Abstract
The orchid Sarcochilus hartmanii × Sarcochilus falcatus showing flower virescence with some evidence of stunting was found during an orchid virus survey of south-east Queensland. Phytoplasma bodies were detected by thinsection electron microscopy and phytoplasma DNA was detected using the polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) analysis was used to compare this phytoplasma with those found in dicotyledonous plants. RFLP analysis demonstrated that this orchid phytoplasma was indistinguishable from the tomato big bud phytoplasma.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahrens, U. and Seemüller, E. (1992) — Detection of plant pathogenic mycoplasma-like organisms by polymerase chain reaction that amplified a sequence of the 16S rRNA gene. Phytopathology 82: 828–832.
Bowyer, J.W. (1974) — Tomato Big Bud, Legume Little Leaf, and Lucerne Witches’ Broom: Three diseases associated with different mycoplasma-like organisms in Australia. Australian Journal of Agricultural Research 25: 449–457.
Bowyer, J.W. and Atherton, J.G. (1971) — Mycoplasmalike bodies in french bean, dodder, and the leafhopper vector of the little leaf agent. Australian Journal of Biological Sciences 24: 717–729.
Davis, R.I., Schneider, B. and Gibb, K.S. (1997) — Detection and differentiation of phytoplasmas in Australia. Australian Journal of Agricultural Research 48: 535–544.
Deng, S. and Hiruki, C. (1991) — Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiological Methods 14: 53–61,
Hopkins, D.L. (1977) — Diseases caused by leafhopper-borne, rickettsia-like bacteria. Annual Review of Phytopathology 15: 277–294.
Kirkpatrick, B.C., Smart, C.D., Gardener, S.L., Gao, J.L., Ahrens, U., Mäurer, R., Schneider, B., Lorenz, K-H., Seemüller, E., Hamson, NA., Namba, S. and Daire, X. (1994) — Phylogenetic relationships of plant pathogenic MLOs established by 16/238 rDNA spacer sequences. International Organisation for Mycoplasmologv Letters 3: 228–229.
Marwitz, R. and Petzold, H. (1972) — Nachweis mykoplasmaahnlicher organismen. Phytopathologische Zeitschrift 75: 362–364
McCoy, R.E., Caudwell, A., Chang, C.J., Chen, T.A., Chiykowski, L.N., Cousin, M.T., Dale, J.L., deLeeuw, G.T.N., Golino, D.A., Hackett, K.J., Kirkpatrick, B.C., Manvitz, R., Petzold, H., Sinha, R.C., Sugiura, M., Whitcomb, R.F., Yang, I.L., Zhu, B.M., and Seemtiller, E. (1989) — Plant disease associated with mycoplasma-like organisms. In The Mycoplasmas Volume Spiroplasmas, Acheloplasmas, and Mycoplasmas of Pants and Arthropods (Eds R.F. Whitcomb and J.G. Tully), pp. 545–640. Academic Press, New York.
Pegg, K.G. and Stirling, G.R. (1994) — Ginger diseases In: Diseases of Vegetable Crops (Ed D.M. Persley), Department of Primary Industries, Queensland.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gowanlock, D.H., Ogle, H.J. & Gibb, K.S. Phytoplasma associated with virescence in an epiphytic orchid in Australia. Australasian Plant Pathology 27, 265–268 (1998). https://doi.org/10.1071/AP98031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1071/AP98031