Summary
Effects of genotype and explant orientation on shoot regeneration from cotyledonary explants of tomato were studied using 10 commercially important cultivars. The explant orientation affected shoot regeneration in all the tested genotypes. Cotyledons placed in abaxial (lower surface facing down) orientation consistently produced better shoot regenerative response and produced greater numbers and taller shoots compared to those inoculated in adaxial (upper surface facing down) orientation. Genotypic variation in terms of shoot regeneration response, number of shoots, and shoot height was apparent. Wounding of cotyledonary explants increased shoot regeneration response. However, shoot height was much lower in shoots regenerated from wounded explants compared to those that originated from intact cotyledons. Shoots produced from wounded cotyledons were abnormal in their form and structure.
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Bentz, S. E.; Parliman, B. J.; Talbott, H. J.; Ackerman, W. L. Factors affecting in vitro propagation of Yucca glauca. Plant Cell Tiss. Organ Cult. 14:111–120; 1988.
Bhatia, P. Regeneration, micropropagation and somatic embryogenesis in tomato (Lycopersicon esculentum Mill.), Ph.D. thesis. Central Queensland University, Rockhampton, Australia: 2004.
Bhatia, P.; Ashwath, N.; Senaratna, T.; Midmore, D. Tissue culture studies of tomato (Lycopersicon esculentum). Plant Cell Tiss. Organ Cult. 78:1–21: 2004.
Browning, G.; Ognjanov, V.; Passey, A. J.; James, D. J. Multiple shoot and root regeneration from pear embryo cotyledon explant in vitro. J. Hort. Sci. 62:305–311; 1987.
Callow, P.; Haghighi, K.; Giroux, M.; Hancock, J. In vitro shoot regeneration on leaf tissue from micropropagated high bush blueberry. HortScience 23:807–808; 1988.
Costa, M. G. C.; Nogueira, F. T. S.; Figueira, M. L.; Otoni, W. C.; Brommonschenkel, S. H.; Cecon, P. R. Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. 19:327–332; 2000a.
Costa, G. M.; Nogueira, F. T. S.; Otoni, W. C.; Brommonschenkel, S. H. In vitro regeneration of processing tomato (Lycopersicon esculentum Mill.) ‘IPA-5’ and ‘IPA-6’. Ciencia Agrotecnol. 24:671–678; 2000b.
Duzyaman, E.; Tanrisever, A.; Gunver, G. Comparative studies on regeneration of different tissues of tomato in vitro. Acta Hort. 366:235–242; 1994.
El-Farash, E. M.; Abdalla, H. I.; Taghian, A. S.; Ahmad, M. H. Genotype, explant age and explant type as affecting callus and shoot regeneration in tomato. Assiut J. Agric. Sci. 24:3–14; 1993.
Frankenberger, E. A.; Hasegawa, P. M.; Tigchelaar, E. C. Influence of environment and developmental state on the shoot-forming capacity of tomato genotypes. Z. Pflanzenphysiol. 102:221–232; 1981.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–168; 1968.
George, E. F. Factors affecting growth and morphogenesis. In: George, E. F., ed. Plant propagation by tissue culture. London: Exegetics Ltd.; 1993;231–271.
Gorbatenko, I. Y. Micropropagation of different tomato genotypes in vitro. Doklady Vsesoyuznoi Ordena Lenina i Ordena Trudovogo Krasnogo Znameni Akademii Sel'skokhozyaistvennykh Nauk Imeni 18–22; 1990.
Korban, S. S.; Skirvin, R. M. In vitro shoot regeneration from an intact and a sectioned embryo-axis of apple seeds. Plant Sci. 39:61–66; 1985.
Kurtz, S. M.; Lineberger, R. D. Genotypic differences in morphogenic capacity of cultured leaf explants of tomato. J. Am. Soc. Hort. Sci. 108:710–714; 1983.
Mariotti, D.; Arcioni, S. Coronilla varia L. (Crown vetch): plant regeneration through somatic embryogenesis. Plant Cell Tiss. Organ Cult. 2:103–110; 1983.
Mauseth, J. D.; Halperin, W. Hormonal control of organogenesis in Opuntia polyacantha (Cactaceae). Am. J. Bot. 62:869–877; 1975.
McCullagh, P.; Nelder, J. Generalised linear models, New York: Chapman and Hall; 1983.
Murashige, T., Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Niederwieser, J. G.; Veelar, B. M. Regeneration of Lachenalia species from leaf explants. HortScience 25:684–687; 1990.
Padmanabhan, V.; Paddock, E. F.; Sharp, W. R. Plantlet formation in vitro from Lycopersicon esculentum leaf callus. Can. J. Bot. 52:1429–1432; 1974.
Park, Y. G.; Son, S. H. In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra × P. maximowiezii. Plant Cell Tiss. Organ Cult. 15:95–105; 1988.
Payne, R. Analysis of variance for unbalanced design. In: Payne, R.; Arnold, G., eds. Procedure library PL14, GenStat® release 6.1 reference manual. Oxford: VSN International Ltd.; 2002:69.
Plastira, V. A.; Perdikaris, A. K. Effect of genotype and explant type on regeneration frequency of tomato in vitro. Acta Hort. 447:231–234; 1997.
Stommel, J. R.; Sinden, S. L. Genotypic differences in shoot-forming capacity of cultured leaf explants of Lycopersicon hirsutum. HortScience 26:1317–1320; 1991.
Tal, M.; Dehan, K.; Heikin, H. Morphogenetic potential of cultured leaf sections of cultivated and wild species of tomato. Ann. Bot. 41:937–941; 1977.
Venkatachalam, P.; Geetha, N.; Priya, P.; Rajaseger, G.; Jayabalan, N. High frequency plantlet regeneration from hypocotyl explants of tomato (Lycopersicon esculentum Mill.) via organogenesis. Plant Cell Biotechnol. Mol. Biol. 1:95–100; 2000.
Welander, M. J. Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. Plant Physiol. 132:738–744; 1988.
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Bhatia, P., Ashwath, N. & Midmore, D.J. Effects of genotype, explant orientation, and wounding on shoot regeneration in tomato. In Vitro Cell.Dev.Biol.-Plant 41, 457–464 (2005). https://doi.org/10.1079/IVP2005649
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DOI: https://doi.org/10.1079/IVP2005649