Advertisement

In Vitro Cellular & Developmental Biology - Plant

, Volume 39, Issue 6, pp 618–622 | Cite as

Axillary bud development of passionfruit as affected by ethylene precursor and inhibitors

  • L. B. Reis
  • V. B. Paiva Neto
  • E. A. Toledo Picoli
  • M. G. C. Costa
  • M. M. Rêgo
  • C. R. Carvalho
  • F. L. Finger
  • W. C. Otoni
Article

Summary

The effects of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and two inhibitors, silver thiosulfate (STS) and aminoethoxyvinylglycine (AVG), were tested in yellow passionfruit (Passiflora edulis f. flaricarpa Degener) acillary cultured in vitro. The organogenic responses were assessed by the number of buds per explant, mean leaf area per explant, and shoot length. ACC-supplemented medium significantly inhibited all evaluated responses at both concentrations tested. When ethylene action and biosynthesis were inhibited, a significant increase in the number of developed buds and average leaf area was observed. Accumulated ethylene and its accumulation rate were significantly greater at 10 μM ACC, with a maximum production rate deteeted: at the 14th day and a decline at the 21st day. The results suggest beneficial effects of ethylene inhibitors on in vitro development of axillary buds and their reliability for use as an alternative approach to evaluate sensitivity of Passiflora species to ethylene. Even though shoot elongation did not differ from that of the control, the inhibition of the ethylene action and its biosynthesis by AVG and STS, respectively, significantly enhanced the number of buds per explant and leaf area.

Key words

plant tissue culture ethylene Passiflora edulis f. flavicarpa Degener shoot culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arjona, H. E.; Matta, F. B. Postharvest quality of passionfruit as influenced by harvest time and ethylene treatment. Hort Science 26:1297–1298; 1991.Google Scholar
  2. Barbosa, W. M.; Otoni, W. C.; Canelossi, M.; Silva, E.; Azevedo, A. A.; Vieira, C. Rinzogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursors and by inhibitors. Int. J. Hort. Sci. 7:47–51; 2001.Google Scholar
  3. Biondi, S.; Diaz, T.; Iglesias, I.; Gamberini, G.; Bagni, N. Polyamines and ethylene in relation to adventitious root formation in Prunus arium shoot cultures. Physiol. Plant. 78:474–483; 1990.CrossRefGoogle Scholar
  4. Biondi, S.; Scaramagli, S.; Capitani, F.; Marino, G.; Altamura, M. M.; Torrigiani, P. Ethylene involvement in vegetative bund formation in tobacco thin layers. Protoplasma 202:134–144; 1998.CrossRefGoogle Scholar
  5. Chi, G.-L.; Barfield, D. G.; Sim, G.-E.; Pua, E.-C. Effect of AgNO3 and aminoethoxyvinyglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep. 9:195–198; 1990.CrossRefGoogle Scholar
  6. Chi, G.-L.; Pua, E.-C.; Goh, C. J. Role of ethylene on de novo shoot regeneration from cotyledons of Brassica campestris ssp, pekinensis (Lour) Olsson in vitro. Plant Physiol. 96:178–183; 1991.PubMedGoogle Scholar
  7. Drew, R. In vitro culture of adult and juvenile bud explants of Passiflora species. Plant Cell Tiss. Organ Cult. 26:23–27; 1991.CrossRefGoogle Scholar
  8. Faria, F. L. C.; Segura, J. In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis f. flaricarpa. In Vitro Cell. Dev. Biol. Plant 33:209–212; 1997.Google Scholar
  9. Gamborg, O. L.; Miller, R. A.; Ojima, K. Numert requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.PubMedCrossRefGoogle Scholar
  10. George, E. F. Plant propagation by tissue culture. Part 1:The technology, 2nd edn. Edington, Wilts: Exegetics Ltd.: 1993.Google Scholar
  11. Goh, C. J.; Ng, S. K. Lakshmanan, P.; Loh, C. S. The role ethylene on direct shood but regeneration from mangosteen (Garcinia mangostana L.) leaves cultured in vitro. Plant Sci. 124:193–202; 1997.CrossRefGoogle Scholar
  12. Huxter, T. J.; Thorpe, T. A.; Reid, D. M. Shoot initiation in light-and darkgown tobacco callus: the role of ethylene. Physiol. Plant. 53:319–326; 1981.CrossRefGoogle Scholar
  13. Kantharajah, A. S.; Dodd, W. A. In nitro micropropagation of Passiflora edulis (purple passion fruit). Ann. Bot. 65:337–339; 1990.Google Scholar
  14. Kawata, K.; Ushida, C.; Kawai, F.; Kanamori, M.; Kuriyama, A. Micropropagation of passion fruit subcultured multiple shoot primordia. J. Plant Physiol. 147:281–284; 1995.Google Scholar
  15. Kumar, P. P.; Lakshmanan, P.; Thorpe, T. A. Regulation of morphogenesis in plant tissue culture by ethylene. In Vitro Cell. Dev. Biol. Plant 34:94–103; 1998.CrossRefGoogle Scholar
  16. Ma, J. H.; Yao, J. L.; Cohen, D.; Morris, B. Ethylene inhibitors enhance in vitro root formation from apple shoot cultures. Plant Cell Rep. 17:211–214; 1998.CrossRefGoogle Scholar
  17. Magdalita, P. M.; Godwin, I. D.; Drew, R. A.; Adkins, S. W. Effect of ethylene and culture environment on development of papaya nodal cultures. Plant Cell Tiss. Organ Cult. 49:93–100; 1997.CrossRefGoogle Scholar
  18. Matthijs, D.; Gielis, J.; Debergh, P. Ethylene. In: Aitken-Christie, J.; Kozai, T.; Smith, M. A. L., eds. Automation and environmental control in plant tissue culture. Dordrecht: Kluwer Academic Publishers; 1995:473–491.Google Scholar
  19. Meletti, L.; Maia, M. L. Maracujá: produção e comercialização. Campinas: Institito Agronômico; 1999.Google Scholar
  20. Monteiro, A. C. B.; Higashi, E. N.; Gonçalves, A. N.; Rodriguez, A. P. M. A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis Sims. F. flavicarpa Deg). In Vitro Cell. Dev. Biol. Plant 36:527–531; 2000.Google Scholar
  21. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.CrossRefGoogle Scholar
  22. Nissen, P. Stinrulation of somatic embryogenesis in carrot by ethylene. In: Pech, J. C.; Latché, A.; Balgué, C., eds. Cellular and molecular aspects of the plant hormone ethylene. Dordrecht: Kluwer Academic Publishers; 1993:359–364Google Scholar
  23. Pocasangre-Enamorado, H. E.; Finger, F. L.; Barros, R.S.; Puschmann, R. Development and ripening of yellow passion fruit. J. Hort. Sci. 70:573–576; 1995.Google Scholar
  24. Pua, E.-C. Morphogencsis in cell and tissue cultures: role of ethylene and polyamines. In: Soh, W.-Y.; Bhojwani, S. S., eds Morphogenesis in plant tissue cultures. London: Springer-Verlag; 1999:255–303.Google Scholar
  25. Pua, E.-C.; Sim, G.-E.; Chi, C.-L.; Kong, L.-F. Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus) in vitro. Plant Cell Rep. 15:685–690; 1996.CrossRefGoogle Scholar
  26. Purnhauser, L.; Medgyesy, P.; Dix, P. J.; Márton, L Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3. Plant Cell Rep. 6:1–4; 1987.CrossRefGoogle Scholar
  27. Reid, M. S.; Paul, J. L.; Farhoomand, M. B.; Kofranek, A. M.; Staby, G. L. Pulse treatments with the silver thiosulfate complex extend the vase life of cut carnations. J. Am. Soc. Hort. Sci. 105:25–27; 1980.Google Scholar
  28. Reis, L. B. Morfogênese in vitro de maracujá-amarclo (Passiflora edulis f. flavicarpa Degener) associada ao etileno e a agentes gelificantes. Masters thesis, Universidade Federal de Viçosa. Viçosa; 2001 (in Portuguese).Google Scholar
  29. Sankhla, D.; Sankhla, N.; Davis, D Promotion of in vitro shoot formation from excised roots of silktree (Albizzia julibrissin) by an oxime ether derivative and other ethylene inhibitors. Plant Cell Rep. 15:143–146; 1995.CrossRefGoogle Scholar
  30. Santos, K. G. B.; Mundstock, E.; Bodanese-Zanettini, M. H. Genotypespecific normalization of soybean somatic embryogenesis through the use of an ethylene inhibitor. Plant Cell Rep. 16:859–864; 1997.CrossRefGoogle Scholar
  31. Shiomi, S.; Kubo, Y.; Wamocho, L. S.; Koaze, H.; Nakamura, R; Inaba, A. Postharyest ripening and ethylene biosynthesis in purple passion fruit. Postharvest Biol. Technol. 8:199–207; 1996.CrossRefGoogle Scholar
  32. Taylor, J. E. Exotics. In: Seymour, G. B.; Taylor, J. E.; Tucker, J. A., eds. Biochemistry of fruit ripening. London: Chapman & Hall; 1993:151–187.Google Scholar

Copyright information

© Society for In Vitro Biology 2003

Authors and Affiliations

  • L. B. Reis
    • 1
  • V. B. Paiva Neto
    • 1
  • E. A. Toledo Picoli
    • 1
  • M. G. C. Costa
    • 1
  • M. M. Rêgo
    • 2
  • C. R. Carvalho
    • 3
  • F. L. Finger
    • 2
  • W. C. Otoni
    • 1
  1. 1.Department of Biologia VegetalUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Departamento de FitotecniaUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Departmento Biologia GeralUniversidade Federal de ViçosaViçosaBrazil

Personalised recommendations