Abstract
Transgenic tobacco plants expressing the coat protein (CP) of an Australian isolate of cucumber mosaic cucumovirus (CMV) were resistant to infection with the homologous and two heterologous CMV strains. The level of resistance observed in CP-expressing plants was related to the virulence of the challenging CMV isolate and not to the similarity between the CP expressed by the transgenic plant and the CP of the challenging virus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cox, D. R. (1972) — Regression models and life-tables (with discussion). Journal of the Royal Statistical Sociey, Series B 34: 187–200.
Edwards, M. C. and Gonsalves, D. (1983) — Grouping of seven biologically defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73: 1117–1120.
Hellwald, K.-H. and Palukaitis, P. (1994) — Nucleotide sequence and infectivity of cucumber mosaic cucumovirus (strain K) RNA2 involved in breakage of replicase-mediated resistance in tobacco. Journal of General Virology 75: 2121–2125.
Horsch, R. B., Fry, J. E., Hoffmann, N. L., Eichholtz, D., Rogers, S. G. and Fraley, R. T. (1985) — A simple and general method for transferring genes into plants. Science 227: 1229–1231.
Jefferson, R. A., Kavanagh, T. A. and Bevan, M. W. (1987) — GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal 6: 3901 -3907.
Kaniewski, W., Lawson, C., Sammons, B., Haley, L., Hart, J., Delannay, X. and Tumer, N. E. (1990) — Field resistance of transgenic Russet Burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8: 750–754.
Kozak, M. (1989) — Circumstances and mechanisms of inhibition of translation by secondary structure in eukaryotic mRNAs. Molecular and Cellular Biology 9: 5134–5142.
Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982) — Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. Cold Sping Harbor, New York.
Nakajima, M., Hayakawa, T., Nakamura, I. and Suzuki, M. (1993) — Protection against cucumber mosaic virus (CMV) strains O and Y and chrysanthemum mild mottle virus in transgenic tobacco plants expressing CMV-O coat protein. Journal of General Virology 74: 319–322.
Namba, S., Ling, K., Gonsalves, C., Gonsalves, D. and Slightom, J. L. (1991) — Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strains. Gene 107: 181–188.
Namba, S., Ling, K., Gonsalves, C., Slightom, J. L. and Gonsalves, D. (1992) — Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses. Phytopathology 82: 940–946.
Nejidat, A. and Beachy, R. N. (1990) — Transgenic tobacco plants expressing a coat protein gene of tobacco mosaic virus are resistant to some other tobamoviruses. Molecular Plant-Microbe Interactions 3: 247–251.
Nelson, R. S., McCormick, S. M., Delannay, X., Dubé, D., Layton, J., Anderson, E. J., Kaniewska, M., Proksch, R. K., Horsch, R. B., Rogers, S. G., Fraley, R. T. and Beachy, R. N. (1988) — Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.
Nitta, N., Masuta, C., Kuwata, S. and Takanami, Y. (1988) — Comparative studies on the nucleotide sequence of cucumber mosaic virus RNA 3 between Y strain and Q strain. Annals of the Phytopathological Society of Japan 54: 516–522.
Owen, J. and Palukaitis, P. (1988) — Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Krology 166: 495–502.
Quemada, H. D., Gonsalves, D. and Slightom, J. L. (1991) — Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: Protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81: 794–802.
Rizos, H. (1995) — Genetic engineering of tobacco plants for resistance to cucumber mosaic virus. PhD Thesis. Macquarie University, Australia.
Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, M. R. (1992) — Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Urology 73: 2099–2103.
Roosinck, M. J. (1991) — Temperature-sensitive replication of cucumber mosaic virus in muskmelon (Cucumis melo cv Iroquis), maps to RNA 1 of a slow strain. Journal of General Virology 72: 1747–1750.
Roossinck, M. J. and Palukaitis, P. (1990) — Rapid induction and severity of symptoms in Zucchini squash (Cucurbita pepo) map to RNA 1 of cucumber mosaic virus. Molecular Plant-Microbe Interactions 3: 188–192.
Stark, D.M. and Beachy, R N. (1989) — Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262.
Van Dun, C. M. P. and Bol, J. F. (1988) — Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167: 649–652.
Weide, R., Koornneef, M. and Zabel, P. (1989) — A simple, nondestructive spraying assay for the detection of an active kanamycin resistance gene in transgenic tomato plants. Theoretical and Applied Genetics 78: 169–172.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rizos, H., Gillings, M.R., Pares, R.D. et al. Protection of tobacco plants transgenic for cucumber mosaic cucumovirus (CMV) coat protein is related to the virulence of the challenging CMV isolate. Australasian Plant Pathology 25, 179–185 (1996). https://doi.org/10.1071/AP96031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1071/AP96031