Guar gum as a gelling agent for plant tissue culture media
- 405 Downloads
Guar gum, a galactomannan derived from the endosperms of Cyamposis tetragonoloba, has been successfully used as a sole gelling agent for plant tissue culture media. Its suitability as a gelling agent was demonstrated by using guar gumgelled media for in vitro seed germination of Linum usitatissimum and Brassica juncea, in vitro axillary shoot proliferation in nodal explants of Crataeva nurvala, rooting of regenerated shoots of the same, in vitro androgenesis in anther cultures of Nicotiana tabacum, and somatic embryogenesis in callus cultures of Calliandra tweedii. The media used for these were gelled with either guar gum (2, 3, or 4%) or agar (0.9%). Guar gum-gelled media, like agar media, supported all these morphogenic responses. Rather, axillary shoot proliferation, rhizogenic and embryogenic responses were better on guar gum-gelled media than on agar media.
Key wordsandrogenesis axillary shoot proliferation embryogenesis rhizogenesis seed germination
Unable to display preview. Download preview PDF.
- Anonymous, Gums, food resources, http://food.orst.edu/; 2001.Google Scholar
- Chu, C. C. The N6 medium and its applications to anther culture of cereal crops In: Proc. Symp. Plant Tiss. Cult., Beijing: Science Press; 1978:43–59.Google Scholar
- Creager, J. G.; Black, J. G.; Davison, V. E. Microbiology: principles and applications. Englewood Cliffs, NJ: Prentice Hall; 1990.Google Scholar
- Eyre, M. J.; Caswell, S. C. Sterile culture of Rotylenchulus reniformis on tomato root with gellan gum as a supporting medium. J. Nematol. 23:229–231; 1991.Google Scholar
- Jain, R.; Babbar, S. B. Abstract. Proc. 4th Int. Plant Tiss. Cult. Conf. Dhaka; 2001:97.Google Scholar
- Knop, W. Quantitative Untersuchung Über die Ernährungsprozesse der Pflanzen. Landwirtsch Vers. Stn. 30:292–294; 1865.Google Scholar
- Nitsch, J. P. Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404; 1969.Google Scholar
- Pelezar, M. J. Jr.; Chan, E. C. S.; Krieg, N. R. Microbiology. Singapore: McGraw-Hill Book Co.; 1986.Google Scholar
- Redenbaugh, K.; Fujii, J. A. A.; Slade, D. Hydrated coatings for synthetic seeds. In: Redenbaugh, K., ed. Synseeds: applications of synthetic seeds to crop improvement. Boca Raton, FL: CRC Press; 1993:35–46.Google Scholar
- Sunderland, N.; Dunwell, J. M. Anther and pollen culture. In: Street, H. E., ed. Plant tissue and cell culture. Oxford: Blackwell Scientific Publishers; 1977:223–264.Google Scholar
- Windholz, M.; Budavari, S.; Blumetti, R. F.; Otterbein, E. S. The Merck Index: an encyclopedia of chemicals, drugs and biologicals, 3rd edn. Rahway, NJ: Merck and Co. Inc.: 1983.Google Scholar