magnetic or fluorescent antibodies. Seibutsu-kogaku 76:3–7; 1998.Google Scholar
  14. Ketchum, R. E. B.; Gibson, D. M. Paclitaxel production in suspension cell cultures of Taxus. Plant Cell Tiss. Organ Cult. 46: 9–16; 1996.Google Scholar
  15. Ketchum, R. E. B.; Gibson, D.; Croteau, R.; Shuler, M. L. The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62:97–105; 1999.PubMedCrossRefGoogle Scholar
  16. Ketchum, R. E. B.; Rithner, C. D.; Qiu, D.; Kim, Y. S.; Williams, R. M.; Croteau, R. B. Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus media cell cultures. Phytochemistry 62:901–909; 2003.PubMedCrossRefGoogle Scholar
  17. Kim, B. J.; Gibson, D. M.; Shuler, M. L. Effect of subculture and elicitation on instability of taxol production in Taxus sp. suspension cultures. Biotechnol. Prog. 20:1666–1673; 2004.PubMedCrossRefGoogle Scholar
  18. Kim, J. H.; Yun, J. H.; Hwang, Y. S.; Byun, S. Y.; Kim, D. I. Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Biotechnol. Lett. 17:101–106; 1995.CrossRefGoogle Scholar
  19. Palazón, J.; Cusidó, R. M.; Bonfill, M.; Morales, C.; Piñol, M. T. Inhibition of paclitaxed and baccatin III accumulation by mevinolin and fosmidomycin in suspension cultures of Taxus baccata. J. Biotechnol. 101:157–163; 2003.CrossRefGoogle Scholar
  20. Rhodes, M. J. C.; Hamill, J.; Parr, A. J.; Robins, R. J.; Walton, N. J. Strain improvement by screening and selection techniques. In: Robins, R. J.; Rhodes, M. J. C. eds. Manipulating secondary metabolism in culture. Cambridge: Cambridge University Press; 1988:83–93.Google Scholar
  21. Ritter, S. K. Green innovations. Chem. Eng. News 82:25–30; 2004.Google Scholar
  22. Seki, M.; Ohzora, C.; Takeda, M.; Furusaki, S. Taxol (paclitaxel) production using free and immobilized cells of Taxus cuspidate. Biotechnol. Bioeng. 53:214–219; 1997.CrossRefGoogle Scholar
  23. Srinivasan, V.; Ciddi, V.; Bring, V.; Shuler, M. L. Metabolic inhibitors, elicitors and precursors as tools for probing yield limitation in taxane production by Taxus chinensis cell cultures. Biotechnol. Prog. 12:457–465; 1996.PubMedCrossRefGoogle Scholar
  24. Tabata, H. Paclitaxel production by plant-cell-culture technology. Adv. Biochem. Eng. Biotechnol. 87:1–23; 2004.PubMedGoogle Scholar
  25. Widholm, J. M. The use of fluorecein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47:189–194; 1972.PubMedGoogle Scholar
  26. Yukimune, Y.; Tabata, H.; Higashi, Y.; Hara, Y. Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat. Biotechnol. 14:1129–1132; 1996.PubMedCrossRefGoogle Scholar

Copyright information

© Society for In Vitro Biology 2006

Authors and Affiliations

  • M. Bonfill
    • 1
  • O. Expósito
    • 1
  • E. Moyano
    • 2
  • R. M. Cusidó
    • 1
  • J. Palazón
    • 1
  • M. T. Piñol
    • 1
  1. 1.Sección de Fisiología Vegetal, Facultad de FarmaciaUniversidad de BarcelonaBarcelonaSpain
  2. 2.Department de Ciències Experimentals i de la SalutUniversitat Pompeu FabraBarcelonaSpain

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