Leaf anatomy of Cynara scolymus L. in successive micropropagation stages
Leaf structure along the successive stages of Early French artichoke Cynara scolymus L. micropropagation was characterized using light and transmission electron microscopy. The mesophyll presents disorganized spongy and palisade parenchyma with large intercellular spaces and a few small chloroplasts in the leaves of plants cultured in vitro. In addition, both epidermal surfaces of such leaves invariably show a cell wall of the same thickness with a very thin cuticle and open stomata. In the root differentiation stage in vitro, structural changes take place in the leaves that are favorable for survival in the acclimatization stage: conspicuous cuticle, greater cell wall thickness, functional stomata, better mesophyll organization, developed vascular bundles, and the presence of sclerenchymatous tissue are observed. These features found in later in vitro stages are maintained in the following ex vitro stages, some becoming more evident. Our results demonstrate that the structural changes required to ensure appropriate acclimatization of micropropagated artichoke plants begin at the root differentiation stage, which can reduce in vivo acclimatization time and achieve greater survival of transferred plants.
Key wordsartichoke in vitro culture mesophyll stomata
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- Apóstolo, N. M.; Brutti, C. B.; Ferrarotti, S. A.; Llorente, B. E.; Krymkiewicz, N. Stimulation of root development with cyclodextrins on jojoba shoots in vitro. In Vitro Cell. Dev. Biol. Plant 37:404–418; 2001.Google Scholar
- Donnelly, D. J.; Skelton, F. E.; Daubeny, H. A. External leaf features of tissue-cultured Silvan blackberry. HortScience 21:306–308; 1986.Google Scholar
- Donnelly, D. J.; Tisdall, L. Acclimatization strategies for micropropagated plants. In: Ahuja M. R., ed. Micropropagation of woody plants. Dordrecht: Kluwer Academic Publishers; 1993:153–156.Google Scholar
- Freeman, B.; Albrigo, L. G.; Briggs, B. H. Cuticular waxes of developing leaves and fruit of blueberry Vaccimium ashei Reade cv. Bluegem. J. Am. Soc. Hort. Sci. 194:398–403; 1979.Google Scholar
- Heller, R. Recherches sur la nutrition minerales des tissues végétaux cultives in vitro. Ann. Sci. Nat. Bot. Biol. Veg. 14:1–223; 1953.Google Scholar
- Kozai, T.; Fujiwara, M.; Nayashi, J.; Aitken-Christie, J. The in vitro environment and its control in micropropagation. In: Kurata, K.; Kozai, T., eds Transplant production systems. Dordrecht: Kluwer Academic Publishers; 1992:247–282.Google Scholar
- Noc, N.; Bonini, L. Leaf anatomy of highbush blucberry grown in vitro and during acclimatization to ex vitro conditions. Biol. Plant. 38:19–25; 1996.Google Scholar
- Rossi, V.; De Paoli, G. Micropropagation of artichoke (Cynara scolymus L.). In: Bajaj Y. P. S., ed. Biotechnology in agriculture and forestry: high-tech and micropropagation II, vol. 19, Berlin: Springer-Verlag; 1992:118–134.Google Scholar
- Sha Valli Khan P. S.; Evers, D.; Hausman, J. F. Stomatal characteristics and water relations of in vitro grown Quercus robur NL 100 in relation to acclimatization. Silvac Genet. 48:83; 1999.Google Scholar
- Willmer, C. M. Los estomas [The stomata]. Buenos Aires: Edit. Librería Agropecuaria; 1986.Google Scholar