Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort.
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Direct plant regeneration from flowering plant-derived lamina explants of Anthurium andraeanum Hort. cultivars Tinora Red and Senator was established on modified Murashige and Skoog (MS) medium. Cultivar difference, stage of source lamina and the position of explant in lamina, medium pH, and type of growth regulators significantly influenced direct plant regeneration. Explants from young brown lamina were superior to young green lamina. The half-strength MS medium containing 1.11 μM N6-benzyladenine (BA), 1.14 μM indole-3-acetic acid, and 0.46 μM kinetin at pH 5.5 was most effective for induction of shoot formation. Explants from the proximal end of the source lamina gave rise to a higher number of shoots compared to the mid and distal regions. Cultivar Tinora Red was more regenerative than Senator in terms of number of shoots per explant. The use of a lower BA concentration (0.44 μM) was essential for callus-free shoot multiplication during subculture. Regenerated shoots could be induced to form roots on half-strength MS medium supplemented with 0.54 μM α-naphthaleneacetic acid and 0.93 μM kinetin. More than 300 plantlets of each eultivar were harvested from a single source lamina within 200 d of culture. Most plantlets (95%) survived after acclimation in soil.
Key wordscultivars direct organogenesis meristemoids Tinora Red Senator
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- Atta-Alla, H.; McAlister, B.; van Staden, J. In vitro culture and establishment of Anthurium parvispathum. South African J. Bot. 64: 296–298; 1998.Google Scholar
- Carelli, B. P.; Echeverrigaray, S. An improved system for the in vitro propagation of rose cultivars. Sci. Hort. 91: 69–74; 2001.Google Scholar
- Eapen, S.; Rao, P. S. Regeneration of plant from Anthurium patulum. Curr. Sci. 54: 284–286; 1985.Google Scholar
- Kuehnle, A. R.; Sugii, N. Callus induction and plantlet regeneration of Hawaiian anthuriums. HortScience 26: 919–921; 1991.Google Scholar
- Liu, C. M.; Xu, Z. H. An efficient procedure for micropropagation of Anthurium scherzerianum Schott (flamingo flower). Chinese J. Bot. 4: 49–55; 1992.Google Scholar
- Matsumoto, T. K.; Kuehnle, A. R. Micropropagation of Anthurium. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry 40: high-tech and micropropagation VI. New York: Springer-Verlag; 1997: 15–29.Google Scholar
- Rajasekaran, K.; Hein, M. B.; Davic, G. C.; Carnes, M. G.; Vasil, I. K. Endogenous growth regulators in leaf cultures of Pennisetum purpureum Schum. J. Plant Physiol. 130: 13–25; 1987.Google Scholar
- Skirvin, R. M.; McPheeters, K. P.; Norton, M. A. Sources and frequency of somaclonal variation. HortScience 29: 1232–1237; 1994.Google Scholar
- Thorpe, T. A.; Harvy, I. S.; Kumar, P. P. Application of micropropagation in forestry. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation, technology and application. Dordrecht: Kluwer Academic Publishers; 1991: 311–336.Google Scholar
- Welander, M. Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J. Plant Physiol. 132: 738–744; 1988.Google Scholar