Abstract
The effectiveness of three media, peptone-PCNB agar (PPA), dichloran-chloramphenicol agar (DCPA) and modified potato-dextrose agar (MPDA) for the recovery of Fusarium, Alternaria and other fungi from sorghum grain was evaluated using three grain samples. One sample appeared free of mould damage on visual inspection whereas the other two samples had obvious mould damage and were discoloured. PPA was the most effective medium for the recovery of Fusarium spp. while DCPA was most effective in recovering Alternaria spp. MPDA proved useful for the recovery of Phoma sorghina and a number of dematiaceous fungal species. A. alternata was frequently recovered from the clean sorghum sample but rarely recovered from the two mouldy samples. F. moniliforme, F. equiseti, F. chlamydosporum and F. semitectum were all isolated at relatively high frequencies from the two mouldy sorghum samples but only F. moniliforme was recovered from the clean sample. P. sorghina was recovered at high frequencies from the two mouldy samples but not from the clean sample. Thirty species of fungi, including 13 species of Fusarium, were isolated from the two mouldy sorghum samples.
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Ali, H., Summerell, B.A. & Burgess, L.W. An evaluation of three media for the isolation of Fusarium, Alternaria and other fungi from sorghum grain. Australasian Plant Pathology 20, 134–138 (1991). https://doi.org/10.1071/APP9910134
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DOI: https://doi.org/10.1071/APP9910134