Skip to main content
Log in

In vitro propagation of Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann (cactaceae)

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

Development of efficient in vitro propagation, systems for Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann, two endangered Mexican species of cacti, are described. Multiple shoot formation from areoles of in vitro-germinated plantlets was achived in two types of explants (apical and transversal) cultured in Murashige and Skoog (MS) basal media supplemented with 30 or 50 gl−1 sucrose, 10 gl−1 agar and various treatments with cytokins. Shoot production in these proliferation media was evaluated after one (60 d) and three (180 d) culture cycles. In P. asellifornis 13.7 shoots per explant were produced after the first cycle using apical explants in medium with 8.8 μM 6-benzylaminopurine (BA) and 30 gl−1 sucrose. In P. strobiliformis the highest proliferation rate (12.4 shoots per explant) was reached using 8.8 μM Ba and 50 gl−1 sucrose with shoot transverse segments as explants. After the third proliferation cycle, 128.1 and 136.3 shoots per explant were obtained in P. aselliformis and P. strobiliformis, respectively. The shoots were clongated in MS basal medium with 3 gl−1 activated charcoal and rooted in MS basal media indoleacetic acid (2.85 or 5.71 μM) or indolebutyric acid (2.46 or 4.90 μM). On averge, rooting efficiency was 89% for P. aselliformis and 87% for P. strobiliformis. The survival frequency, of the plants once transferred, to soil was on average 88%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ault, J. R.; Blackmon, W. J. In vitro propagation of Ferocactus acanthodes (Cactaceae) HortSci. 22:126–127, 1987.

    Google Scholar 

  • Elias-Rocha, M. A., Sántos-Díaz, M. S.; Arredondo-Gómez, A. Propagation of Mammillaria, candida (Cactaceae) by tissue culture techniques. Haseltonia 6:96–101; 1998.

    Google Scholar 

  • Escobar, H. A.; Villalobos, A. V. M.; Villegas, M. A. Opuntia micropropagation by axillary proliferation. Plant Cell Tiss. Organ Cult. 7:269–277; 1986.

    Article  Google Scholar 

  • George, E. F. Plant propagation by tissue culture. Part 2. In practice, 2nd edn. London: Exgetics Ltd.; 1996:903–908.

    Google Scholar 

  • Glass, C. Guide to the identification of threated cacti of México México: Ediciones Cante: 1998:141–144.

    Google Scholar 

  • Hemphill, J. K.; Maier, C. G. A., Chapman, K. D. Rapid in-vitro plant regeneration of cotton (Gossypium hirsutum L.). Plant Cell Rep. 17:273–278:1998.

    Article  CAS  Google Scholar 

  • Hubstenberger, J. F.; Clayton, P. W.; Phillips, G. C. Micropropagation of cacti (Cactaceae). In: Bajaj, Y. P. S., ed Biotechnology in agriculture and forestry, vol. 20. High-tech, and micropropagation IV Berlin: Springer-Verlag; 1992:49–68.

    Google Scholar 

  • Infate, R. In vitro axillary shoot proliferation and somatic embryogenesis of yellow, pitaya Mediocatus coccineus (Salm-Dyck). Plant Cell Tiss. Organ Cult. 31:155–159; 1992.

    Article  Google Scholar 

  • Machado, M. F. P. S.; Prioli, A. J. Micropropagation of Cereux peruvianus Mill. (Cactaceae) by aeole activation. In Vitro Cell. Dev. Biol. Plant 32:199–203; 1996.

    Google Scholar 

  • Malda, G.; Backhaus R. A.; Martin, C.: Alterations in growth and crassulancean acid metabolism (CAM) activity of in vitro cultured cactus. Plant Cell Tiss. Organ Cult. 58:1–9; 1999a.

    Article  CAS  Google Scholar 

  • Malda, G.; Suzan, H.; Backhaus, R. In vitro cultures as a potential method for the conservation of endangenered plants processing crassulacean acid metabolism. Sci. Hortic. 81:71–87; 1999b.

    Article  CAS  Google Scholar 

  • Martínez-Vázquez, O.; Rubluo, A. In vitro mass propagation of the near-extinct Mammillaria san-angelensis Sánchez-Mejorada J. Hort. Sci. 64:99–105:1989.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Oliveira, S. A.; Machado, M. F. P. S.; Prioli, A. J. In vitro propagation of Cereus peruvianus Mill (Cactacea). In Vitro Cell. Dev. Biol. Plant 31:47–50; 1995.

    Google Scholar 

  • Pérez-Molphe-Balch, E.; Pérez-Reyes, M. E.; Villalobos-Amador, E.; Meza-Rangel, E.; Morones Ruiz, L. R.; Lizadle-Viramontes, H. Micropropagation of 21 species of Mexican Cacti by axillary proliferation. In Vitro Cell. Dev. Biol. Plant 34:131–135; 1998.

    Google Scholar 

  • Rodriguez-Garay, B.; Rubluo, A. In vitro morphogenetic responses of the endangered cactus Aztekium ritteri (Boedeker). Cact. Succ. J. (US) 64:116–119; 1992.

    Google Scholar 

  • Ziv, M., Vitrification: morphological and physiological disorders of in vitro plants. In: debergh, P. C., Zimmerman, R. H., eds. Micropropagation. Technology and application. Dordrecht: Kluwer Academic Publishers, 1991:45–69.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Eugenio Pérez-Molphe-Balch.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez-Molphe-Balch, E., Dávila-Figueroa, C.A. In vitro propagation of Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann (cactaceae). In Vitro Cell.Dev.Biol.-Plant 38, 73–78 (2002). https://doi.org/10.1079/IVP2001248

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1079/IVP2001248

Key words

Navigation