Abstract
A reverse transcription polymerase chain reaction system was developed to detect passionfruit woodiness potyvirus (PWV) in leaves of Passiflora species. PWV coat protein (CP) sequences which were amplified from New South Wales isolates and PWV strain K had identical Hinf I, Hae III, Rsa I and Cfo I restriction profiles. Some polymorphism was revealed by digestion with Alu I. PWV CP sequences from 20 out of 21 plants had identical Alu I patterns which differed from that of PWV-K. The remaining isolate produced Ah I restriction fragments characteristic of both the common New South Wales isolates and of PWV-K, suggesting that the original plant contained a mixed infection. Three CP cDNA clones, each from an individual plant sample, and six clones from the sample which showed a mixed infection were sequenced. Analysis of the sequence data showed that these isolates from New South Wales had over 97% nucleotide sequence similarity and were closely related to PWV-K. There was 82–85% similarity in the deduced amino acid sequence to the previously described tip blight, severe and mild strains.
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Allison, R.F., Dougherty, R.G., Parks, T.D., Willis, J., Johnston, R.E., Kelly, M.E. and Armstrong, F.B. (1985) — Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids located on the virion’s surface. Virology 147: 309–316.
Chou, Q., Russell, M., David, E.B., Jonathan, R.F. and Bloch, W. (1992) — Prevention of pre-PCR mispriming and primer dimerization improves lowcopy-number amplifications. Nucleic Acids Research 20:1717–1723.
Davies, C. and Symons, R.H. (1988) — Further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus RNA 3. Virology 165: 216–224.
Dodds, J.A., Jarupat, T., Lee, J.G. and Roistacher, C.N. (1987) — Effects of strain, host, time of harvest, and virus concentration on double-stranded RNA analysis of citrus tristeza virus. Phytopathology 77: 442–447.
Echols, H. and Goodman, M.F. (1991) — Fidelity mechanisms in DNA replication. Annual Review of Biochemistry 60: 477–511.
Felsenstein, J. (1993) — PHYLIP (Phylogeny Inference Package) version 3.57c. istributed by the author. Department of Genetics, University of Washington, Seattle.
Feng, D. F. and Doolittle, R.F. (1987) — Progressive sequence alignment as a prerequisite to correct phylogenetic trees. Journal of Molecular Evolution 25: 351–360.
Gillings, M., Broadbent, P., Indsto, J. and Lee, R. (1993) — Characterization of isolates and strains of citrus tristeza closterovirus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction. Journal of Virological Methods 44: 305–317.
Gough, K.H. and Shukla, D.D. (1992) — Major sequence variations in the N-terminal region of the capsid protein of a severe strain of passionfruit woodiness potyvirus. Archives of Virology 124: 389–396.
Greber, R.S. (1966) — Passionfruit woodiness virus as the cause of passion vine tip blight disease. Queensland Journal of Agricultural and Animal Sciences 23: 533–538.
Henson, J.M. and French, R. (1993) — The polymerase chain reaction and plant disease diagnosis. Annual Review of Phytopathology 31: 81–109.
Hollings, M. and Brunt, A.A. (1981) — Potyviruses. In Handbook of Plant Krus Infections: Comparative Diagnosis. (Ed E. Kurstak), pp. 731–807. Elsevier/North Holland, New York.
Lomonossoff, G.P. (1995) — Pathogen-derived resistance to plant viruses. Annual Review of Phytopathology 33: 323–343.
Matthews, R.E.F. (1985) — Viral taxonomy for the nonvirologist. Annual Review of Microbiology 39: 451–474.
Pares, R.D., Gillings, M.R. and Gunn, L.V. (1992) — Differentiation of biologically distinct cucumber mosaic virus isolates by PAGE of double-stranded R.NA. Intervirology 34: 24–39.
Pares, R.D., Gunn, L.V., Keskula, E.N., Martin, A.B. and Teakle, D.S. (1997) — Occurrence of Passiflora latent carlavirus in cultivated and wild Passiflora species in Australia. Plant Disease 81: 348–350.
Pares, R.D., Martin, A.B. and Fitzell, R.D. (1985) — Virus-induced tip necrosis of passionfruit (Passiflora edulis Sims). Australasian Plant Pathology 14:76–78.
Pares, R.D., Martin, A.B. and Morrison, W. (1983) — Rhabdovirus-like particles in passionfruit. Australasian Plant Pathology 12:51–52.
Peasley, D. and Fitzell, R.D. (1981) — Passionfruit industry benefits through scionwood scheme. Agricultural Gazette of New South Wales 92: 5–8.
Riechmann, J.L., Lain, S. and Garcia, J.A. (1992) — Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73: 1–16.
Rizos, H., Gunn, L.V., Pares, R.D. and Gillings, M.R. (1992) — Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology 73: 2099–2103.
Saitou, N. and Nei, M. (1987) — The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) — Molecular Cloning, A Laboratory Manual, 2nd edn. New York: Cold Spring Harbour Laboratory.
Saris, P.E.J., Paulin, L.G. and Uhlén, M. (1990) — Direct amplification of DNA from colonies of Bacillus subtilis and Escherichia coli by the polymerase chain reaction. Journal of Microbiological Methods 11: 121–126.
Shukla, D.D., McKern, N.M. and Ward, C.W. (1988) — Coat protein of potyviruses 5: symptomatology, serology, and coat protein sequence of three strains of passionfruit woodiness virus. Archives of Virology 102: 221–231.
Shukla, D.D. and Ward, C.W. (1989) — Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106: 171–200.
Shukla, D.D., Ward, C.W. and Brunt, A.A. (1994) — The Potyviridae. CAB International, Cambridge.
Skotnicki, M.L., Mackenzie, A.M. and Gibbs, A.J. (1996) — Genetic variation of turnip yellow mosaic tymovirus within single plants of Cardamine lilacina. Australasian Plant Pathology 25: 249–254.
Stark, D.M. and Beachy, R.N. (1989) — Protection against potyvinis infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262.
Valverde, R.A., Nameth, S.T. and Jordan, R.L. (1990) — Analysis of double-stranded RNA for plant virus diagnosis. Plant Disease 74: 255–258.
Van Regenmortel, M.H.V. (1982) — Serology and Immunochemistry of Plant Viruses. Academic Press, New York.
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Sokhandan, N., Gillings, M.R. & Bowyer, J.W. Polymerase chain reaction detection and assessment of genetic variation in New South Wales isolates of passionfruit woodiness potyvirus. Australasian Plant Pathology 26, 155–164 (1997). https://doi.org/10.1071/AP97026
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DOI: https://doi.org/10.1071/AP97026