International Journal of Tropical Insect Science

, Volume 24, Issue 3, pp 228–235 | Cite as

Potential leafhopper vectors of phytoplasma in carrots

  • Phyllis G. WeintraubEmail author
  • Sigalit Orenstein


Phytoplasmas are insect-vectored pathogens that cause characteristic and destructive diseases in carrots and other vegetables. A phytoplasma disease was first observed in Israeli carrot fields in 1995. Analysis of infected carrots showed the presence of aster yellows and western-X phytoplasmas. In this study, commercial and experimental fields in the western Negev region of Israel were monitored for three years using yellow sticky traps and vacuum sampling. Potential vectors of leafhoppers and planthoppers were analysed by PCR for the presence of phytoplasma DNA. Infected plants were also assayed for phytoplasma DNA. Extracted phytoplasma DNA was subjected to RFLP analysis to determine groups to which the phytoplasmas belonged. It was determined that carrots and leafhoppers from the experimental station were infected with a phytoplasma belonging to the Elm Yellows (EY) group; this is the first report of EY infecting carrots. Based on our findings, the two most probable insect vectors are Circulifer haematoceps complex (Mulsant & Rey) and Neoaliturus fenestratus (Herrich-Schäffer).

Key words

carrot phytoplasma vectors Circulifer haematoceps Neoaliturus fenestratus 

Mots clés

carotte phytoplasma vecteurs Circulifer haematoceps Neoaliturus fenestratus 


Les phytoplasmas sont des pathogènes transmis par des insectes vecteurs, responsables de maladies destructives et caractéristiques sur les carottes et d’autres légumes. Une maladie à phytoplasma a été observée pour la première fois en Israël en 1995 dans des champs de carottes. L’analyse des carottes infectées à révélé la présence des phytoplasmas aster jaunes et western-X. Dans cette étude, des parcelles expérimentales et commerciales situées dans la région Ouest du Negev en Israël ont été suivis pendant 3 ans en utilisant des pièges englués jaunes et des pièges à succion. Les vecteurs potentiels, des cicadelles et les jassides, ont été analysés par PCR afin de contrôler la présence du DNA de phytoplasma. Les plantes infectées ont également été contrôlées pour le DNA de phytoplasma. Le DNA extrait a été soumis à une analyse par RFLP afin de déterminer le groupe d’appartenance des phytoplasmas. On a ainsi pu déterminer que les carottes et les jassides de la station expérimentale étaient infectés par des phytoplasmas du groupe Elm Yellows (EY); Il s’agit du premier signalement de carottes infectées par des phytoplasmas du groupe EY. Sur la base de nos résultats, le complexe Circulifer haematoceps (Mulsant et Rey) et Neoaliturus fenestratus (Herrich-Schäffer) sont probablement les deux principaux vecteurs.


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  1. Beanland L., Hoy C. W., Miller S. A. and Nault L. R. (1999) Leafhopper (Homoptera: Cicadellidae) transmission of aster yellows phytoplasma: does gender matter? Environ. Entomol. 28, 1101–1106.CrossRefGoogle Scholar
  2. Bedendo I. P., Davis R. E. and Dally E. L. (1999) Detection and identification of phytoplasmas in periwinkle (Catharanthus roseus) and pepper (Capsicum frutescens) by nested PCR and RFLP techniques. Summa Phytopathol. 25, 197–201.Google Scholar
  3. Burkness E. C., Venette R. C., O’ Rourke P. K. and Hutchison W. D. (1999) Bionomial sequential sampling for management of aster leafhopper (Homoptera: Cicadellidae) and aster yellows phytoplasma in carrot: impact of tally threshold on the accuracy of treatment decisions. Environ. Entomol. 28, 851–857.CrossRefGoogle Scholar
  4. Chiykowski L. N. (1981) Epidemiology of diseases caused by leafhopper-borne pathogens, pp. 106–159. In Plant Disease and Vectors (Edited by K. Maramorosch and K. F. Harris). Academic Press, New York.Google Scholar
  5. Fránová J., Karesová R., Navrátil M., Simková M., Válová P. and Nebesárová J. (2000) A carrot proliferation disease associated with rickettsia-like organisms in the Czech Republic. J. Phytopathol. 148, 53–55.CrossRefGoogle Scholar
  6. Gabelman W. H., Goldman I. L. and Breitbach D. N. (1994) Field evaluation and selection for resistance to aster yellows in carrot (Daucus carota L.). J. Amer. Soc. Hort. Sci. 119, 1293–1297.CrossRefGoogle Scholar
  7. Giannotti J., Louis C., Leclant F., Marchoux G. and Vago C. (1974) Infection a mycoplasmes et a micro-organismes d’allure rickettsienne chez une plante atteinte de proliferation et chez le pshylle vecteur de la maladie. CR. Acad. Sci. Paris Ser. D 278, 469–471.Google Scholar
  8. Gibb, K., Tran-Nguyen, L. and Davison, E. M. (2001) What causes purple-tops and hairy roots in western Australian carrots? Proceedings of the Thirteenth Biennial Conference of the Australasian Plant Pathology Society, 24–27 Sept 2001, Cairns, Queensland.Google Scholar
  9. Goodwin P. H., Mahuku G. S., Liu H. and Xue B. G. (1999) Monitoring phytoplasma in populations of aster leafhoppers from lettuce fields using the polymerase chain reaction. Crop Protection 18, 91–99.CrossRefGoogle Scholar
  10. Hoy C. W., Heady S. E. and Koch T. A. (1992) Species composition, phenology, and possible origins of leafhoppers (Cicadellidae) in Ohio vegetable crops. J. Econ. Entomol. 85, 2336–2343.CrossRefGoogle Scholar
  11. Hunt R. E., Parr J. C. and Haynes K. F. (1993) Influence of leafhopper (Homoptera: Cicadellidae) gender and female mating status on plant disease dynamics within a simple habitat. Environ. Entomol. 22, 109–115.CrossRefGoogle Scholar
  12. Irwin M. E. and Ruesink W. G. (1986) Vector intensity: a product of propensity and activity, pp. 13–33. In Plant Virus Epidemics: Monitoring, Modeling and Predicting Outbreaks (Edited by G. D. McLean, R. G. Garrett and W. G. Ruesink). Academic Press, New York.Google Scholar
  13. Ivanoff S. S. and Ewart W. H. (1944) Aster yellows on vegetable crops and weeds in winter garden region of Texas. Plant Dis. Rep. 28, 972–977.Google Scholar
  14. Klein M., Weintraub P. G., Davidovich M., Kuznetsova L., Zahavi T., Ashanova A., Orenstein S. and Tanne E. (2001) Monitoring phytoplasma-bearing leafhoppers/planthoppers in vineyards in the Golan Heights. Israel J. Appl. Entomol. 123, 19–23.CrossRefGoogle Scholar
  15. Lorenz K. H., Schneider B., Ahrens U. and Seemuller E. (1995) Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology 85, 771–776.CrossRefGoogle Scholar
  16. Maixner M., Ahrens U. and Seemuller E. (1995) Detection of German grapevine yellows (Vergilbungs Krankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. Eur. J. Plant Pathol. 101, 241–250.CrossRefGoogle Scholar
  17. Marzachi C., Alma A., d’ Aquilio N., Minuto G. and Boccardo G. (1999) Detection and identification of phytoplasmas infecting cultivated and wild plants in Liguria (Italian Riviera). J. Plant Pathol. 81, 127–136.Google Scholar
  18. Orenstein S., Franck A., Kuznetzova L., Sela I. and Tanne E. (1999) Association of phytoplasmas with a yellows disease of carrot in Israel. J. Plant Pathol. 81, 193–199.Google Scholar
  19. Orenstein S., Zahavi T. and Weintraub P. G. (2001) Survey of the distribution of phytoplasma in wine grapes in the Golan Heights, Israel and development of specific primers. Vitis 40, 219–223.Google Scholar
  20. Orenstein S., Zahavi T., Nestel D., Sharon R., Barkalifa M. and Weintraub P. G. (2003) Spatial dispersion patterns of potential leafhopper and planthopper (Homoptera) vectors of phytoplasma in wine vineyards. Ann. Appl. Biol. 143.Google Scholar
  21. Power A. G. (1987) Plant community diversity, herbivore movement, and an insect-transmitted disease of maize. Ecology 68, 1658–1669.CrossRefGoogle Scholar
  22. Power A. G. (1992) Host plant dispersion, leafhopper movement and disease transmission. Ecol. Entomol. 17, 63–68.CrossRefGoogle Scholar
  23. Purcell A. H., Richardson J. and Finlay A. (1981) Multiplication of the agent of X-disease in a non-vector leafhopper Macrosteles fascifrons. Ann. Appl. Biol. 99, 283–289.CrossRefGoogle Scholar
  24. Severin H. H. P. (1934) Transmission of California aster and celery-yellows virus by three species of leafhoppers. Hilgardia 8, 339–362.Google Scholar
  25. Severin H. H. P. and Frazier N. W. (1945) California aster yellows on vegetable and seed crops. Hilgardia 16, 573–596.CrossRefGoogle Scholar
  26. Vega F. E., Davis R. E., Barbose P., Dally E. L., Purcell A. H. and Lee I.-M. (1993) Detection of a plant pathogen in a nonvector insect species by the polymerase chain reaction. Phytopathology 83, 621–624.CrossRefGoogle Scholar

Copyright information

© ICIPE 2004

Authors and Affiliations

  1. 1.Department of Entomology, Agricultural Research Organization (ARO)Gilat Research CenterD.N. NegevIsrael
  2. 2.Northern Research and DevelopmentKiryat Sh’monaIsrael

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