In Vitro Cellular & Developmental Biology - Plant

, Volume 41, Issue 5, pp 666–670 | Cite as

In vitro propagation of the chinese medicinal plant, Dendrobium candidum wall. ex lindl., from axenic nodal segments

  • Yih-Juh Shiau
  • Satish Manohar Nalawade
  • Chi-Ni Hsia
  • Vanisree Mulabagal
  • Hsin-Sheng TsayEmail author


Dendrobium candidum Wall. Ex Lindl. is an important species used in the formulation of Shih-hu, a Chinese traditional medicine. An efficient protocol for in vitro propagation of D. candidum using the axenic nodal segments of the shoots, originated from the in vitro germinated seedlings, was developed. The seeds from 120-d-old capsules after pollination were first germinated on half-strength Murashige and Skoog (MS) basal medium supplemented with 30 g l−1 sucrose. After 4 mo., the seedlings were subcultured on a similar medium supplemented with 1 ml l−1 HYPONeX, 80 g l−1 potato homogenate and 2 g l−1 activated charcoal for further growth. Axenic nodal segments excised from 9-mo.-old seedlings were cultured on the medium in the presence of 2 mg l−1 benzyladenine (BA) and 0.1 mg l−1 naphthaleneacetic acid (NAA). After 75 d, 73.2% of the explants gave rise to buds/shoots. The elongated shoots were rooted on the medium containing 0.2 mg l−1 NAA and the plantlets were successfully acclimatized in soil.

Key words

axenic nodal segments Dendrobium candidum medicinal herb tissue culture 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anonymous. Dendrobium candidum Wall. ex Lindl. In: Hu, S. M., ed. Chung-Hua-Ben-Tsao, Shanghai, China: Shanghai Science Technology Press; 1999:705–711 (in Chinese).Google Scholar
  2. Fay, M. F. Conservation of rare and endangered plants using in vitro methods. In Vitro Cell. Dev. Biol.-Plant 28:1–4; 1992.Google Scholar
  3. Fu, F. C.; Lian, S. C.; Feng, D. Q.; Zhang, S. Y. The development and use of Dendrobium candidum Wall. ex. Lindl. natural resources. Chin. Trad. Drugs 30:708–711; 1999 (in Chinese).Google Scholar
  4. George, E. F. Plant propagation by tissue culture. Part 1: The technology. Edington: Exgetics Ltd.; 1993:3–36.Google Scholar
  5. Guo, S. X.; Cao, W. Q.; Xu, J. T. Studies on the preparation process and germination of white Dendrobium candidum artificial seeds. Chin. Trad. Drugs 27:105–107; 1996 (in Chinese).Google Scholar
  6. Hawkes, A. D. Dendrobium candidum Wall. ex. Ldl.. In: Hawkes, A., ed. Encyclopedia of cultivated orchids, an illustrated, descriptive manual of the members of the Orchidaceae currently in cultivation. Whitstable: Latimer Trend & Co. Ltd.; 1970:153–154.Google Scholar
  7. Hu, S. Y. Dendrobium in Chinese medicine. Econ. Bot. 24:165–170; 1970.Google Scholar
  8. Huang, M. Q.; Lu, Y. J. Prospect on the callus culture of Dendrobium candidum used as drugs. J. Chin. Herb. Drugs 21:543–545; 1998 (in Chinese).Google Scholar
  9. Huang, M. Q.; Ruan, J. Y. Analysis and determination of amino acids in Dendrobium candidum. J. Clin. Herb. Drugs 20:32–33; 1997 (in Chinese).Google Scholar
  10. Jones, W. E.; Kuehnle, A. R.; Arumuganathan, K. Nuclear DNA content of 26 orchids (Orchidaceae) genera with emphasis on Dendrobium. Ann. Bot. 82:189–194; 1998.CrossRefGoogle Scholar
  11. Kanjilal, B.; Sarker, D. De; Mitra, J.; Datta, K. B. Stem disc culture: development of a rapid mass propagation method for Dendrobium moschatum (Buch-Ham) Swartz: an endangered orchid. Curr. Sci. 77:497–500; 1999.Google Scholar
  12. Kukulczanka, K.; Wojciechowska, U. Propagation of two Dendrobium species by in vitro culture. Acta Hort. 131:105–110; 1983.Google Scholar
  13. Lau, D. T. W.; Shaw, P. C.; Wang, J.; But, P. P. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA. Planta Med. 67:456–460; 2001.PubMedCrossRefGoogle Scholar
  14. Lin, T. P. Dendrobium moniliforme (L.) Sw. Native orchids of Taiwan. Taipei, ROC: Southern Materials Center, Inc.; 1975:136–137 (in Chinese).Google Scholar
  15. Liu, C. Z.; Murch, S. J.; El-Demerdash, M.; Saxena, P. K. Artemisia judaica L.: Micropropagation and antioxidant activity. J. Biotechnol. 110:63–71; 2004.PubMedCrossRefGoogle Scholar
  16. Liu, H.; Zhang, Z. G. Studies on seedling development medium of Dendrobium candidum Wall. ex. Lindl. China J. Chin. Materia Med. 23:654–658; 1998 (in Chinese).Google Scholar
  17. Liu, R. J.; Meng, A. D.; Deng, X. Q.; Li, Y. R. Seedling rapid propagation of Dendrobium candidum. Acta Pharm. 23:636–640; 1988.Google Scholar
  18. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.CrossRefGoogle Scholar
  19. Nasiruddin, K. M.; Begum, R.; Yasmin, S. Protocorm like bodies and plantlet regeneration from Dendrobium formosum leaf callus. Asian J. Plant Sci. 2:955–957; 2003.CrossRefGoogle Scholar
  20. Nayak, N. R.; Rath, S. P.; Patnaik, S. In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci. Hort. 71:243–250; 1997.CrossRefGoogle Scholar
  21. Nayak, N. R.; Sahoo, S.; Patnaik, S.; Rath, S. P. Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Sci. Hort. 94:107–116; 2002.CrossRefGoogle Scholar
  22. Pierik, R. L. M.; Sprenkels, P. A.; Van der Harst, B.; Van der Meys, Q. G. Seed germination and further development of Paphiopedilum ciliolare Pfitz. in vitro. Sci. Hort. 34:139–153; 1988.CrossRefGoogle Scholar
  23. Shiau, Y. J.; Sagare, A. P.; Chen, U. C.; Yang, S. R.; Tsay, H. S. Conservation of Anoectochilus formosanus Hayata by artificial crosspollination and in vitro culture of seeds. Bot. Bull. Acad. Sin. 43:123–130; 2002.Google Scholar
  24. Tang, W.; Eisenbrand, G. Dendrobium nobile Lindl. In: Tang, W.; Eisenbrand, G., eds. Chinese drugs of plant origin chemistry, pharmacology, and use in traditional and modern medicine. Berlin Heidelberg: Springer-Verlag; 1992:451–454.Google Scholar
  25. Tripepi, R. R. Adventitious shoot regeneration. In: Geneve, R. L.; Preece, J. E.; Merkle, S. A., eds. Biotechnology of ornamental plants—biotechnology in agriculture series, No 16. Wallingford: CAB International; 1997:45–71.Google Scholar
  26. Wang, G. Y.; Xu, Z. H.; Chia, T. F.; Chua, N. M. In vitro flowering of orchid (Dendrobium candidum) In: You, C. B., ed. Biotechnology in agriculture. Netherlands: Kluwer Academic Publishers; 1993:373–378.Google Scholar
  27. Withner, C. L. List of chromosome numbers in species of the Orchidaceae. In: Withner, C. L., ed. The orchids scientific studies. Toronto: John Wiley & Sons, Inc.: 1974:430.Google Scholar
  28. Yang, J.; Lee, H. J.; Shin, D. H.; Oh, S. K.; Seon, J. H.; Paek, K. Y.; Han, K. H. Genetic transformation of Cymbidium orchid by particle bombardment. Plant Cell Rep. 18:978–984; 1999.CrossRefGoogle Scholar
  29. Yen, K. Y. Dendrobii caulis In: Yen, K. Y., ed. The illustrated Chinese Materia Medica prepared drugs. Taipei: S.M.C. Publishing Inc.; 1980:11–12 (in Chinese).Google Scholar
  30. Yi, H. F.; Qi, X. J.; Zhou, W. T.; Yue, H. Prospect seedling rapid development, ex vitro establishment of Dendrobium candidum and determination of polysaccharide. Chin. Trad. Patent. Med. 21:658–659; 1999 (in Chinese).Google Scholar
  31. Zeng, S. G.; Cheng, S. J. Tissue culture and rapid propagation of Dendrobium. Chin. Trad. Drugs 19:490–491; 1996 (in Chinese).Google Scholar
  32. Zhang, Z. G.; Liu, H.; Wang, L.; Cai, Z. G.; Han, X. Z.; Zhao, L. H.; Luo, Z. J. Studies on protocorm propagation of Dendrobium candidum Wall. ex. Lindl. J. Chin. Herb. Drugs 23:431–434; 1992 (in Chinese).Google Scholar
  33. Zhang, Z. G.; Wang, L.; Liu, H.; Cai, Z. G.; Han, X. Z.; Zhao, L. H.; Luo, Z. J. Studies on protocorm differentiation medium of Dendrobium candidum Wall. ex. Lindl. China J. Chin. Materia Med. 18:16–19; 1993 (in Chinese).Google Scholar

Copyright information

© Society for In Vitro Biology 2005

Authors and Affiliations

  • Yih-Juh Shiau
    • 1
  • Satish Manohar Nalawade
    • 2
  • Chi-Ni Hsia
    • 1
  • Vanisree Mulabagal
    • 1
  • Hsin-Sheng Tsay
    • 2
    Email author
  1. 1.Department of AgronomyAgricultural Research InstituteTaichungTaiwan
  2. 2.Institute of BiotechnologyChaoyang University of TechnologyTaichungTaiwan

Personalised recommendations