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Cryopreservation of air-dried conidia of Uncinula necator

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Abstract

Conidia of Uncinula necator, the causal agent of grapevine powdery mildew, were viable after storage at −70°C for up to 12 months following air drying without cryoprotectants, as determined by ability to infect surface-sterilised, detached grapevine leaves. The fungus was stored as cyclone-harvested conidia and on segments of infected leaves. Conidia also survived five freezelthaw cycles. Southern blot analysis revealed that DNA extracted from colonies resulting from cryopreserved and fresh conidia produced the same DNA fingerprints.

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Authors and Affiliations

  1. Cooperative Research Centre for Viticulture, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, South Australia, Australia

    B. E. Stummel, T. Zanke & E. S. Scott

  2. Department of Crop Protection, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, South Australia, Australia

    B. E. Stummel, T. Zanke & E. S. Scott

Authors
  1. B. E. Stummel
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  2. T. Zanke
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  3. E. S. Scott
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Correspondence to E. S. Scott.

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Stummel, B.E., Zanke, T. & Scott, E.S. Cryopreservation of air-dried conidia of Uncinula necator . Australasian Plant Pathology 28, 82–84 (1999). https://doi.org/10.1071/AP99012

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  • DOI: https://doi.org/10.1071/AP99012

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