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In Vitro Cellular & Developmental Biology - Plant

, Volume 41, Issue 6, pp 765–769 | Cite as

Cytokinins induce direc somatic embryogenesis of Dendrobium chiengmai pink and subsequent plant regeneration

  • Hsiao-Hang Chung
  • Jen-Tsung Chen
  • Wei-Chin ChangEmail author
Article

Summary

Four auxins (2,4-dichlorophenoxyacetic acid [2,4-D], indole-3-acetic acid [IAA], indole-3-butyric acid [IBA], and naphthaleneacetic acid [NAA]), and five cytokinins (N 6-[2-isopentenyl]-adenine [2iP], N 6-benzyladenine [BA], 6-furfurylaminopurine [kinetin], 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea [TDZ], and 6-[4-hydroxy-3-methylbut-2-enylamino]purine [zeatin]) were examined for their effects on direct embryo induction from leaf explants of Dendrobium cv. Chiengmai Pink cultured on 1/2 Murashige and Skoog (MS) medium. Whether in light or darkness, explants easily became necrotic and no embryos were obtained on growth regulator-free or auxin-containing media after 60 d of culture. By contrast, five cytokinins tested induced direct embryo formation from leaf explants, and explants cultured in light had a higher embryogenic response compared with those cultured in darkness. The best condition for direct embryo induction was at 18.16 μM TDZ cultured in light for 60 d, where 33% of explants formed a mean number of 33.6 embryos per explant. During subculture on growth regulator-free 1/2 MS medium, embryos gradually developed into plantlets. Secondary embryogenesis was occasionally found on sheath leaves of embryos. Regenerated plantlets were successfully transplanted and grown in a greenhouse environment.

Key words

cytokinin direct somatic embryogenesis leaf explant 

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Copyright information

© Society for In Vitro Biology 2005

Authors and Affiliations

  • Hsiao-Hang Chung
    • 1
  • Jen-Tsung Chen
    • 1
  • Wei-Chin Chang
    • 1
    Email author
  1. 1.Institute of Plant and Microbial Biology BotanyAcademia SinicaTaipeiTaiwan, Republic of China

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