In Vitro Cellular & Developmental Biology - Plant

, Volume 39, Issue 5, pp 500–504 | Cite as

Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort.

  • K. P. Martin
  • Dominic Joseph
  • Joseph Madasser
  • V. J. Philip


Direct plant regeneration from flowering plant-derived lamina explants of Anthurium andraeanum Hort. cultivars Tinora Red and Senator was established on modified Murashige and Skoog (MS) medium. Cultivar difference, stage of source lamina and the position of explant in lamina, medium pH, and type of growth regulators significantly influenced direct plant regeneration. Explants from young brown lamina were superior to young green lamina. The half-strength MS medium containing 1.11 μM N6-benzyladenine (BA), 1.14 μM indole-3-acetic acid, and 0.46 μM kinetin at pH 5.5 was most effective for induction of shoot formation. Explants from the proximal end of the source lamina gave rise to a higher number of shoots compared to the mid and distal regions. Cultivar Tinora Red was more regenerative than Senator in terms of number of shoots per explant. The use of a lower BA concentration (0.44 μM) was essential for callus-free shoot multiplication during subculture. Regenerated shoots could be induced to form roots on half-strength MS medium supplemented with 0.54 μM α-naphthaleneacetic acid and 0.93 μM kinetin. More than 300 plantlets of each eultivar were harvested from a single source lamina within 200 d of culture. Most plantlets (95%) survived after acclimation in soil.

Key words

cultivars direct organogenesis meristemoids Tinora Red Senator 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Atta-Alla, H.; McAlister, B.; van Staden, J. In vitro culture and establishment of Anthurium parvispathum. South African J. Bot. 64: 296–298; 1998.Google Scholar
  2. Carelli, B. P.; Echeverrigaray, S. An improved system for the in vitro propagation of rose cultivars. Sci. Hort. 91: 69–74; 2001.Google Scholar
  3. Duncan, D. B. Multiple range and multiple F-tests. Biometrics 11: 1–42; 1955.CrossRefGoogle Scholar
  4. Eapen, S.; Rao, P. S. Regeneration of plant from Anthurium patulum. Curr. Sci. 54: 284–286; 1985.Google Scholar
  5. Joseph, D.; Martin, K. P.; Madassery, J.; Philip, V. J. In vitro propagation of three commercial cut flower cultivars of Anthurium andraeanum Hort. Indian J. Exp. Biol. 41: 154–159; 2003.PubMedGoogle Scholar
  6. Kuehnle, A. R.; Sugii, N. Callus induction and plantlet regeneration of Hawaiian anthuriums. HortScience 26: 919–921; 1991.Google Scholar
  7. Liu, C. M.; Xu, Z. H. An efficient procedure for micropropagation of Anthurium scherzerianum Schott (flamingo flower). Chinese J. Bot. 4: 49–55; 1992.Google Scholar
  8. Matsumoto, T. K.; Kuehnle, A. R. Micropropagation of Anthurium. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry 40: high-tech and micropropagation VI. New York: Springer-Verlag; 1997: 15–29.Google Scholar
  9. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays for tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.CrossRefGoogle Scholar
  10. Pierik, R. L. M. Anthurium andraeanum plantlets produced from callus tissues cultivated in vitro. Physiol. Plant. 37: 80–82; 1976.CrossRefGoogle Scholar
  11. Pierik, R. L. M.; Steegmans, H. H. M.; van der Meys, J. A. J. Plantlet formation in callus tissues of Anthurium andraeanum Lind. Sci. Hort. 2: 193–198; 1974.CrossRefGoogle Scholar
  12. Rajasekaran, K.; Hein, M. B.; Davic, G. C.; Carnes, M. G.; Vasil, I. K. Endogenous growth regulators in leaf cultures of Pennisetum purpureum Schum. J. Plant Physiol. 130: 13–25; 1987.Google Scholar
  13. Singh, S. K.; Syamal, M. M. A short pre-culture soak in thidiazuron or forchlorfenuron improves axillary shoot proliferation in rose micropropagation. Sci. Hort. 91: 169–177; 2001.CrossRefGoogle Scholar
  14. Skirvin, R. M.; McPheeters, K. P.; Norton, M. A. Sources and frequency of somaclonal variation. HortScience 29: 1232–1237; 1994.Google Scholar
  15. Teng, W.-L. Regeneration of Anthurium adventitious shoots using liquid or raft culture. Plant Cell Tiss. Organ Cult. 49: 153–156; 1997.CrossRefGoogle Scholar
  16. Thorpe, T. A.; Harvy, I. S.; Kumar, P. P. Application of micropropagation in forestry. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation, technology and application. Dordrecht: Kluwer Academic Publishers; 1991: 311–336.Google Scholar
  17. Welander, M. Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J. Plant Physiol. 132: 738–744; 1988.Google Scholar

Copyright information

© Society for In Vitro Biology 2003

Authors and Affiliations

  • K. P. Martin
    • 1
  • Dominic Joseph
    • 1
  • Joseph Madasser
    • 1
  • V. J. Philip
    • 1
  1. 1.Department of BiotechnologyUniversity of CalicutKeralaIndia

Personalised recommendations